Research progress of oral allergy syndrome / 中华预防医学杂志

Oral allergy syndrome (OAS) is an IgE-mediated hypersensitivity. Patients with pollen allergy will experience oropharyngeal allergy after eating fresh fruits or vegetables containing homologous pathogenesis-related allergen, occasionally accompanied by systemic symptoms, it is a special type of food hypersensitivity in which respiratory allergens and food allergens are similar structurally and lead to the cross-reactivity. At present, there is little research and attention to it in China. To master the definition, epidemiological characteristics, pathological mechanism, diagnosis, prevention and treatment of OAS is very important to the prevention and control of OAS. This article reviews the research progress of OAS, providing reference and prevention basis for clinicians to improve the diagnosis and differential diagnosis of OAS.

Phonetic parameters analysis of articulated consonant with different denture base / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To compare the phonetic parameters changes of titanium denture base materials to that of conventionally processed poly methyl methacrylate (PMMA) resin.</p><p><b>METHODS</b>Forty-two edentulous patients were included in this study. Each patient received two sets of dentures. One of the upper denture was restored with titanium denture base, the other was restored with conventional PMMA denture base. Consonant /x, sh, r, zh, ch, j, q/ associated with place of articulation and manner of articulation were chosen and all of the processed acoustic specimens were stored at computerized speech lab (CSL). Model 4150 (KAY) for the first concentrated frequency area (CFA-1), voice onset time (VOT) and spectrogram were analyzed.</p><p><b>RESULTS</b>The consonant articulation effect of denture base showed significant difference between the two type of dentures in the CSL. The consonant parameters (/x, sh, r, zh, ch, j, q/) CFA-1 of group A [(3595 +/- 102), (3089 +/- 104), (1876 +/- 116), (4078 +/- 116), (3604 +/- 119), (5432 +/- 118), (4934 +/- 121) Hz] was different from the one of group B [(3644 +/- 101), (3280 +/- 88), (1978 +/- 113), (4214 +/- 193), (3817 +/- 49), (5478 +/- 158), (5020 +/- 183) Hz] (P < 0.05). The consonant parameters VOT of /zh, ch, j/ in group A [(54.67 +/- 1.13), (143.80 +/- 2.24), (62.53 +/- 0.75) ms] was different from those of group B [(52.88 +/- 0.34), (137.55 +/- 2.50), (62.00 +/- 1.54) ms] (P < 0.05). The consonant parameters VOT of /g, k/ in group A [(26.94 +/- 0.33), (114.53 +/- 2.15) ms] was different from those of group B [(28.59 +/- 0.65), (124.40 +/- 3.74) ms] (P < 0.05). A redundant spike as it was located prior to the inherent spike could be observed in the /g, k/ spectrogram of the PMMA denture base group.</p><p><b>CONCLUSIONS</b>Titanium denture base is recommended to provide a satisfying form of articulate position with thinner front palate thickness of the denture base. CFA-1 and VOT based CSL are effective acoustic parameters for articulation testing after denture delivery.</p>

Fracture strength of custom-fabricated Celay all-ceramic post and core restored endodontically treated teeth / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The increased use of all-ceramic crown provides a rationale for tooth-colored core. Due to superior mechanical properties, Vita Celay infiltration ceramic developed for crown and bridge works presents the potential for fabricating all-ceramic posts and cores in one piece. This study was conducted to compare the fracture strength of endodontically treated teeth which were thereafter given different types of posts and cores and crowns restoration, respectively. The evaluated post-and-core systems are: custom-fabricated Celay all-ceramic post-core, custom cast metal post-core, and prefabricated stainless steel post (Parapost) with and without 2.0 mm dentine ferrule.</p><p><b>METHODS</b>Sixty freshly extracted human maxillary central incisors were endodontically treated and randomly divided into five groups with 12 samples each. Group A: Celay ceramic post-cores restored teeth with 2.0 mm dentine ferrule. Group B: Celay ceramic post-cores restored teeth with no dentine ferrule. Group C: cast metal post-cores restored teeth with 2.0 mm dentine ferrule. Group D: cast metal post-cores restored teeth with no dentine ferrule. Group E: prefabricated post and composite cores restored teeth with 2.0 mm dentine ferrule. All teeth were restored with Celay ceramic crowns. Each specimen was subjected to a load at a 45-degree angle to the long axis on MTS 810 material testing machine until failure, at crosshead speed of 0.02 cm/minute. Analysis of variance followed by the Newman-Keuls pairwise multiple comparison tests were used to compare the results of the groups tested.</p><p><b>RESULTS</b>There was a statistically significant difference among the five groups (P < 0.01). Celay ceramic post-cores restored teeth with 2.0 mm dentine ferrule [(758.35+/-19.26) N] and cast metal post-cores restored teeth with 2.0 mm dentine ferrule [(756.63+/-66.22) N] had a significantly greater mean fracture strength than the other three groups in which no significant difference was observed. The 2.0 mm dentine ferrule could cause significant fracture resistance alteration of Celay post-core restored teeth.</p><p><b>CONCLUSIONS</b>When covered with Celay ceramic crowns, Celay post-cores restored teeth with 2.0 mm dentine ferrule and cast metal post-cores restored teeth with 2.0 mm dentine ferrule have similar fracture strength. There was a statistically significant difference between the fracture resistance of Celay post-core restored teeth with and without 2.0 mm dentine ferrule.</p>

Cloning and expression of the succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum Chinese strain in E. coli / 中南大学学报(医学版)

OBJECTIVE@#To clone the full-length gene encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) Chinese strain and express it in Escherichia coli.@*METHODS@#According to the published incomplete EST (BU804141) of SjSDISP and the sequence of multiclone sites of lambda gt11 vector, 2 pairs of primers were designed and synthesized. Then the 3' and 5'ends of the EST of the SjSDISP from adult Schistosoma japonicum cDNA library were amplified by anchored PCR. After sequencing, a full-length cDNA sequence of the SjSDISP was obtained, and then it was cloned into prokaryotic expression vector pGEX-4T-1. Identified by agarosed gel electrophoresis, endonucleases digestion and PCR, the resultant recombinant plasmid was used for the expression under the temperature-dependent condition and Western blot analysis.@*RESULTS@#A 1,071 bp sequence was obtained. Sequence analysis showed that the fragment contained a complete open reading frame (ORF), encoding 278 amino acid residues. This target fragment was cloned into the prokaryotic expression vector pGEX-4T-1, and expressed in Escherichia coli. SDS-PAGE revealed that the molecular weight of the expressed fusion recombinant product was 56 kD. Western blot showed that the recombinant protein was recognized by polyclonal rabbit antiserum immunized with Schistosoma japonicum adult worm antigen.@*CONCLUSION@#Cloning of the full-length gene encoding SjSDISP and its bacterial expression were successfully done.