Glutamine synthetase-negative hepatocellular carcinoma has better prognosis and response to sorafenib treatment after hepatectomy / 中华医学杂志(英文版)

BACKGROUND@#Glutamine synthetase (GS) and arginase 1 (Arg1) are widely used pathological markers that discriminate hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma; however, their clinical significance in HCC remains unclear.@*METHODS@#We retrospectively analyzed 431 HCC patients: 251 received hepatectomy alone, and the other 180 received sorafenib as adjuvant treatment after hepatectomy. Expression of GS and Arg1 in tumor specimens was evaluated using immunostaining. mRNA sequencing and immunostaining to detect progenitor markers (cytokeratin 19 [CK19] and epithelial cell adhesion molecule [EpCAM]) and mutant TP53 were also conducted.@*RESULTS@#Up to 72.4% (312/431) of HCC tumors were GS positive (GS+). Of the patients receiving hepatectomy alone, GS negative (GS-) patients had significantly better overall survival (OS) and recurrence-free survival (RFS) than GS+ patients; negative expression of Arg1, which is exclusively expressed in GS- hepatocytes in the healthy liver, had a negative effect on prognosis. Of the patients with a high risk of recurrence who received additional sorafenib treatment, GS- patients tended to have better RFS than GS+ patients, regardless of the expression status of Arg1. GS+ HCC tumors exhibit many features of the established proliferation molecular stratification subtype, including poor differentiation, high alpha-fetoprotein levels, increased progenitor tumor cells, TP53 mutation, and upregulation of multiple tumor-related signaling pathways.@*CONCLUSIONS@#GS- HCC patients have a better prognosis and are more likely to benefit from sorafenib treatment after hepatectomy. Immunostaining of GS may provide a simple and applicable approach for HCC molecular stratification to predict prognosis and guide targeted therapy.

Relationship between nutritional status and growth and development of children aged 1-12 / 公共卫生与预防医学

Objective To analyze the nutritional status and growth and development of children aged 1 to 12 years old, and to provide guidance for subsequent child health care work. Methods A total of 514 children aged 1 to 12 years old underwent physical examination in our hospital were enrolled, data including weight, height, bone mineral density, serum vitamin A, vitamin D and blood trace elements were detected, thereafter, Pearson correlation analysis was used to discuss the correlation between children's growth and development and serum trace elements. Results Among the 514 children in this study, there were 224 boys and 290 girls, including 86 children in early childhood group, 165 children in preschool age group and 263 children in school age group. The prevalence of vitamin A deficiency was 7.98% (41/514), with a significant difference among the different age groups, including 22.09% (19/86) in early childhood group, 7.88% (13/165) in preschool age group, and 4.56% (12/263) in school age group. The height and weight of children with vitamin A deficiency were lower than those of children in the same age group, and there was a positive correlation between serum vitamin A concentration and height and weight of children (P<0.05). The detection rate of vitamin D deficiency was 12.06% (62/514), with 16.07% (36/224) in girls, higher than 8.97% (26/290) in boys. The height of children with vitamin D deficiency was significantly lower than that of children with normal vitamin D levels (P<0.05), and the incidence of bone mineral deficiency was significantly higher than that of that with normal vitamin D levels. Serum vitamin D concentration was positively correlated with height in children (P<0.05) and negatively correlated with bone mineral deficiency (P<0.05). In addition, there were 51 children (9.92%) with hypocalcemia. Serum calcium levels were significantly lower in boys than in girls (P<0.05), and the incidence of hypocalcemia was significantly higher in boys than in girls (P<0.05).Serum calcium level was positively correlated with height (P<0.05), and negatively correlated with bone mineral deficiency (P<0.05). There were 35 cases (6.81%) of children with hypoglycemic zinc and 32 cases (6.23%) of children with hypoglycemic iron, and there was a positive correlation between serum zinc and iron levels and children's height and weight (P<0.05). Conclusions Vitamin A deficiency is more serious in younger children than in older children, and vitamin A deficiency affects children's height and weight; vitamin D deficiency is more serious in girls than in boys, and vitamin D deficiency affects children's bone density. Vitamin D supplementation and outdoor activities for girls are recommended. In addition, deficiencies in trace vitamins, including blood calcium, zinc and iron, will also affect children's height and weight, and a proper diet is recommended to supplement the body with essential elements.

Malignant tumor and hyperfibrinogenemia / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the blood fibrinogen (FIB) content before and after chemotherapy or radiotherapy in 43 malignant tumor patients.</p><p><b>METHODS</b>COULTER ACL-200 automated coagulation analyzer was used in monitoring FIB in the patients.</p><p><b>RESULTS</b>The FIB content was significantly higher in malignant tumor patients than that in benign disease patients. Significant reduction was observed after treatment which became elevated again when there was recurrence or metastasis.</p><p><b>CONCLUSION</b>Assessment of FIB not only helps to diagnose cancers but evaluate the therapeutic effect and prognosis also.</p>