ABSTRACT
Objective:To investigate the protective effect of Angelica polysaccharide on the glaucoma-induced retinal neuronal injury,and to elucidate its mechanism.Methods:Eighty-four SD rats were randomly divided into control group,model group,positive drug group,low,middle and high doses of Angelica polysaccharide groups.The model of rat glaucoma was established by sclera sclerotherapy.The intraocular pressures (IOP) of rats in each group were measured.The superoxide dismutase (SOD) activities,malondialdehyde (MDA) contents and nitric oxide (NO) levels in retina tissue of the rats were measured by colorimetric assay.The expression levels of Caspase-3 mRNA and protein in retina tissue of the rats were observed by RT-PCR and Western blotting method.Results:Compared with control group,the IOP of the rats in model group at each time point were significantly increased (P＜0.05);compared with model group,the IOP of the rats in different doses of Angelia polysaccharide groups were significantly decreased (P＜0.05);compared with low dose of Angelica polysaccharide group,theIOP of the rats in high dose of Angelica polysaccharide group was significant decteased (P＜0.05).The levels of MDA and NO in the retina tissue of the rats in model group were higher than those in control group (P＜0.05),but the SOD activities were decreased (P＜0.05);compared with model group,the levels of MDA and NO in retinal tissue of the rats in different doses of Angelica polysaccharide group were significantly decreased (P＜0.05),and the SOD activities were significantly increased (P＜0.05);compared with low dose of Angelica polysaccharide group,the above indexed had significant differences in high dose of Angelica polysaccharide group (P＜0.05).Compared with model group,the expression levels of Caspase-3 mRNA and protein in different doses of Angelica polysaccharide groups were significantly decreased (P＜0.05);compared with low dose of Angelica polysaccharide group,the expression levels of Caspase-3 mRNA and protein were significantly decreased (P ＜ 0.05).Conclusion:Angelica polysaccharide has protective effect on the retinal tissue of glaucoma model rats,which can decrease the levels of MDA and NO in retina tissue and increase the activity of SOD and decrease the expression levels of Caspase-3 mRNA and protein in retina tissue.It may be one of the mechanisms of polysaccharide protecting in retinal tissue nerve cells.
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BACKGROUND:Stem cel transplantation is a new method for blinding eye disease. But there is a lack of research about the protective effect of retinal stem cel transplantation on retinal ganglion cel s in glaucoma. OBJECTIVE:To explore the protective effect of retinal stem cel transplantation on retinal ganglion cel s of rats with glaucoma. METHODS:Forty-five Sprague-Dawley rats were randomly divided into three groups (n=15 per group) including control, model and retinal stem cel transplantation groups. Rat models of glaucoma were prepared in the latter two groups, and at 7 days after modeling, rats in the three groups were given intravitreal injection of 1 mL retinal stem cel s (5x106 cel s), the same amount of PBS, and no treatment, respectively. Subsequently, relative indicators were detected at 2 weeks after transplantation. RESULTS AND CONCLUSION:The expressions of brain-derived neurotrophic factor and insulin-like growth factor I protein as wel as the number of retinal ganglion cel s were the highest in the control group, fol owed by the retinal stem cel transplantation group model group, and the lowest in the model group (P<0.05). The number of apoptotic retinal ganglion cel s in model group was significantly higher than that of control group (P<0.05), and which in the retinal stem cel transplantation group was significantly lower than that in the model group (P<0.05), but higher than that in the control group (P<0.05). These results suggest that retinal stem cel transplantation for rat glaucoma can exert a protective effect on retinal ganglion cel s.
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Objective To determine pharmacokinetics profiles of sodium alginate dexamethasone in perilymph after trans-round window administration of the sodium alginate dexamethasone gel in vivo.Methods 30 healthy guinea pig were randomly divided into 6 groups.Alg-Dex gel was placed into the niche of the round window of the guinea pig on the right ear.The perilymphetic samples were harvested on day 1,2,3,4 and 5 after administration of Alg-Dex gel respectively.The concentrations of dexamethasone in perilymph were assayed with high performance liquid chromatograph(HPLC).Results Concentrations of dexamethasone in perilymph on day 1,2,3,4 and 5 after administration of Alg-Dex gel were 0.49?0.06,1.32?0.28,0.65?0.08,0.66?0.05,0.53?0.17 mg/L respectively.Concentrations of dexamethasone in perilymph reached a peak on the second day and maintained 0.61?0.07 mg/L steadily on following days after administration.Conclusion Dexamethasone can be released from the sodium alginate dexamethasone gel persisitently and steadily in vivo.