ABSTRACT
Objective To investigate the effect of electroacupuncture at point Zusanli (ST36) on gastric expression of heat shock protein 70 (HSP70) mRNA in rats with stress gastric ulcer and explore the molecular mechanism of its prevention and treatment of gastric ulcer. Method Fifty-one SD rats were randomly allocated to normal control (normal), stress gastric ulcer (model) and electroacupuncture at point Zusanli (electroacupuncture) groups. A model of stress gastric ulcer was made by cold restraint. From the second day after model making, the electroacupuncture group received electroacupuncture at point Zusanli 20 min once daily for three consecutive days, with left and right points Zusanli alternately needled; the model group, the same restraint treatment as in the electroacupuncture group; the normal group, no treatment. Gastric mucosal ulcer index (UI) was calculated using the GUTH’s method. The pathological morphology of gastric tissue was observed by HE staining. Gastric HSP70 mRNA expression was determined by RT-PCR. Result Different numbers and sizes of obvious gastric mucosal ulcers were observed, UI increased significantly (P<0.001) and HSP70 mRNA expression decreased significantly (P<0.05) in the model group compared with the normal group. The pathological change in gastric tissue improved somewhat, gastric mucosal UI decreased significantly (P<0.05) and HSP70 mRNA expression increased significantly (P<0.01) in the electroacupuncture group compared with the model group. Conclusion Electroacupuncture at point Zusanli can upregulate gastric HSP70 mRNA expression and promote the repair of gastric mucosa injuries in rats with stress gastric ulcer.
ABSTRACT
Objective To observe the effect of electroacupuncture(EA) of different acupoints on electroretinogram(ERG) and cerebral visual evoked potentials(VEP) in healthy subjects so as to evaluate the relative specificity of the function of acupoints.Methods A total of 33 healthy adult volunteers were observed in the present study.The subject was asked to lie on a testing bed for a while,two disk electrodes were respectively fixed to the suborbital region and the occiput(the crossing point,5cm apart from the right median line of the head and 5cm above the ear),and the reference electrode was fixed to the medial side of the earlobe for recording ERG and cerebral VEP separately.The testing room was asked to keep faint light during the whole recording process.A flash stimulation(frequency 1 Hz) was applied to the subject's eye(with a distance of about 50 cm between the light source and the tested eye,and the other eye was covered by an eyepatch).EA(0.5 Hz,0.2 ms and 3-4 V) was applied to Guangming(GB 37),Neiguan(PC 6) and Zusanli(ST 36) for 30 min respectively in different testing days(with the interval being 3 days at least).ERG and cerebral VEP were recorded by using Polygraphy(RM-6200).Results Following EA of Guangming(GB 37),Neiguan(PC 6) and Zusanli(ST 36),the changing values(increase and decrease) of the amplitude of b waves of ERG and P100 waves of cerebral VEP were(19.68?12.61)% and(12.84?14.08)%,(6.09?14.06)% and(5.61?8.01)%,(2.39?3.52)% and(5.43?8.22)%,respectively,while those of b waves of ERG and P100 waves of cerebral VEP of GB 37 were significantly bigger than those of PC 6 and ST 36(P