ABSTRACT
Objective:To extract DNA from Bifidobacteria and E.coli cells and incubate DNA with IEL to observe.DNA's activation for IEL.Also plan to use the DNA to immune mice to observe the change of immunologic function,discuss the effect of Bifidobacteria and E.coli's whole DNA to murine and make a comparision study.Methods: Mice of Bifidobacteria DNA and E.coli's DNA treated groups are respectively given with DNA by muscular and subcutaneous injection. Detect the immunologic function of spleen cell, extract IEL cells and incubate them with DNA, detecting IEL activity and the production of cytokine. Results:The activities of IL 2 and NK are all significantly higer than those of in control groups (P
ABSTRACT
In this paper,we reported that T cell,T cell subpopulation and natural killer(NK)cell activities of peripheral blood lymophocytes from the Fatients with tumor weredetected with OKT monoclonal antibody and ~(51)Cr release cytotoxity test.The resultsshowed that percentage of OKT3~+,OKT4~+ cell in the peripheral blood of patients withtumor are low,OKT8~+ is high and the ratio of OKT4/OKT8 is low.The results haveproved that the ratio of OKT4/OKT8 and NK cell activities were significantly low inthe peripheral blood of patients with tumor.However,the activities have restored tonormal or near normal level in the patients after surgical operation for more that twoyears with no recurrence. Our data suggest that detecting of the ratio of OKT4/OKT8 and activities of NKcell might be valuable to the prognosis of patients with tumor.
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Objective:To study comparitively the iIEL activation of somatic polysaccharose of bifidobacteria and E.coli.Methods:Intestinal intraepithelial lymphocyte(iIEL)is isolated from small intestine using Percoll discontinuous density gradient centrifugation At the same time, somatic polysaccharose of bifidobacteria and E.coli are isolated by organic solvent and are cultured with iIEL,then the natural killing activity of iIEL and ability to produce interleukin 2(IL 2)were examined Results:Somatic polysaccharose of bifidobacteria and E.coli significantly enhance natural killing activity of iIEL and its ability to produce IFN? and IL 2 (P