Fetal RHD screening from circulating cell-free DNA in RhD-negative pregnant women / 中华围产医学杂志

Objective:To investigate the RHD genotypes of RhD-negative pregnant women and explore the optimum strategy for fetal RHD screening among this population in the region. Methods:This prospective study recruited 33 cases of RhD-negative singleton pregnancies at ≥12 weeks of gestation in Nanjing Drum Tower Hospital from March to November 2021. On the basis of RHD genotyping, quantitative real-time polymerase chain reaction (PCR) was used to amplify the exons 5 and 10 of RHD gene in the circulating cell-free DNA of RhD-negative pregnant women harboring whole RHD gene deletion and RHD-CE(2-9)- D. High-throughput sequencing was performed to detect chr1:25648453 locus from circulating cell-free DNA in plasma of RhD-negative pregnant women harboring RHD 1227A mutation to screen the fetal RhD blood group. Neonatal umbilical cord blood samples were collected for verifying fetal RHD genotyping. Descriptive statistical analysis was used. Results:Whole RHD gene deletion homozygous genotype ( n=20, 60.6%), RHD-CE(2-9) -D/whole RHD gene deletion heterozygous genotype ( n=5, 21.2%), RHD 1227A/whole RHD gene deletion heterozygous genotype ( n=7, 15.2%) and RHD 711delC/whole RHD gene deletion heterozygous genotype ( n=1) were identified in the 33 RhD-negative pregnant women. In the 25 cases with whole RHD gene deletion homozygous genotype or RHD-CE(2-9)- D/whole RHD gene deletion heterozygous genotype, 22 fetuses were RhD-positive and three were RhD-negative based on prenatal screening, which were confirmed by the neonatal serological test results after birth. In the seven cases carrying RHD 1227A/whole RHD gene deletion heterozygous genotype, all fetuses were RhD-positive, which were consistent with the results of serological detection after delivery. The case harboring RHD 711delC/whole RHD gene deletion heterozygous genotype did not receive fetal RHD screening. Conclusions:This study suggests that whole RHD gene deletion homozygous genotype is the most common allele in RhD-negative population in this area, followed by RHD 1227A/whole RHD gene deletion heterozygous genotype and RHD- CE(2-9)- D/whole RHD gene deletion heterozygous genotype. For women with whole RHD gene deletion homozygous genotype, RHD- CE(2-9)- D, or RHD 1227A mutation, fetal RHD screening with quantitative real-time PCR and high-throughput sequencing are important for the management of RhD-negative pregnant women.

The predictive value of plasma Glypican-4 levels in the prognosis of children with pneumonia and acute respiratory distress syndrome / 中国小儿急救医学

Objective:To investigate the effect of plasma Glypican-4 level on the prognosis of children with pneumonia and acute respiratory distress syndrome(ARDS) and its predictive value.Methods:From April 2018 to April 2021, 92 children with pneumonia and ARDS admitted to the PICU of Baoding First Hospital for the first time were selected as the pneumonia+ ARDS group, 87 children with pneumonia admitted to the Pediatrics Department of this hospital were selected as the pneumonia group, and 95 children for physical examination in the same hospital were selected as control group.The pediatric critical illness score(PCIS)was used to score the children in pneumonia+ ARDS group and pneumonia group.The enzyme-linked immunosorbent assay(ELISA)method was used to detect the levels of Glypican-4 in plasma of three groups.Receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of plasma Glypican-4 in pneumonia complicated with ARDS, and the prognosis-related factors was analyzed by the Cox risk regression model.Results:The heart rate, pneumonia history, 28-day mortality rate, and plasma Glypican-4 in the pneumonia+ ARDS group were significantly higher than those in control group and pneumonia group, and PCIS score in pneumonia+ ARDS group was lower than that in pneumonia group ( P<0.05). According to the prognosis of pneumonia+ ARDS group on 28 days, the children were divided into survival group (74 cases) and death group (18 cases). Compared with the survival group[(3.92±0.31)μg/L], the plasma Glypican-4 level in death group[(4.78±0.35)μg/L] increased, whose difference was statistically significant( t=10.292, P<0.001). ROC curve analysis results showed that the area under the ROC curve of Glypican-4 predicting the prognosis of children with pneumonia combined with ARDS was 0.92, whose sensitivity was 94.2% and specificity was 88.3%, as well as the cut-off value was 4.11 μg/L.Multivariate Cox analysis showed that Glypican-4 level(high expression), heart rate (high frequency) and the history of pneumonia were prognostic risk factors affecting the prognosis of children with pneumonia and ARDS( P<0.05). Conclusion:The level of plasma Glypican-4 in children with pneumonia and ARDS significantly increased, and it is closely related to the prognosis of children with pneumonia and ARDS, and has high predictive value for the prognosis of children with pneumonia and ARDS.

