ABSTRACT
Objective To examine the effect of miR-301b in the regulating the differentiation of mesenchymal stem cells into adipocytes.Methods Murine ST2 stromal cells were isolated,cultured and induced with adipogenic agents.The expression of miR-301b was detected by qRT-PCR in adipogenic differentiation group and control group.Stromal ST2 cells were transfected with miR-301b mimics followed by adipogenic treatment.qRT-PCR and Western blotting were conducted to detect the changes of adipocyte specific genes and protein expression levels in the miR-301b mimics transfection group and negative control(NC)transfection group. Results qRT-PCR showed that the expression of miR-301b was decreased after adipogenic treatment in ST2 cells.The relative expression level of miR-301b was less in the adipogenic differentiation group (0.219 ± 0.021) than that of the control group (1.000 ± 0.425, P<0.05). The relative expressions of peroxisome proliferator activated receptor γ(PPARγ),CCAAT/enhancer-binding protein α(C/EBPα)and adipocyte fatty acid binding proteins(aP2)were lower in the miR-301b mimics transfecting group than those in the NC transfecting group(P<0.05).The protein levels of the marker gene aP2 and transcription factors PPARγ and C/EBPα decreased in miR-310b mimics transfecting group compared with those of the NC transfecting group (P<0.05). Conclusion miR-301b can reduce adipocyte differentiation.