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Aim To investigate the effects of myricetin on the migration and invasion of hepatoma SMMC-7721 cells.Methods SMMC-7721 cells were treated with different concentrations of myricetin.Migration and invasion of SMMC-7721 cells were examined by Wound healing and Transwell, and the expression of E-cadherin and N-cadherin was detected by RT-qPCR and Western blot.Results Myricetin may inhibit the viability of SMMC-7721 cells via different concentration(10~40 μmol·L-1);furthermore, myricetin might also inhibit the migration and invasion of hepatoma SMMC-7721 cells.Meanwhile with the increasing of myricetin concentration, both filopodia and lamellipodia formation was reducd, and 7721 cells displayed in more integrity.And data from RT-qPCR and Western blot demonstrated that myricetin may up-regulate the expression of E-cadherin, simultaneously, down-regulate N-cadherin in SMMC-7721 cells.Conclusion Myricetin may influence the cell migration and invasion through up-regulating the expression of E-cadherin, simultaneously, down-regulating N-cadherin and activate cytoskeleton remolding of SMMC-7721.
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OBJECTIVE: To investigate the effect of Compound Rhubarb Mixture on contraction of isolated small intestinal smooth muscle of rabbit. METHODS: Serum containing Compound Rhubarb Mixture was prepared. The contraction curves of isolated small intestinal smooth muscle before and after administration of the serum were recorded by Medlab biological information collecting system. RESULTS: The serum containing Compound Rhubarb Mixture amplified the contraction amplitude of isolated small intestinal smooth muscle of rabbit. The rate of change of contraction amplitude was elevated significantly after administration, while the frequency of contraction did not change obviously. CONCLUSION: The serum containing Compound Rhubarb Mixture can enhance the contractive function of isolated small intestinal smooth muscle of rabbit.
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AIM To examine the effect and mechanism of emodin on intestinal movement of constipation model. METHODS The movement of charcoal powder and the absorption of D-xylose into serum were observed. The electrical p otential difference(PD) related to Na + and glucose transport was measured across the wall of rev erted intestinal sacs. RESULTS Emodin 0 1, 0 2 g?kg -1 incr eased markedly the rate of charcoal powder moving and inhibited the absorption of D-xylose into serum. Emodin 0 2, 0 4, 0 8 and 1 6 g?L -1 decreased PD when ther e was glucose. However, emodin had little effect when glucose was free. CONCLUSION Emodin promotes intestinal movement and this effect is related to Na +,K +-ATPase.