ABSTRACT
<p><b>BACKGROUND</b>To improve the efficacy and selectivity of gene therapy for lung cancer through inducing oncostatin M (OSM) gene expression by radiation via the early growth response gene-1 (Egr-1) promoter.</p><p><b>METHODS</b>The radio-inducible OSM gene was constructed by insertion of Egr-1 promoter into upstream of the OSM gene. The expression of OSM in lung adenocarcinoma cell line A549 which was transfected with pEO and exposed to different doses of γ-ray irradiation was analyzed, and the relative survival fraction of cells and cell survival curve were observed. To examine the efficacy of this pEO gene therapy in vivo, the tumor supression effects were investigated in 40 nude mice bearing lung tumors.</p><p><b>RESULTS</b>Expression of OSM gene in A549 cells transfected with pEO plasmids was markedly upregulated in a radiation dose-dependent manner. A gene therapy experiment in vitro showed that pEO transfected A549 cells became highly sensitive to ionizing radiation. pEO transfected tumors regressed significantly after a combination therapy with irradiation in all mice (n=10), and three tumors disappeared in 3 weeks without any side effect.</p><p><b>CONCLUSIONS</b>The results indicate that tumor targeted expression of OSM gene under the control of a radio-inducible promoter represents a novel strategy for safe and effective gene therapy for lung cancer and might be widely applied in the future.</p>
ABSTRACT
Objective To investigate the anti-angiogenesis and tumor inhibitory effects of recombinant human endostatin(rhES)combined with paclitaxel-cisplatin chemotherapy(TP regimen)on xenograft tumors in nude mice of human breast carcinoma cell MCF-7.Methods Forty-eight xenograft nude mice were randomized into group A and group B,then every group were divided 4 subgroups,that is,TP group(cisplatin 20 mg/kg at days 1,7 and 14,and paclitaxel 5 mg/kg at days 1 and 7,i.p.),rhES group(rhES 10 mg?kg-1?d-1 for 14 d,i.v.),rhES+TP group(combined treatment as above-mentioned),and control group(normal saline,i.v.).Inhibitory rate of xenograft and tumor growth curve was calculated and plotted.Serum VEGF level was determined by ELISA,microvessel density(MVD)in tumors was measured by immunohistochemistry,and cell apoptosis was examined by TUNEL staining.Survival time was observed in group B.Results Serum VEGF and MVD was significantly lower in rhES+TP group than in other groups(P
ABSTRACT
Objective To improve quota-sampling precision and reproducibility of the impedance blood rheometer. Methods Quota-sampling control circuit and the sampling needle's structure were improved for the early MVIS blood rheometer. The parameters of sampling were analyzed. Results After the improvement, the sampling errors were reduced obviously (less than ?3%). The quota-sampling handling was more accurater. Conclusion The precision and reproducibility of the blood rheometer are ensured.