ABSTRACT
Objective To investigate the effect of metformin on the proliferation and apoptosis in human pancreatic cancer cell line CFPAC-1,and to explore the potential mechanism.Methods Human pancreatic cancer CFPAC-1 cells were cultured in vitro,and were treated with metformin at different concentrations (1,2.5,5,10,20,40,60 mmol/L) for different durations (24 h,48 h and 72 h),and cells without treatment were considered as control group.Cell proliferation was evaluated by CCK-8,cell cycle was determined by flow cytometry,apoptosis was determined by Annexin V/PI double staining method,and Western blot was used to detect the protein expression of p-AMPK,FAS,cyclin D1,Bcl-xl,Bax,caspase-3 and survivin.Results Metformin could inhibit the proliferation of CFPAC-1 cells in a time and dose dependent manner.Forty-eight hours after 40 mmol/L metformin treatment,the proportion of CFPAC-1 cells in phase G0/G1 was significantly increased [(65.93 ± 0.27)％ vs (42.89± 1.02)％],and the proportion of CFPAC-1 cells in phase G2/M,S was significantly decreased [(22.01 ± 2.95) ％ vs (38.28 ± 4.93) ％,(13.58±0.43)％ vs (20.12 ± 3.38)％],and the difference between the two groups was statistically significant (P ＜0.05).The apoptosis rate was increased from (3.01 ± 0.49) ％ to (32.97 ± 3.19) ％,(P ＜ 0.05) ; and the expression of p-AMPK,Bax,and caspase-3 was increased,while the expression of FAS,cyclin D1,Bcl-xl,survivin were decreased.Conclusions Metformin can inhibit proliferation and promote apoptosis of CFPAC-1 cells mainly by activation of AMPK pathway,and down-regulation of FAS,cyclin D1,survivin and Bcl xl/Bax ratio,as well as up-regulation of caspase-3.
ABSTRACT
Objective To investigate the clinical features of infantile diarrhea with intussusception,improve the level of diagnosis and treatment,reduce the misdiagnosis and delayed treatment.Methods The clinical data of 23 cases with infantile intussusception were retrospectively analyzed.Results Twenty-three cases of children with diarrhea symptoms at the beginning of the disease had different degree,12 cases characterized by vomiting,diarrhea,fever and other symptoms of acute gastroenteritis,5 cases stool were mucous pus blood,6 cases for blood in the stool.Five cases were confirmed within 24 h of the diagnosis (3 cases with air enema reduction of intussusception,2 cases with manual reduction),11 cases were confirmed at 24 h to 3 d of the diagnosis and operated,7 cases were confirmed more than 3 d of the diagnosis and operated.Except 1 cases was died from shock,22 cases were cured.Conclusion Infantile intussusception atypical symptoms,illness development is rapid,in critical condition,easy misdiagnosis,missed diagnosis,should actively improve the standard of diagnosis.,early diagnosis,so as not to delay treatment.