ABSTRACT
Cholestatic hepatopathy is one of the most common diseases in infants including the newborn and it is one of the most significant reasons for infantile death and disability. There are a large number of causes and a large variance in prognosis of this disease. This paper introduces approaches to the diagnosis of biliary atresia and cholestatic hepatopathy and presents a synopsis of therapies for cholestatic hepatopathy in infants nowadays. This paper points out that in future, cooperation across disciplines should be enhanced; new technologies should be developed; academic communication should be held regularly; the early diagnosis and personalized medication of cholestatic hepatopathy in infants should be improved.
ABSTRACT
Aim To study the influences of miR-203 on ursolic acid (UA ) sensitivity in leukemic K562 cell.Methods In the experimental system,eukaryot-ic expression vector of hsa-miR-203 (PmiR-203 )was transfected into K562 cells using LipofectamineTM 2000.K562 cells were incubated with different con-centration of UA alone or in combination with PmiR-203 .The cell proliferation was analyzed by MTT as-say.The cell apoptosis was measured by double stai-ning with Annexin V and Propidium Iodide.The ex-pression of Bcr/abl protein was detected by Western Blot.Results The miR-203 promoted the sensitivity of UA by up to 1 .55-fold and the IC50 was reduced from 44.3μmol · L-1 to 30.7 μmol · L-1 .The a-mount of apoptotic cells was increased in UA combined with PmiR-203 group (P<0.05).PmiR-203 downreg-ulated the expression of Bcr/abl protein in K562 cells. Conclusion Our results support a substantial role of miR-203 that enhances UA sensitivity in K562 cell and the mechanism appears to be related to the dounregula-tion of Bcr/abl by miR-203 .
ABSTRACT
Objective To investigate the clinical effect ofQiju-Dihuang decoction combined with tears naturale(hypromellose 2910,dextran 70 and glycerol eye drops)on dry eye of liver and kidneyYin deficiency patterns.Methods Sixty patients with dry eye in Fangxian People’s Hospital and Fangxian Hospital of TCM from Jan. 2010 to Aug. 2013 were divided into a control group(30 patients)and a treatment group(30 patients) randomly according to registration order. The patients in the control group were treated with tears naturale, while patients in the treatment group were additionally treated withQiju-Dihuang decoction orally on the basis of the control group. Both groups were treated for 8 weeks. After treatment, the clinical symptoms and signs were compared and the changes of breakup time of tear film(BUT), Schirmer test I(S1T)and corneal fluorescein staining(FL)score were measured in both groups.Results The total effective rates in the treatment group and in the control group were 73.33%(22/30)and 46.67%(14/30), respectively. The difference was statistically significant(χ2=4.44,P<0.05). After treatment, the BUT, S1T and FL score of the treatment group and the control group was significantly improved than those before in both groups(t=4.200, 4.374, 9.098, 2.147, 6.627,P<0.05 orP<0.01), and the degrees of BUT, S1T score increased and FL score decreased in the treatment group were superior to those in the control group(t=2.75, 2.43, 3.05,P<0.05 orP<0.01). ConclusionsQiju-Dihuang decoction combined with tears naturale can obviously improve dry eye of liver and kidneyYin deficiency patterns, increase tear secretion, and reduce the damage of canthus and conjunctiva. Its curative effect was superior to simple tears naturale.