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Objective Though immunohistochemical study on the effects of Pingxian Powder on N-methyl-D-aspartate receptor1 (NMDAR1) and ?-aminobutyric acid-A receptor ?1 (GABA-AR ? 1 ) in central nervous system of pentylenetetrazol-induced epileptic rats, to explore its possible antiepileptic mechanism. Methods Sixty wistar rats were divided into control group (A), model group (B), phenobarbital group (C) and Pingxian Powder small (D), middle (E), Large (F) dosage group. Pentylenetetrazole (PTZ) was injected into abdomen of rat to reconstruct epileptic kindling model. The total number, percentage of positive area and integral optical density of NMDAR1 and GABA-AR ? 1 of immunoreactive cells in hippocampus CA 1 , CA 3 were studied by the immunohistochemical method and image analyzing system. Results The total number, percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in hippocampus formation of Group B were more than Group A (P
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Objective To investigate the influence of Zengshiling oral liquid on gastric emptying,plasma motilin (MOT) and small intestine propulsion of young rat. Methods Experiment animal were divided into five groups randomly:control group,large dose of Zengshiling oral liquid group,normal dose of Zengshiling oral liquid group,small dose of Zengshiling oral liquid group and Motilium group. The rats were gavaged by 0.9% of NaCl and corresponding drug. 7 days later,gastric emptying was assayed by phenol red,plasma MOT levels and carbon powder propelling rate of small intestine were also assayed. Results The rate of gastric emptying of Zengshiling oral liquid groups were significantly higher than control group (P
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Objective To explore the possible antiepileptic mechanism of Pingxian Powder. Methods In the experiment,Pentylenetetrazole (PTZ) was injected into abdomen of rat to make epileptic kindling model. The total number,percentage of positive area and integral optical density of N-methyl-D-aspartate receptor1 (NMDAR1) and ?-aminobutyric acid-A receptor (GABA-AR?1) of immunoreactive cells in the temporal cortex were studied by the immunohistochemical method and image analyzing system. Results The total number,percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in the temporal cortex of model group were more than normal group (P
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BACKGROUND: The intelligence retardation in children refers to hypo-intelligence structure of full intelligence quotient(FIQ) composed of performance intelligence quotient(PIQ) and verbal intelligence quotient (VIQ) . It is verified in some researches that some of compound formulas provide better intervention on it.OBJECTIVE: To observe the effect of yizhi koufuye on intelligence structure in children with intelligence retardation.DESIGN: Homochronous controlled study based on children.SETTING: Pediatric department in a provincial college of traditional Chinese medicine and one provincial rehabilitation center.PARTICIPANTS: The cases were selected in Rehabilitative Central Hospital in Gansu Province and Clinic of the Hospital Affiliated to Gansu College of Traditional Chinese Medicine from January to July 2002. They were 74 students in Fudu Schools in Chenguan District, Lanzhou City, aged varied from 6 to 16 years, diagnosed as intelligence retardation according to CCMD-Ⅱ-R proposed Intelligence Retardation Diagnostic Standards, and they were able to accept medical and training treatment and were absent of congenital hereditary chromosome disorders. Of those, 47 cases were males and 27 cases females. They were divided into treatment group of 33 cases,the control of 20 cases and simple educational training group of 21 cases.METHODS: In treatment group of 33 cases, yizhi koufuye was administrated and was taken 15 mL each time for children younger than 10 years old, 20 mL for those between 10 to 14 years old and 25 mL for those over 14years old. In addition, the special educational training was applied. In the control of 20 cases, oral application of piracetam was prescribed, 0.4 g per time, 3 times a day and the special educational training was applied additionally. In simple educational training group of 21 cases, the children only received special education same as those in treatment group and the control. Three months made one session. Chinese-Wechsler Intelligence Scale for Children (C-WISC) and specific devices were used to assess FIQ and PIQ and VIQ in classified IQ in 3 groups before and after treatments, and the control comparisons were carried on among groups.MAIN OUTCOME MEASURES: Improvement of FIQ and improved values of VIQ and PIQ in 3 groups.RESULTS: Totally 74 cases were selected and no one lost. Improvement of FIQ: In treatment group, there were 44.7±9.8 and 51.8 ± 11.5 before and after treatment respectively, t = 2. 701, P ＜ 0.01; In the control, there were 43.8 ± 14.0 and 51.0 ± 13.6 before and after treatment respectively, t = 1. 680, P ＞ 0. 05; In simple educational training group, there were 41.6 ± 7.8 and 45.2 ± 10.6 before and after treatment respectively, t = 1. 561 and P ＞ 0.05. Improved values of VIQ: It was 8.1 ± 5.5 in treatment group, 5.3 ± 3.9 in the control and 4. 8 ± 4. 8 in simple educational training group. P ＜ 0.05 compared with the results in treatment group. Improved values of PIQ: It was 4.0 ± 3.6 in treatment group and P ＜ 0. 05 compared with 1.7± 2. 2 in the control and simple educational training group. Improving extent of VIQ was 8. 1 ± 5. 5 and of PIQ was 4.0 ± 3.6 in treatment group, P ＜ 0. 01.CONCLUSION: Yizhi koufuye improves markedly FIQ of children with intelligence retardation and for the classified IQ, the improving extent of VIQ is superior to that of PIQ.
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Objective:To investigate the effects of Yizhi Oral Solution on protein and RNA synthesis of brain tissue of mice. Methods: The radioactive intensities of 3H leucine in brain protein and 3H uridine in RNA of brain tissue of young mice were observed by the methods of incorporation in vivo and group control observation.Results: The radioaetive intensity of 3H leucine of Yizhi Oral Solution group was higher than that of cycloheximide pathological model group ( P
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Objective: To probe and reveal the brain tomic and the wise sharpening action of Fuyuanbunao Granules and Its toxicity and side effect. Methods: The effects of Fuyuanbunao Granules on the memory acquisition disturbance induced by pentobarbital sodium or anisodine, the memory consolidation disturbance induced by sodium nitrite, and the memory repetition disturbance induced by alcohol were observed. Meanwhile, the acute toxicity test was done. Results: The granules could obriously improve the memory disturbance induced by the factors obove, and the improvement effect varied with the dose. The effects of larger dose groups were more obvious. There were no death of animals or toxicity and sile effect in test. Conclusion:Fuyuanbunao Granules maybe have the cholinergic acction. It can promote the aerobic metabolism, improve the cerebral anoxia and the inhibited state of center nervous system. It has low toxicity and can be used in clinical application.