ABSTRACT
AIM: To establish a simple and rapid method for the quality control of Chinese traditional medicine. METHODS: Pericarpium Citri Reticulatae,Fructus Psoraleae,Rhizoma Atractylodis macrocephalae and Radix Codonopsis in Wenweishu Tablets were identified by TLC at two thin-layer chromatographic plates.The contents for hesperidin,psoralen and isopsoralen in this preparation were determined simultaneously by HPLC. RESULTS: The methodological study showd that a good linear correlation existed in the range of 0.039 6-0.495 ?g(r=(0.999 99)) of hesperidin,0.018 5-0.231 ?g(r=0.999 98) of psoralen and 0.019 7-0.246 2 ?g(r=0.999 91) of isopsoralen applied respectively.The average recovery of hesperidin was 98.42%(n=9),RSD was 2.69%;The average recovery of psoralen was 98.29%(n=9),RSD was 1.08%.The average recovery of isopsoralen was(98.93%)(n=9),RSD was 2.41%. CONCLUSION: The method is simple、rapid and practicle.