Optimization of Primary Processing Technology of Gentiana rigescens by Box-Behnken Design-response Surface Method / 中国药房

OBJECTIVE：To optimize the primary processing technology of Gentiana rigesce ns. METHODS ：HPLC method was adopted for content determination of loganic acid ，swertiamarin and gentiopicroside in G. rigescens ，and overall desirability value （OD value ） of the contents of above 3 components was taken as index to carry out single factor test on blanching temperature，blanching time and drying temperature. Based on that ，Box-Behnken design-response surface methodology was used to optimize primary processing technology of G. rigescens . Validation test was also performed. The samples prepared by optimized technology were compared with those dried in the shade. RESULTS ：The optimal primary processing technology of G. rigescens included blanching time of 5 min，blanching temperature of 40 ℃ and drying temperature of 60 ℃. Validation test showed that the average OD value of the 3 components was 0.565 2，with a deviation of 0.94％ from the predicted value （0.570 6）. Compared with samples dried in the shade ，OD value of 3 components in samples prepared by optimized technology were increased significantly ， indicating the quality of the samples prepared by the optimized technology was better. CONCLUSIONS ：The optimal technology is stable and feasible ，and can be used for the primary processing of G. rigescens .

Effect of alendronate combined with salmon calcitonin in the treatment of elderly patients with hip osteoporotic fracture / 中国基层医药

Objective:To investigate the effect of alendronate sodium tablets combined with salmon calcitonin in the treatment of elderly patients with hip osteoporotic fracture, and the changes of serum bone metabolism.Methods:From January 2016 to December 2019, 86 elderly patients with osteoporotic hip fracture who were operated in Xin'an International Hospital were divided into observation group (43 cases) and control group (43 cases) according to the random digital table method.The control group was treated with salmon calcitonin, and the observation group was treated with alendronate sodium on the basis of the control group.The course of treatment was 6 months in both two groups.The changes of serum calcium and phosphorus, Harris score of hip joint and bone metabolism were compared before and after treatment.Results:There were no statistically significant differences in serum calcium and phosphorus levels between the observation group and the control group (all P>0.05). After treatment, the Harris score of hip joint in the observation group[(87.10±3.46)points] was higher than that in the control group [(78.65±4.32)points]( t=10.011, P<0.05). The serum β-CTX [(0.27±0.05)μg/L] in the observation group was lower than that in the control group [(0.39±0.07)μg/L], while the ALP [(117.93±10.15)U/L] in the observation group was higher than that in the control group[(98.32±8.97)U/L], the differences were statistically significant( t=9.147, 9.493, all P<0.05). Conclusion:Alendronate combined with salmon calcitonin can improve the bone metabolism of elderly patients with hip osteoporotic fracture, and improve the function of hip joint.

