ABSTRACT
Objective To investigate the values of combined determination of pleural effusion and serum carcinoembryonic antigen (CEA), cytokeratin 19 fragment (CYFRA21-1), neuron-specific enolase (NSE), and squamous cell carcinoma antigen (SCC-Ag) in diagnosis of lung cancer. Methods 101 patients with pleural effusion were reviewed retrospectively. Expressions of CEA, CYFRA21-1, NSE and SCC-Ag in pleural fluid and serum were detected; the optimum cut-off points resulting from the best sensitivity-specificity balance in the receiver operator characteristic (ROC) curves were constructed. The expressions of the tumor markers were compared among different pathological types of lung cancer. Results The levels of tumor markers in both pleural effusion and serum were significantly higher in patients with lung cancer than the benign group (P < 0.05). The expression levels and positive rate of CEA for lung adenocarcinoma, NSE for small cell lung cancer, and CYFRA21-1and SCC-Ag for pulmonary squamous cell carcinoma were higher than any other single detection (P < 0.05). Combined detection of the tumor markers in pleural fluid and serum improved the detection rate of lung cancer. Conclusions Combined detection of pleural effusion and serum tumor markers has important reference value in the detection of lung cancer and in pathological typing.
ABSTRACT
In order to study the cytocompatibility of nanophase hydroxyapatite ceramic in vitro, we prepared hydroxyapatite by use of the wet chemistry techniques. The grain size of hydroxyapatite of interest to the present study was determined by scanning electron microscopy and atomic force microscopy with image analysis software. Primary culture of osteoblast from rat calvaria was established. Protein content, synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase hydroxyapatite ceramics and on conventional hydroxyapatite ceramics for 7, 14, 21 and 28 days were examined. The results showed that the average surface grain size of the nanophase and that of the conventional HA compact formulations was 55 (nanophase) and 780 (conventional) nm, respectively. More importantly, compared to the synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase was significantly greater than that on conventional ceramics after 21 and 28 days. The cytocompatibility was significantly greater on nanophase HA than on conventional formulations of the same ceramic.
Subject(s)
Animals , Rats , Alkaline Phosphatase , Metabolism , Biocompatible Materials , Chemistry , Cell Adhesion , Cells, Cultured , Ceramics , Chemistry , Durapatite , Chemistry , Microscopy, Atomic Force , Microscopy, Electron, Scanning , Nanostructures , Chemistry , Osteoblasts , Cell BiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of small dose of dopamine on the intestinal mucosa of scalded rats in shock stage.</p><p><b>METHODS</b>Wistar rats inflicted by 30% TBSA of III degree scalding were employed as the model. The rats were pre-placed with cardiac catheter before and were resuscitated intravenously after injury. The scalded rats were treated by routine (control) and small dose of dopamine (3 micro g.kg(-1).min(-1)), respectively. The changes of rat serum levels of diamine oxidase (DAO), malondialdehyde (MDA) and lactic acid (LA) were observed after treatment. And the pathomorphological changes of the intestine were scored.</p><p><b>RESULTS</b>The general condition of the rats with dopamine treatment in shock stage was better than that in control group. The rat serum levels of MDA, LA and DAO decreased obviously, especially during 3 to 12 postburn hours (PBHs) after treatment by small dose of dopamine. The pathomorphological scoring of ileum in dopamine treating group was better than that in control.</p><p><b>CONCLUSION</b>The intestinal mucosa of severely scalded rats in shock stage could be well protected by small dose of dopamine.</p>
Subject(s)
Animals , Male , Rats , Burns , Pathology , Disease Models, Animal , Dopamine , Pharmacology , Intestinal Mucosa , Physiology , Protective Agents , Pharmacology , Rats, WistarABSTRACT
Objective: To study the cytocompatibility of nanophase alumina ceramics with osteoblasts. Methods: Alumina ceramics were prepared via wet chemistry techniques. The grain size of alumina of interest in the present study was determined by scanning electron microscopy and atomic force microscopy with image analysis software. Primary osteoblast culture was established from rat calvaria. Protein content, synthesis of alkaline phosphatase(ALP) and deposition of calcium-containing mineral by osteoblasts cultured on nanophase alumina ceramics and conventional alumina ceramics for 7, 14, 21 and 28 d were respectively examined. Results: The average surface grain size of the nanophase and conventional alumina compact formulations was 60 nm and 1.80 ?m respectively.Synthesis of ALP and deposition of calcium-containing mineral were significantly greater by osteoblasts cultured on nanophase than those on conventional ceramics after 21- and 28- day culture. Conclusions: Nanophase alumina may stimulate ALP synthesis and calcium deposition of osteoblasts.