ABSTRACT
Es inducing human spleen cells in 30 cases were studied. We found that Es could not only induce IFN-? from human spleen cells, but also IL-2 and LT. The mixed lymphokines containing IFN-?(3269.6?1634.4 U/ml), IL-2 (64.8?40.8 U/ml) and LT (54.4?44.6 U/ml) were induced by ES. Our study shows that the mixed lymphokines appeared to be cytotoxic to human SMMC-7721, SPC-3, HcLa, Jutkat arid Molt-4 cells lines at all levels, but it had no cytotoxic effect to normal cells (WISH cells).
ABSTRACT
Interleukin-6 (IL-6) is a pleiotropic cytokine which has antitumor activity. In the present study, a model for fibroblasts-mediated IL-6 gene therapy and its immune regulation are described. Human IL-6 cDNA was inserted into plasmid vector BCMGNeo containing a neomycin resistance gene. BCMGNeo-IL-6 was transferred into NIH3T3 fibroblasts by calcium phosphate coprecipitation method. A fibroblast clone (T6.6) secreting 1L-6 at highest level was selected by G418 resistance selection and limiting dilution. When T6.6 was implanted i.p. into mice, IL-6 could be detected in serum after 12 h. Even after 96 h, serum IL-6 still maintained at high level. Lymphocyte proliferation and IL-2 production could be enhanced significantly after in vivo implantation of T6.6. These results demonstrate that fibroblasts -mediated IL-6 gene therapy could augment immune function efficiently and outline a novel strategy for cancer treatment.
ABSTRACT
In this study, 5'-CMP was sulfonated, and then the modified 5'-CMP was connected to a protein carrier as an immunogen to immunize BALB/c mice. After cell hybridization, screening and recloning , a McAb (B10) with high sensitivity and specificity was selected. In a dot Hot using the McAb B10, less than 0.05 pg of sulfonated DNA could be detected while 10 ng of DNA was not coloured The result showed that the sensitivity of McAb B10 was higher than that of the McAb from "Chemiprobe" kit
ABSTRACT
The efficacy of different inducers used to induce immune interferon (IFN-?)from ionsillar cells of onsillectomized normal children was sfudied.Tonsillar cells were made in suspension of 1?107 cells/ml treated with antibiotics,and cultured for 2-3 days and then supernants were assayed for IFN-r.The results show that cells of individual human tonsil could produce IFN-? spo ntaneously owing to the induction by pharyngeal normal flora.Esculentoside (Es) alone or .pokeweed mitogen (PWM) alone,their combinatian or with other inducers were capable of inducing IFN-?.Among them,PWM was more effective in inducing IFN-? than Es,and its efficacy was similar to that of PWM+ scoppolamine,but higher than that of PWM+C.parvum or PWM+Con A.Mixed lymphocyte culture (MLC) of human tonsils,with or without PWM or Es,could produce IFN-?,but slightly higher titer was obtained with MLC+PWM.The possible reasons for the difference of efficacy of various methods in inducing IFN-? from human tonsillar cells are discussed.