Prior HIV testing and determinants among student men who have sex with men in Guangzhou / 中国学校卫生

Objective@#To understand prevalence and determinants of prior HIV testing among male students who have sex with men (MSM) in Guangzhou,and to provide a reference for improving their HIV set.@*Methods@#Students who were MSM were recruited in Guangzhou from May 2017 to April 2018. Data on socio-demographic characteristics, sexual behavior, AIDS prevention service access and HIV testing history were collected. Multivariate non-conditional logistic regression was conducted to explore determinants with HIV testing.@*Results@#The median age of 223 students who were MSM was 22 years old (interquartile: 20-23). About 65.47% (146/223) of them had a history of HIV testing. Multivariate non-conditional logistic regression showed individuals who sought sexual partners online (OR=3.24, 95%CI=1.32-7.98), had anal sex in the last 6 months (OR=2.73, 95%CI=1.26-5.93), and had received AIDS prevention services(OR=2.87, 95%CI=1.51-5.34) were more likely to have a history of HIV testing. @*Conclusion@#Prior HIV testing among student MSM in Guangzhou was relatively low. Intervention should be tailored targeting student MSM who seek sexual partners offline, have only oral sex in the past 6 months, and receive no AIDS prevention services in the past year to expand the coverage of HIV testing.

Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli / 生物工程学报

Poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] belongs to the polyhydroxyalkanoates (PHA) family and possesses promising properties including biocompatibility and biodegradability. In this study, we directly synthesized P(3HB-co-LA) with glucose by introducing the β-ketothiolase and acetoacetyl-CoA reductase from Ralstonia eutropha, the engineered propionate CoA transferase from Clostridium propionicum and the engineered polyhydroxyalkanoate synthase from Pseudomonas fluorescens strain 2P24 into Escherichia coli. The polymer content was 83.9% (W/W), and the molar percentage of lactate reached 1.6%. On this basis, in order to accumulate lactate, we reduced the activity of respiratory chain by deleting the ubiX gene, which is involved in the synthesis of coenzyme Q8. Moreover, we removed the dld gene to avoid the conversion of lactate to pyruvate during the fermentation. With these manipulations, the molar percentage of lactate in the polymer was improved to 14.1%, with an 81.7% (W/W) of polymer content. The test results indicated that the strategy of reducing the activity of respiratory chain effectively increased the lactate units in the polymer, and it contributed a new approach to change the content of monomer components in the polymer.

Interference research of umbilical cord mesenchymal stem cells on the pulmonary fibrosis in silicosis rats / 中华劳动卫生职业病杂志

Objective@#To explore the effect of the umbilical cord mesenchymal stem cells(UC-MSCs) on the pulmonary fibrosis in silicosis rats.@*Methods@#SPF male Sprague Dawley rats were randomly divided into control group, silica model group and UC-MSCs treatment group with 12 rats each group. SiO2 intra-tracheal injection(0.5 ml of 50 mg/ml/rat) were applied to silica model group and UC-MSCs treatment groups. After that UC-MSCs treatment group received 1 ml UC-MSCs suspension (3×106 cells/ml) by tail vein injection on the 29th, 36th, 43th and 50th day after exposure to the first silica suspension. On the 60th and 75th day after exposure to silica suspension, all animals were examed for pulmonary CT. Then the rats were euthanized on 75th day after the first exposure to silica.Lung's histopathological examination of the rats from all the groups were carried out. The content of hydroxyproline in lungs, TGF-β1 and IL-6 in serum were examined.@*Results@#The lung's histopathological examination showed no obvious inflammatory cell and no fibrosis in the lung tissue of the control group, there were a lot of inflammatory cell aggregation and collagen fiber deposition in silica model group, while in the UC-MSCs intervention group and treatment group, there were less inflammatory cells and collagen fiber. The rats from silica model groups had higher HYP, TGF-β1 and IL-6 than the rats from UC-MSCs treatment group and control group. Lung fields of rats in the control group were clear and no obvious high-density shadow. Different-sized granular high-density shadows or reticular fibrous shadows were found diffusely distributed in the lungs of the rats in silica model group. Lung field of rats in UC-MSCs intervention group and treatment group were less high density shadows, and more clear.@*Conclusion@#UC-MSCs can alleviate the pulmonary fibrosis in silica model rats through regulating the secretion of some fibrosis related cytokines.

