ABSTRACT
The roots, barks, branches and pericarps of Juglans mandshurica were used as folk medicine in China and reputed for its treatment of several cancers, such as gastric cancer, liver cancer and leukemia. The extracts of the roots, branches, leaves and pericarps of J. mandshurica have been experimentally proved to show anti-tumor activities. Tannins, which exhibited antioxidant and anti-tumor activities, were the main constituents in J. mandshurica. In this paper, a simple spectrophotometric method was developed for the determination of total tannins in the roots, branches, leaves and pericarps of J. mandshurica collected in Dalian and Anshan of Liaoning Province. Gallic acid was used as standard compound and the content of total tannins was calculated as gallic acid equivalent. As a result of the method validation, a good linearity (r = 0.9997, n = 5) and a high recovery of gallic acid (99.02%, RSD 3.7%, n = 9) was achieved. Eight samples including four parts of J. mandshurica collected in two places were analyzed for their total tannins with the established method. In the corresponding parts of J. mandshurica, except the pericarps, the contents of total tannins showed no significant difference between samples collected in Dalian and Anshan, while the content of total tannins in different parts of J. mandshurica were significantly different. The average content of total tannins in the roots, branches, leaves and pericarps of samples collected in Dalian and Anshan was 45.66, 23.40, 58.24, 3.58 mg g(-1), respectively.
Subject(s)
Juglans , Chemistry , Plant Extracts , Plant Leaves , Chemistry , Plant Roots , Chemistry , Plant Stems , Chemistry , TanninsABSTRACT
<p><b>OBJECTIVE</b>To establish the HPLC fingerprint method of Fructus Polygoni Orientalis before and after processed by choosing taxifolin as reference to compare the changes of chemical composition.</p><p><b>METHOD</b>HPLC method was emplyed with a gradient elution phase in a flow rate of 1 mL x min(-1) and the detection wavelength of 270 nm.</p><p><b>RESULT</b>Twenty marker peaks were marked out in the raw samples and 33 marker peaks in the processed product. Methodology met was consistent with the requirement, similarity was exceeded 0.9.</p><p><b>CONCLUSION</b>This method is stationary, precise and feasible, which provide references of quality control for Fructus Polygoni Orientalis.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drug Contamination , Drugs, Chinese Herbal , Chemistry , Plant Extracts , Chemistry , Quality Control , Quercetin , Reference Standards , Spectrometry, Mass, Electrospray Ionization , MethodsABSTRACT
OBJECTIVE:To provide references for the quality assessment of traditional Chinese liquid medicine-Fructus Polygoni Orientalis.METHODS:The description and the physicochemical identification of Fructus Polygoni Orientalis were carried out;the contents of the extractives were determined and the chemical composition of which was studied.The quality of commercial Fructus Polygoni Orientalis medicinal substances from15nationwide areas was investigated.RESULTS:The results showed that the content of the preparation was higher than that of the raw material in the water soluble extractive assaying;the content of the alcohol-soluble extractive was the opposite.Meletin was found to be contained in the Fructus Polygoni Orien?talis acetic ether extraction liquid by the TLC identification.There was only half maturing rate for most of the commercial Fructus Polygoni Orientalis.CONCLUSION:This method can be used as a reference for the quality assessment of Fructus Polygoni Orientalis.
ABSTRACT
AIM:This study was concerned with the effect of Alexipharmie Capsule(Flos Lonicerae Japonicae,Fructus Forsythiae,Radix Scutellariae,Radix Isatidis,Herba Ephedrae,Radix et Rhizoma Glycyrrhizae) on fever,inflammation,pain and cough. METHODS: We adopted ear swelling mouse model induced by xylene and foot swelling mouse model induced by carrageenan to observe the anti-inflammatory effects;took 15% beer yeast and bacterial endotoxin as pyrogen to study the antipyretic effects on rats and rabbits;used acetic acid stretching method to observe the analgesic effects;adopted strong aqua ammoniae spraying and sulfur dioxide stimulus method to study the antitussive effects. RESULTS: Alexipharmic Capsule could obviously inhibit ear and foot swelling of mouse model(P