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Objective To compare the discrepancies among results of three commonly used laboratory direct test methods for maximal oxygen uptake(VO2max),explore their linear regression relationships,mutual predictability and comparability.Methods Using a quasi-experimental design of cluster sampling and within-group interaction design,20 male cross-country skiers were tested for VO2max using the Bruce protocol(Method 1),90-second incremental load exercise on power bicycle(Method 2),and 1-minute incremental load exercise on treadmill(Method 3),with an interval of one week.The indepen-dent and dependent variable were the three VO2max test methods and the VO2max,respectively.Results Significant differences were found in the average VO2max of the three test results,with the value mea-sured by Method 1 ranking the first,followed by that assessed by Method 3 and Method 2(P<0.05).Moreover,the frequency of individual differences in the results of the three methods showed that the VO2max of Method 1 was about 6 and 3 ml/min·kg higher than that measured by Method 2 and 3.However,at the same treadmill speed,the average blood lactate evaluated using Method 3 was higher than Method 1,and the speed reached aerobic and anaerobic thresholds about one speed unit(1 km/h)lower than Method 1.Meanwhile,linear regression analyses of the test results between Method 1 and 2,as well as Method 1 and 3 showed that both the regression models and coefficients were statis-tically significant(P<0.001),with the R-squared values of 9.25 and 9.05,respectively.Conclusion The Bruce protocol performs best in assessing the maximum value of the athlete's VO2max phase,whose results have linear regression relationships with the other two methods,and can be used for pre-dicting their results.Moreover,athletes of different events and levels can choose different VO2max test methods accordingly.Lastly,the speed and heart rate ranges corresponding to the aerobic and anaero-bic thresholds can serve as an effective and convenient method to control the training intensity.
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Hongkong pediatric specialist training had successful experiences in the last twenty years.Hongkong hospital authority and Hongkong college of pediatricians managed pediatric specialist training together and made a series of regulations,which have strict training rotation requirements.Training hospitals all need to obtain the authentication including basic training,higher training and overseas training agencies.After 6 years strict training,the trainees have strong pediatric basic theories,procedure abilities,evidence-based practice and team work spirit.In short,the experiences of Hongkong pediatric specialist training is deserved to be learned by the standard training of pediatric resident in mainland China.
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This study retrospectively reviewed the experimental clinical programs at The First Affiliated Hospital of Nanjing Medical University. Experience was summarized, and methods for improving clinical pharmaceutical research were discussed including recruiting talent people, managing resources and trail results, and establishing international cooperations.
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Forty-three children with congenital hypothyroidism(CH)underwent 99mTc thyroid scintigraphy, after being followed up by receiving levothyroxine till 2 to 3 years of age. The results showed that thyroid agenesia happened in 37 cases( 86.05% ) while entopic gland in 6 cases (13.95% ). Thyroid scintigraphy with 99mTc is an informative procedure in determining etiology and treatment schedules for children with CH.
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Objective To observe he therapeutic effect of Zhuanggu guanjie pill combined with Yunnan hongyao capsule on acute ankle sprain.Methods 67 cases with acute ankle sprain were randomly recruited into an observation group and a control group.The observation group was orally administrated with 6g Zhuanggu guanjie pill,twice daily,and 0.5g Yunnan hongyao capsule,thrice daily.The control group was orally administrated with 6g Zhonghua dieda pill,twice daily.The therapeutic effect,changes of pain score,and healing time were observed in both groups.Results The effective rate was 97.4%in the observation group and 75.86%in the control group.There was statistical difference between the two groups(P<0.05).Pain scores were 3.92±0.49 and 1.00±0.92 in the treatment group before and after the treatment,as compared to 3.69±1.07 and 2.1 7±1.66 respectively in the control group.There was statistical difference in both in-group comparison before and after the treatment and between-group comparison after the treatment.The healing time were(4.62±1.92)d and (17.2±0.53)d in the treatment group and the control group respectively,also showing a significant difference(P<0.01).Conclusion Zhuanggu guanjie pill combined with Yunnan hongyao capsule is an effective method for treating acute ankle sprain,and shortening healing time.
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With the aid of scientific research management information system, we established an in-formation management for the scientific research funds. The structure,function and application of the system were introduced in this paper.
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This article presented the idea of implementation for the general hospital scientific research management information system.Based on the network and database,the scientific research management information system was constructed by analysis on the operation process of it;the information management of the scientific research management was implemented.This modern scientific research management information would improve the hospital scientific research management level.
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<p><b>OBJECTIVE</b>To establish an effective laboratory examination system for carrier detection and prenatal diagnosis of haemophilia Alpha(HA) with a variety of molecular biological methods which are simple,rapid and easy to use.</p><p><b>METHODS</b>Detection of inversion involving intron 22 in the FVIII gene was completed by long distance polymerase chain reaction (PCR) and linkage analysis was performed by using several genetic polymorphisms including an intragenic Bcl I RFLP, 2 STRs and an extragenic St14 VNTR.</p><p><b>RESULTS</b>Intron 22 inversion was observed in 10 out of the 21 (47.6%) pedigrees examined. Prenatal diagnosis was completed in 3 pedigrees. A further combination of the four intragenic and extragenic polymorphic loci gave an informative rate of 94.7%.</p><p><b>CONCLUSIONS</b>Female relatives in HA families with inversion can be detected with direct diagnostic procedure. The application of long distance PCR makes the detection much more simple and rapid. For families without inversions,it is easier and more cost-effective to undertake linkage analysis of genetic polymorphism based on PCR.</p>
Subject(s)
Female , Humans , Pregnancy , Chromosome Inversion , Genetic Carrier Screening , Hemophilia A , Diagnosis , Genetics , Introns , Minisatellite Repeats , Polymorphism, Restriction Fragment Length , Prenatal DiagnosisABSTRACT
OBJECTIVE To find out the prevalent distribution and multidrug resistance trend of meticillin-resistant Staphylococcus(MRS) and to prevent fulminant prevalence of MRS and opt for effective therapeutic means.METHODS The bacterium was(identified) by the way of API Staph and the TH-16S′s coding tube.The(antimicrobial) susceptibility testing was adoped by ATB-STAPH5 and MRS was examined by dilution and K-B.All statistical analyses were performed using SPLM 3.0 software.RESULTS The isolation rate of meticillin-resistant S.aureus(MRSA) and meticillin-resistant coagulase negative Staphylococcus(MRCNS) was 38.09%,20.00%and 87.65%,89.00%,(respectively) in 2 years.Along with the age of patients,the infection from MRS was(increasing).The isolation rate of MRSA was 28-26%,but that of MRCNS was more than 80% from S.epidermidis,S.haemolyticus,S.hominis,and S.saprophyticus subsp saprophyticus.All parts of our body can be(infected) by MRS.The more than 30% MRSA were multidrug resistant and the approximately 11.87-13.75% MRCNS were also multidrug(resistant).(CONCLUSIONS) The isolation rate of MRSA from national surveillance(network) is not(different) with that of MRCNS.