ABSTRACT
Objective To investigate the possible mechanism of scutellarin on the apoptosis of human tongue squa?mous carcinoma Tca8113 cells. Methods Human tongue squamous carcinoma Tca8113 cells were divided into control group and scutellarin groups (80, 120 and 160 mg/L). Tunel method was used to detect the apoptosis. Immunohistochemistry and Western blot assay was used to detect the caspase-8 protein expression of cells. Results (1)The apoptotic rates of Tca8113 cells were significantly high in scutellarin groups (80, 120 and 160 mg/L) than those in control group [(17.63 ± 0.25)%, (36.23±0.36)%, (51.84±0.58)%vs (4.45±0.27)%, P<0.05].(2)Compared with control group, the expressions of cas?pase-8 protein were significantly increased in different concentrations (80, 120 and 160 mg/L) of scutellarin groups (0.283± 0.040 vs 0.474±0.031, 0.592±0.077, 0.781±0.020,P<0.05). Conclusion Scutellarin could obviously induce the apoptosis of human tongue squamous carcinoma Tca8113 cells, which may be related to the caspase-8 protein expression.
ABSTRACT
Objective:To investigate the effects of scutellarin on Bcl-2 and Bax expression in human tongue squamous carcinoma Tca-8113 cell.Methods:Tca8113 cells were treated with 80,120 and 160μg/ml scutellarin for 48 h respectively.Immunocytochem-istry method and RT-PCR were applied to observe the expression of Bcl-2 and Bax protein and mRNA in the cells.Results:With the increase of scutellarin concentration,Bcl-2 protein and mRNA decreased(P<0.05),Bax protein and mRNA increased(P<0.05). Conclusion:Scutellarin may downregulate Bcl-2 expression and upregulate Bax expression in Tca-8113 cells.
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Objective To investigate the morphological changes and expression of nerve growth factor(NGF) in submandibular gland of C57BL/ksj\|db/db mutant diabetic mice. Methods Animals were C57BL/ksj\|db/+m mutant diabetic mice which were imported from Japan.The pure Zygote,that is db/db mouse,displays obesity and high blood glucose over 10mmol/L.The test groups were 3,4,6,8,10 month\|old db/db mice and the age\|matched db/+m mice as control.Immunohistochemistry technology was applied in this study. Results In db/db diabetic mice,submandibular gland atrophied and took on the significant morphological changes in acinar,duct and granular convoluted tubule.The NGF Immunopositive cells in submandibular gland were significantly less than the age\|matched control animals and showed a descend tendency with the progression of diabetes millitus.Conclusion\ The decreased expression of NGF demonstrates NGF synthesis and secretion in granular convoluted tubule of submandibular gland was decreased.The lower NGF levels may be associated with development of diabetic neuropathy.\;[