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Chinese Journal of Biotechnology ; (12): 306-310, 2006.
Article in Chinese | WPRIM | ID: wpr-286291

ABSTRACT

To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.


Subject(s)
Animals , Cattle , Female , Cellular Reprogramming , Cloning, Organism , Methods , Coculture Techniques , Culture Media , Embryo, Mammalian , Cell Biology , Embryonic Development , Epithelial Cells , Cell Biology , Fallopian Tubes , Cell Biology , Granulosa Cells , Cell Biology , Nuclear Transfer Techniques
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