ABSTRACT
Squalene synthase (SQS) is a key enzyme in plant terpenoid biosynthetic pathway. This study focused on cloning and analysis of Huperzia serrata SQS (HsSQS1) gene. After searching the transcriptome dataset of H serrata, one unique sequence encoding SQS was discovered. The primers were designed according to the transcript sequence of HsSQS1 from the H. serrata transcriptome dataset. The open reading frame of HsSQS1 was cloned using RT-PCR strategy. The bioinformatic analysis of this gene and its corresponding protein were performed. The cDNA (named as HsSQS1) contains a 1263 bp open reading frame and encodes a predicted protein of 420 amino acids. The GenBank accession number for this gene is JQ004938. HsSQS1 contains two transmembrane regions, without signal peptide. The conserved domain of squalene synthase was presented in HsSQS1. HsSQS1 was more abundant in H. serrata root than in leaf and stem. This study cloned and analyzed squalene synthase gene from H. serrata for the first time. The result will provide a foundation for exploring the mechanism ofterpenoid biosynthesis in H. serrata plants.
Subject(s)
Amino Acid Sequence , Biosynthetic Pathways , Cloning, Molecular , DNA, Complementary , Genetics , Expressed Sequence Tags , Farnesyl-Diphosphate Farnesyltransferase , Genetics , Metabolism , Genes, Plant , Genetics , Huperzia , Genetics , Molecular Sequence Data , Open Reading Frames , Phylogeny , Plant Leaves , Plant Roots , Plant Stems , Plants, Medicinal , Genetics , Triterpenes , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents in the rhizome of Elaeagnus bockii.</p><p><b>METHOD</b>Compounds were isolated by silica gel and Sephadex LH-20 chromatography. Their structures were elucidated by means of spectral analysis.</p><p><b>RESULT</b>Seven compounds were isolated from the rhizomes of E. bockii, and their structures were identified as angelicin (1), psoralen (2), vanillic acid (3), bakuchiol (4), oleanolic acid (5), ursolic acid (6) and beta-sitosterol (7 ).</p><p><b>CONCLUSION</b>All these compounds were obtained from E. bockii for the first time.</p>