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OBJECTIVE@#To investigate the expression and clinical significance of signal transducer and activator of transcription 3(STAT3) and its target gene c-myc in laryngeal squamous cell carcinoma (LSCC), and the relationship between the two genes.@*METHOD@#Formalin-fixed and paraffin-embedded tissues, which came from 56 cases of LSCC and 30 samples of normal mucosas from 30 patients with total or subtotal laryngectomy over 2.0 cm away from tumor margin, were detected for the expression of STATS, c-myc by in situ hybridization and SP immunohistochemistry. Micro-image analysis system was used to determine the optical density, and the result was analyzed statistically.@*RESULT@#There is overexpression of STAT3, c-myc mRNA and protein in LSCC. The expression of STAT3 and c-myc mRNA in LSCC was associated with clinical stage, differentiation grade and lymph nodal metastases (P < 0.05 or 0.01). The expression of STAT3 and c-myc protein in LSCC was associated with clinical stage and lymph nodal metastases (P < 0.05 or 0.01). There was a positive correlation between the expression of STAT3 and c-myc genes. mRNA and protein, The correlation coefficient (r) was 0.6224 (P < 0.01) for the mRNA expression and 0.7012 (P < 0.01 )for the protein expression.@*CONCLUSION@#The expression of STAT3 and c-myc may play an important role in the tumorigenesis, metastases and poor prognosis of LSCC. There was a positive correlation between the overexpression of STAT3 and c-myc genes in LSCC.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Laryngeal Neoplasms , Metabolism , Pathology , Lymphatic Metastasis , Neoplasm Staging , Proto-Oncogene Proteins c-myc , Metabolism , STAT3 Transcription Factor , MetabolismABSTRACT
Objective To investigate the diagnosis and the effect of microsurgery in patients with acute spontaneous spinal epidural hematoma (ASSEH). Method Five patients with ASSEH treated with microsurgery and confirmed pathologically were retrospectively analyzed. Results The main clinical presentations were root pain and palsy. The main manifestations of MRI were long-segment epidural lesion of high intensity in T1 and T2-weighted images without enhancement. With the microsurgery system, laminectomy via posterior approach and hematoma removal were successfully undergone with full recovery in all cases. Conclusions MRI assisted with the main clinical symptoms may aid preoperative diagnosis in symptomatic ASSEH. Microsurgery is an effective method for treating ASSEH. Postoperative (rather than preoperative) spinal DSA is advantageous for exclusion of spinal vascular malformation in treating ASSEH.
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Objective To investigate the relationship among human papillomavirus(HPV)16/18 and the expression of human telomerase reverese transcriptase(hTERT),c-myc protein in laryngeal squamous cell carcinomas and their significance.Methods The infection of HPV16/18 in 39 laryngeal carcinoma specimens and 10 vocal cord polyp specimens were detected by in situ hybridization.The expression of hTERT protein and c-myc protein in 39 laryngeal carcinoma specimens and 10 vocal cord polyp specimens were detected by immunohistochemistry.Results The positive rate of HPV16/18 infection in 39 laryngeal carcinoma specimens was 48.7%(19/39).The positive rate of HPV16/18 expression in 10 vocal cord polyps was 0(0/10).Statistical tests showed that HPV 16/18 infection was significantly higher in LSCC than that in vocal cord polyps.The positive rate of hTERT protein and c-myc protein expression in 39 laryngeal carcinoma specimens was 84.6%(33/39)and 82.1%(32/39)respectively.The positive rate of hTERT protein and c-myc protein expression in 10 vocal cord polyps was 0(0/10)and 10%(1/10)respectively.Statistical tests showed that hTERT and c-myc protein expression was significantly higher in LSCC than that in vocal cord polyps.Spearman rank correlation analysis revealed that there was significant relation among HPV16/18,hTERT and c-myc protein respectively.Conclusions The results suggest that the expression of hTERT and c-myc protein was associated significantly with the infection of HPV16/18 and they intact each other,which can influent the pathogenesis of laryngeal squamous cell carcinomas.
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Objective To study the correlation between expression of vascular endothelial growth factor(VEGF)and tissue inhibitor of metalloproteinase 2(TIMP-2)and prognosis of patients with laryngeal squamous cell carcinoma.Methods The expression of VEGF and TIMP-2 were measured in 38 specimen of LSCC and 19 specimens of normal laryngeal tissue adjacent to the tumors with EnVision immunohistochemical technique,follow-up for 3~5 years after operation local relapse occurred in four cases,cervical nodular metastasized occurred in fwo cases and pneumonic metastasis occurred one case.Results The expression level of two marks in primary tumors was significantly higher than that in pericancerous tissues(P
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Objective To investigate the correlation between human papillomavirus(HPV) 16/18 subgroup infection and laryngeal squamous cell carcinoma(LSCC).Methods The infection of HPV16/18 in 39 laryngeal carcinoma specimens and 10 vocal cord polyp specimens were detected by in situ hybridization. Results The positive rate of HPV16/18 expression in 39 laryngeal carcinoma specimens was 48.7%(19/39). The positive rate of HPV16/18 subgroup expression in 10 vocal cord polyps was 0(0/10) . Statistical test showed that HPV16/18 subgroup infection was significantly higher in LSCC than that in vocal cord polyps. No statistically association was observed among the frequency of HPV16/18 subgroup infection and TNM stages, degree of differentiation or lymph nodes metastases. Conclusions HPV 16/18 subgroup infection is associated with he pathogenesis of LSCC.
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OBJECTIVE To investigate the application of near-total laryngectomy on the treatment of advanced laryngeal and hypopharyngeal carcinoma. METHODS Thirty patients with laryngeal carcinoma(T3N0～T3N1 17cases,T4N0～T4N1 13cases) and nine with hypopharyngeal carcinoma(T3N0～T3N1 6cases,T4N0～T4N1 3cases) were treated with near-total laryngectomy from May 1995 to March 2003 according to Peaeson modifying method of Tangpingzhang. Concurrent neck dissection were performed in sixteen patients. RESULTS The speech function was obtained in 36 cases (92.3 %) and good swallowing function was obtained in 38 cases(97.4%). Three year survival rates were 73.3% (22/30) in laryngeal carcinoma and 55.6 %(5/9)in hypopharyngeal carcinoma and five year survival rate in laryngeal carcinoma was 61.1%(11/18) . Local recurrence rate was only 2.56 %. CONCLUSION Near-total laryngectomy and speech rehabilitation can be used in most cases that might be treated with total laryngectomy in the past. It can provide satisfying survival rate, good swallowing and speech function.
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Objective To investigate the incidence,original location and clinical features of olfactory sulcus polyp in patients with chronic sinusitis.Methods Two hundred and seventeen cases(351 sides) with chronic sinusitis were reviewed,their olfactory sulcus was carefully examined with nasal endoscope during operation.The incidence and original location of olfactory sulcus polyp were observed,and the relationship between olfactory sulcus polyps and prognosis of chronic sinusitis or olfaction of the patients with olfactory sulcus polyps were also statistically analyzed with Chi-square test and Spearman rank correlation.Results The incidence of olfactory sulcus polyp in the cases with chronic sinusitis was 18.52%.Among these cases with olfactory sulcus polyps,the rate of olfactory disorder was 81.82%,which was higher than that in the cases without olfactory sulcus polyps(25.63%,P