Antibacterial activity of TiO2 nanotube arrays on periodontal pathogens before and after loaded with minocycline hydrochloride / 中国病理生理杂志

AIM:To investigate the effects of titanium dioxide(TiO2)nanotube arrays on the early adhesion behavior of Porphyromonas gingivalis(Pg),Tannerella forsythia(Tf)and Actinobacillus actinomycetemcomitans(Aa)be-fore and after loaded with minocycline hydrochloride(MN).METHODS: TiO2nanotube arrays were prepared by ano-dization and loaded with MN.Titanium slices were divided into 3 groups according to different treatment methods: pure polishing titanium(Ti)group,TiO2nanotube titanium(TiO2)group, and MN(120 μg)TiO2nanotube titanium(MN TiO2)group.The antibacterial properties of the titanium tablets were evaluated by the bacteriostasis test.RESULTS:The Ti had no antibacterial activity.The antibacterial activity of TiO 2to Aa,Pg and Tf was poor,with only about 20%of anti-bacterial rate after 4 h.After loaded with MN,its antibacterial activity was enhanced,and the antibacterial rate was up to 77%after 4 h.CONCLUSION: No antibacterial activity in the Ti group was observed.If TiO2nanotube arrays were formed on the surface and MN was loaded,the antibacterial activity on periodontal pathogens was stronger.

Designing of a portable arm bracket and its application in PICC placement / 中华护理杂志

Objective To design and apply a portable arm bracket for patients undergoing PICC placement and to solve the problem of raising and placing the arm during PICC placemen and promote comfort level.Methods Ninety-eight patients were provided with portable arm bracket between August and November in 2016 as the experimental group,82 patients were provided with wood board between April and July in 2016 as the control group.The comfort degree,placement success rate,number of multiple attempts to insert needle and advance guidewire,and case of abnormal events were compared between two groups.Results Level 1 comfort in the experimental group was higher than the control group,and level 2,3 and 4 comfort in the experimental group were lower than the control group,and differences were statistically significant (P＜0.001).Placement success rate of the experimental group(98.0％) was slightly higher than that of the control group(93.9％),but the difference was not statistical significant (P=0.31).The number of multiple attempts to insert needle and advance guidewire in the experimental group was lower than the control group,and the difference was statistically significant(P＜0.05).Abnormal events occurred in 3 cases in the experimental group and the incidence rate was 3.1％;there were 6 cases in the control group and the incidence rate was 7.3％.Conclusion The shelve and raising function of the portable arm bracket boost patients' catheter comfort degree,improve the success rate of catheterization and reduce the occurrence of complications cases.

Treatment of Persistent Somatoform Pain Disorder by Floating Needle Therapy and Duloxetine / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To evaluate clinical effect and safety of floating needle therapy and duloxetine in treating patients with persistent somatoform pain disorder (PSPD).</p><p><b>METHODS</b>Totally 108 PSPD patients were randomly assigned to the floating needle treatment group, the duloxetine treatment group, and the placebo treatment group, 36 in each group. Patients in the floating needle treatment group received floating needle therapy and placebo. Those in the duloxetine treatment group received duloxetine and simulated floating needle therapy. Those in the placebo treatment group received the placebo and simulated floating needle therapy. All treatment lasted for six weeks. Efficacy and adverse reactions were evaluated using Simple McGill pain scale (SF-MPQ) and Treatment Emergent Symptom Scale (TESS) before treatment and immediately after treatment, as well as at the end of 1st, 2nd, 4th, and 6th week of treatment, respectively. Hamilton Depression Scale (HAMD, 17 items), Hamilton Anxiety Scale (HAMA) were assessed before treatment and at the end of 1st, 2nd, 4th, and 6th week of treatment, respectively. Patients in the floating needle treatment group and the duloxetine treatment group with the total reducing score rate of SF-MPQ in Pain Rating index (PRI) ≥ 50% after 6 weeks' treatment were involved in the follow-up study.</p><p><b>RESULTS</b>(1) Compared with the same group before treatment, SF-MPQ score, HAMD score and HAMA total scores all decreased in all the three groups at the end of 1st, 2nd, 4th, and 6th week of treatment (P < 0.05, P < 0.01). Besides , each item of SF-MPQ significantly decreased immediately after treatment in the floating needle treatment group (P < 0.01). Compared with the placebo treatment group, SF-MPQ, HAMD, and HAMA total score in the floating needle treatment group significantly decreased after 1, 2, 4, and 6 weeks of treatment (P < 0.05, P < 0.01). SF-MPQ score, HAMD score and HAMA total score in the duloxetine treatment group also significantly decreased after 2, 4, and 6 weeks of treatment (P < 0.05, P < 0.01). (2) There were 3 patients (8.3%) who had adverse reactions in the floating needle treatment group, 17 (50.0%) in the duloxetine treatment group, and 7 (21.2%) in the placebo treatment group. Compared with the placebo treatment group, the incidence of adverse reaction increased in the duloxetine treatment group (χ² = 6.04, P < 0.05). Besides, it was higher in the duloxetine treatment group than in the floating needle treatment group (χ² = 14.9, P < 0.05). (3) There were 19 patients in the floating needle treatment group and 17 patients in the duloxetine treatment group involved in the follow-up study. Compared with 6 weeks after treatment, no significant difference was observed at 3 and 6 months after treatment in the score of SF-MPQ, HAMD, and HAMA in the floating needle treatment group and the duloxetine treatment group. No significant difference was observed between the two groups (P > 0.05). There were 5 patients (29.4%) who had adverse reactions in the duloxetine treatment group, and no adverse reactions were observed in the floating needle treatment group. The adverse reaction rate was significantly different between the two groups (χ² = 4.26, P < 0.05).</p><p><b>CONCLUSIONS</b>Floating needle therapy and duloxetine were effective in treatment of patients with PSPD. However, floating needle therapy could relieve pain more rapidly than duloxetine, with obviously less adverse reactions.</p>

