ABSTRACT
Objective:To investigate the expression and clinical significance of endostatin (ES) and angiostatin (AS) in pterygium.Methods:From January 2016 to December 2018, 60 cases (60 eyes) of pterygium tissue and 60 cases (60 eyes) of normal human conjunctival tissue were selected from the eye surgery of Traditional Chinese Medicine Hospital of Yiwu.HE staining was used to observe the morphological changes of pterygium and normal conjunctival tissue.Western blot was used to measure ES and AS protein levels in the tissues of pterygium group and control group.Results:HE staining showed that in the normal bulbar conjunctival tissue, the stromal layer was connective tissue and the epithelial layer was columnar epithelium; in the pterygium, the basal layer had a large number of new blood vessels, fibroblasts, collagen fibers, and inflammatory cells infiltrated around the blood vessels; the epithelium showed different degrees of hyperplasia.The protein levels of ES and AS in pterygium tissues[(0.35±0.12), (0.62±0.17)] were higher than those in the control group [(0.13±0.08), (0.16±0.09)]( t=11.816, 18.524, P=0.000, 0.000). The protein levels of ES and AS in the pterygium tissues of the recurrent group [(0.63±0.15), (0.87±0.21)] were higher than those in the initial group [(0.22±0.11), (0.45±0.16)]( t=17.073, 12.323, P=0.000, 0.000). There was positive correlation between ES and AS in pterygium ( r=0.571, P=0.000). Conclusion:The levels of ES and AS in pterygium tissue are increased, and ES and AS may be involved in the occurrence and recurrence of pterygium.
ABSTRACT
Objective To investigate the correlation between human papilloma virus(HPV) 16/18 infection and the expression of Rb and p16 protein in bladder cancer tissue,and to analyze the relationship between HPV infection and the incidence of bladder cancer.Methods The expression of HPV16/18 E6 and E7 gene encoded protein,RB and p16 were detected by immunohistochemical method in 40 cases of bladder cancer and 40 cases of normal bladder tissues,and the correlation between them and pathological grading,stage of international union of cancer(UICC),whether recurrence or not after receiving surgery was analyzed.Results In bladder cancer tissues,HPV16/18 E6 and E7 gene encoded protein,RB and p16 positive rates were 65%,47.5%,42.5%,compared with the positive rate of normal bladder tissue samples(22.5%,92.5%,87.5%),the differences were statistically significant(P0.05).The expression of HPV16/18 E6 and E7 gene encoded protein and Rb,p16 protein were not significantly correlated(P>0.05),The expression of Rb and p16 protein were negatively correlated(P<0.05).Conclusion HPV 16/18 infection is related to the occurrence and development of bladder cancer,but its mechanism might not be related to the abnormal expression of Rb and p16 protein.
ABSTRACT
Sphingosine-1-phosphate(S1P) is an important bioactive lipid produced from cell membrane sphingomyelin metabolism process.S1P and cell membrane surface S1P receptors(S1PR1-5) are G protein coupled receptors(GPCRs), which influence the formation of new blood vessels in the immune system via combining the related inflammatory signaling pathway.This review describes briefly the effects of S1P and S1PRs on autoimmune disease angiogenesis through intracellular signal transduction, such as rheumatoid arthritis, multiple sclerosis, colitis, systemic lupus erythematosus.Further research will be a new therapeutic target on vascular inflammation of autoimmune diseases.