Reform thought and practice on training application-oriented talents of medical laboratory technology specialty by the trinity of "teaching, clinics and research" method / 中华医学教育探索杂志

Medical Laboratory Technology now belongs to the first-level discipline of medical technology and focuses on the cultivation of technical talents.At present,there are some problems in this professional education,such as emphasizing theoretical knowledge teaching,neglecting practical skills training,weak practicability of some courses and graduates' lack of professional quality.In this study,we have combined "Teaching,Clinical and Scientific research" together.By improving the teaching staff,increasing the proportion of experimental courses,clinical skills and scientific research training,relying on "verification-synthesis-design" experiments,laboratory opening and undergraduate scientific research competitions,we have carried out professional personnel training,and have achieved better educational results,which is also helpful to the development of laboratory discipline.

Effect of bone marrow mesenchymal stem cell transplantation on silicosis fibrosis in rats / 重庆医学

Objective To study the effects of bone mesenchymal stem cells(BMSCs)transplantation on pulmonary tissue fibrosis in silicosis rats.Methods Forty-two healthy female SD rats were randomly divided into 4 groups:group A,B,C and D.The group A,B and C received intratracheal injection of silica crystals(SiO2)to establish silicosis mouse models,while the group D received the infusion of physiological saline.BMSCs were injected by the tail vein at 6 h after modeling or on 42 d in the group B and C.Six rats in each of group A,B,and D were sacrificed on 14 d,and other 6 rats in each group were sacrificed on 56 day.The morphological changes of the lung tissues were observed by HE staining;the content of hydroxyproline(Hyp)in the lung tissue homogenate was detected by ELISA.Results The lung tissue pathological changes in the group A were dominated by acute inflammatory response on 14 d after model construction,and fibrosis reaction on 56 d;the pathological changes in the group B and C were lighter than those in the group A;while no obvious abnormality was found in the group D.The Hyp level of pulmonary tissue in the group A,B and C was significantly increased compared with the group D(P=0.000);which on 14 d after BMSCs treatment in the group B and C was significantly decreased compared with the group A(P=0.000,P=0.035),and which on 56 d after treatment in the group B was still significantly decreased(P=0.016).Conclusion No matter in the pathological stages of pulmonary alveolitis and fibrosis,BMSCs transplantation may postpone the pathological process of pulmonary fibrosis in silicosis rats.

Analysis of clinical and pathological characteristics of high-risk HPV-negative carcinoma of the uterine cervix / 中华妇产科杂志