Synergistic effect of RhBMP-2 and bFGF on ectopic osteogenesis in mice

Objective: To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2 (rhBMP-2) and basic fibroblast growth factor (bFGF). Methods: 24 KM male mice were randomly divided into 6 groups with 4 mice in each group, namely, Group A (control group), Group B (only treated with collagen), Group C (treated with 2 ng bFGF+collagen), Group D (treated with 4 μ g rhBMP-2+collagen), Group E (treated with 4 μ g rhBMP-2+2 ng bFGF+collagen) and Group F (treated with 4 μ g rhBMP-2+4 ng bFGF+collagen). The composites were implanted into the intermuscular septum of hind legs mice; whereas in control group, intermuscular septum of mice was separated and no implantation was performed. General observation, detection of concentration of calcium content, micro computed tomography (Micro-CT), three-dimensional reconstruction scan, measurement of bone mineral density (BMD), bone volume fraction (BVF) and trabecular thickness (Tb.Th), as well as histological observation with HE staining and ALP and CD34 immumohistochemical staining were performed. Results: Ectopic osteogenesis was found in Groups D, E and F mice. The difference in concentration of calcium contents was statistically significant between Groups D and E (. P0.05). Micro-CT and three-dimensional reconstruction revealed continuous newborn bone substance in external surface of ectopic bone formation, and the center of bone formation did not show obvious substantial filling by bone substance. The differences in BMD, BVF and Tb.Th were statistically significant between Groups D and E or F (. P<0.01 or <0.05). HE staining showed that in Groups D, E and F, newborn bone substance was mainly located at the edge of ectopic bone formation, and the bone formation in Groups E and F was better than that in Group D. ALP and CD34 immumohistochemical staining revealed the positive expression mainly at the edge of ectopic bone formation, and area of positive expression in Groups E and F was larger than that in Groups D. Conclusions: rhBMP-2 possesses the capacity to induce ectopic osteogenesis independently, but bFGF does not have this ability; the combined application of rhBMP-2 and bFGF can enhance the synergetic effect on inducing ectopic osteogenesis.

Effect of rhBMP-2 sustained-release nanocapsules on the ectopic osteogenesis process in Sprague-Dawley rats

OBJECTIVE@#To explore the effect of sustained-release recombinant human bone morphogenetic protein-2 (rhBMP-2) on ectopic osteogenesis in the muscle pouches of rats through preparing rhBMP-2 sustained-release capsules by wrapping morphogenesis protein bones-2 (BMP-2) using chitosan nanoparticles, and compositing collagen materials.@*METHODS@#Twenty four Sprague-Dawley rats were randomly divided into four groups with six rats in each group, that is Group A (control group), Group B (only treated with collagen), Group C (rhBMP-2+collagen treated group) and Group D (rhBMP-2/cs+collagen treated group). The composite materials for each group were implanted in the bilateral peroneal muscle pouches in rats. The peroneal muscles were only separated without implanting any materials in control group. Rats were sacrificed 2 weeks and 4 weeks post treatment and samples were cut off for general observation, Micro CT scans and histological observation.@*RESULTS@#General observation showed no new bone formation in Groups A and B mice, while new bones were formed in Groups C and D mice. Two weeks after treatment Micro CT scans showed that The bone volume fraction (BVF), trabecular thickness (Tb.Th), bone mineral density (BMD) in Group C mice were all higher than that in Group D (P<0.05). At the fourth week, the BVF, Tb.Th and BMD were significantly higher than that at the second week (P<0.01).@*CONCLUSIONS@#The slow-release effect of rhBMP-2/cs sustained-release capsules can significantly promote ectopic osteogenesis. Its bone formation effect is better than that of rhBMP-2 burst-release group.

Application of ultrasonic inspection in monitoring dynamic healing of mandibular fracture in rabbit model

OBJECTIVE@#To investigate the feasibility of ultrasonic diagnosis for monitoring fracture healing.@*METHODS@#Thirty rabbit models with fraction of mandible body were established by surgically removing partial lower jawbone. At the 1st, 2nd, 4th, 6th, 8th and 12th week after the operation, they were examined by X-ray and ultrasound, respectively. All detection results were scored according to a generally accepted standard. Spearman rank correlation analysis was conducted to explore the relationship between the results of the two inspection methods.@*RESULTS@#In each healing stage, the results of the ultrasonic inspection were basically consistent with those of the X-ray examination, as supported by a Spearman rank correlation coefficient of 0.892 (P<0.001).@*CONCLUSIONS@#Non-invasive ultrasonic inspection can be used instead of X-ray examination to monitor and diagnose fracture healing.