ABSTRACT
Objective To explore the expression of microRNA (miR)-143 and related association to clinicopathologic features of cervical cancer in both Uygur and Han women with cervical cancer in Xinjiang.Methods The expression levels of miR-143 in the specimens from 34 non-tumor and 66 cervical cancer tissues,were examined by quantitative real-time PCR.Levels on the expression of miR-143 in Xinjiang Uygur women and the correlations between the expression levels of miR-143 and related clinicopathologic features of cervical cancer were analyzed.Results The expression levels of miR-143 were significantly lower in the tumor tissues than that in the non-tumor tissues (P＜0.05) but no significant difference was found between women with Uygur or Han ethnicities.Down-regulated miR-143 expression was associated with both the tumor size and lymph node metastasis in patients with cervical cancer (Z=-2.628,P=0.009 and Z=-2.127,P=0.033 respectively).No significant associations were found between the expression levels of miR-143 and factors as age,depth of tumor invasion,parametrial infiltration,clinical stage,types of histology and stage of differentiation.ROC curve analysis on miR-143 expression in cervical cancer patients with different tumor sizes and lymph node metastasis:the rates on AUC were 0.711 and 0.697,both larger than 0.5,respectively.The sensitivity and specificity of evaluating tumor size were 85.7％ and 62.2％.The sensitivity and specificity of lymph node metastasis were 72.2％ and 60.4％.Conclusion It seemed that miR-143 play an important role in the processes of generation and progression of cervical cancer.However,there was no significant difference found between the different ethnic groups.The expression level of miR-143 might serve as a valuable adjuvant parameter for diagnosing and predicting the state of cervical cancer.
ABSTRACT
<p><b>BACKGROUND</b>Atherosclerosis is an important cardiovascular disease, becoming a major and increasing health problem in developed countries. However, the possible underlying mechanisms were not completely clear. In 2009, our research group first discovered that hydrogen sulfide (H(2)S) as a novel gastrotransmitter played an important anti-atherosclerotic role. The study was designed to examine the regulatory effect of hydrogen sulfide (H(2)S) on endoplasmic reticulum stress (ERS) in apolipoprotein E knockout (apoE(-/-)) mice fed a Western type diet.</p><p><b>METHODS</b>C57BL/6 mice and homozygous apoE(-/-) mice were fed a Western type diet. C57BL/6 mice were injected intraperitoneally with normal saline (5 ml/kg per day) as control group. The apoE(-/-) mice were treated with the same dose of normal saline as the apoE(-/-) group, injected intraperitoneally with sodium hydrosulfide (NaHS, an H(2)S donor, 56 µmol/kg per day) as the apoE(-/-) + NaHS group and injected intraperitoneally with DL-propargylglycine (PPG, a cystathionine-γ-lyase inhibitor, 50 mg/kg, per day) as the apoE(-/-) + PPG group. After 10 weeks, the mice were sacrificed and the plasma lipids were detected. Sections of aortic root from these animals were examined for atherosclerotic lesions by HE and oil red O staining. The aortic ultrastructure and microstructure were analyzed with the help of light and electronic microscope. Glucose-regulated protein 78 (GRP78), caspase-12, copper-andzinc-containing superoxide dismutase (Cu/ZnSOD) and Mn-containing superoxide dismutase (MnSOD) protein expression in aortic tissues were detected with immunohistochemistry. The level of intracellular reactive oxygen species (ROS) were measured by using a commercial assay kit.</p><p><b>RESULTS</b>Compared with control mice, apoE(-/-) mice showed increased plasma levels of total cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL), decreased high density lipoprotein (HDL), increased aortic plaque size, destroyed ultra-structure of aortic tissue, and increased expression of GRP78 and caspase-12 proteins. Compared with apoE(-/-) mice, H(2)S donor-treated apoE(-/-) mice showed a decreased plasma LDL level, lessened plaque necrosis and attenuated aortic ultra-structural disorder. H(2)S donor-treatment induced GRP78 expression but suppressed caspase-12 expression in aortic lesions. However, compared with apoE(-/-) mice, PPG treated apoE(-/-) mice showed enlarged plaque size, more severe ultrastructural disorder of the aortic tissue and reduced GRP78 staining in aortic lesions. The plasma lipids and the staining of caspase-12 in apoE(-/-) + PPG rats did not significantly differ from those in the apoE-/-mice. Consistently, H(2)S induced SOD expression, accompanied by a reduced level of ROS.</p><p><b>CONCLUSION</b>H(2)S plays a regulatory role in aortic ERS and reduces atherosclerotic lesions in apoE(-/-) mice fed with a Western type diet.</p>