Objective To study the clinical and pathological characteristics of HPV-negative cervical cancer patients. Methods Retrospective analysis of 785 cervical cancer patients in Guangdong Women and Children Hospital from Jan. 2005 to Oct. 2015. By detecting high-risk HPV infection by flow-through hybridization genechip technique. Results (1) Among 785 cases of cervical cancer, 71 cases were negative for HPV infection tested by genechip technique, accounting for 9.0%(71/785), and the relative light units/cut off (RLU/CO) ratios of these 71 cases were less than 1 by hybird captureⅡ(HC-Ⅱ) methods. The results showed that the positive coincident rate of genechip technique detecting result with HC-Ⅱmethod was 100.0%(71/71). (2) There was no difference between 43 (60.6%) cases from 41-55 years old of 71 cases of HPV-negative patients and 392 (54.9%) cases from 41-55 years old of 714 cases of HPV infection patients (χ2=15.63, P=0.571). Among 71 cases of HPV-negative patients, 32 cases of patients with doing TCT, 6 (18.8%) cases for normal, 10 (31.2%) cases for atypical squamous cells of undetermined significance (ASCUS), 3 (9.4%) cases for atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H), 3(9.4%) cases for low-grade squamous intraepithelial lesions (LSIL), 8 (25.0%) cases for hight-grade squamous intraepithelial lesions (HSIL), 2 (6.2%) cases for squamous cell carcinoma (SCC). And there were 391 cases of patients with doing thin-prep cytologic test (TCT) of 714 HPV infection patients, 60 (15.3%) cases for normal, 61 (15.6%) cases for ASCUS, 28 (7.2%) cases for ASC-H, 29 (7.4%) cases for LSIL, 164 (41.9%) cases for HSIL, 49 (12.5%) cases for SCC. There was no difference of TCT between HPV infection and HPV-negative patients (P>0.05). Among 70 cases from 71 patients with negative for HPV infection, there were 8(11.4%) cases in stage Ⅰa, 26 (37.1%) cases in stage Ⅰb1, 12 (17.1%) cases in stageⅠb2, 11 (15.7%) cases in stageⅡa, 10 (14.3%) cases in stageⅡb, 3 (4.3%) cases in stageⅢ-Ⅳ. There were 118(16.6%) cases in stageⅠa, 261 (36.8%) cases in stageⅠb1, 72 (10.1%) cases in stageⅠb2, 152 (21.4%) cases in stageⅡa, 87 (12.3%) cases in stageⅡb, 20(2.8%) cases in stageⅢ-Ⅳin 710 cases of HPV infection patients, in which there were no difference of clinical stage between HPV infection and HPV-negative patients (P>0.05). Among 69 cases from 71 patients HPV-negative infection, there were 51 (73.9%) cases for squamous carcinoma, 13 (18.8%) cases for adenocarcinoma, 5(7.2%) cases for adenosquamous carcinoma;and 593 (87.2%) cases for squamous carcinoma, 38 (5.6%) cases for adenocarcinoma, 39 (5.7%) cases for adenosquamous carcinoma, 10 (1.5%) case for others were in 680 patients of HPV infection, in which there was significant difference of adenocarcinoma between HPV infection and HPV-negative patients (χ2=11.96, P=0.001). Conclusions Flow-through hybridization genechip technique is the method of high sensitivity to detect high-risk type HPV, as like HC-Ⅱ method. HPV-negative of cervical cancer occurs mainly in 41-55 years old. Adenocarcinoma incidence is significantly higher in HPV-negative cases than those patients with infection of HPV positive.

Radiofrequency Ablation for the Treatment of Thyroid Benign Nodules:a Report of 61 Cases / 中国微创外科杂志

Objective To evaluate therapeutic effects of radiofrequency ablation for thyroid benign nodules . Methods By using the radiofrequency electrode (18G ×7 cm, working segment length =1 cm), a total of 61 patients with 87 thyroid benign nodules underwent ultrasound-guided radiofrequency ablation in our department from February 2013 to May 2014.The maximum diameter , volume, and volume-reduction ratio (VRR) were measured by ultrasound at 1, 3, 6, and 12 months after operation. Results The 61 patients with 87 thyroid benign nodules were successfully treated with radiofrequency ablation , including 3 cases receiving secondary ablation after 1 month.The maximum diameter of thyroid nodules was (2.24 ±0.63) cm preoperatively, and (1.82 ±0.81) cm, (1.52 ±0.72) cm, (1.03 ±0.43) cm, and (0.56 ±0.36) cm at 1, 3, 6, and 12 months after ablation, respectively.The maximun volume was (5.37 ±1.85) ml preoperatively, and (4.41 ±2.16) ml, (3.19 ±1.92) ml, (1.34 ±0.61) ml, and (0.69 ±0.41) ml at 1, 3, 6, and 12 months after ablation, respectively.The VRR were (20.7 ±13.2)%, (48.6 ±16.1)%, (77.3 ±4.7)%and (88.7 ±5.6)%, respectively at 1,3,6, and 12 months after ablation.At the final follow-up, 51 (58.6%) nodules disappeared completely. Conclusion Ultrasound guided radiofrequency ablation for thyroid benign nodules is safe and effective .

Effects of indium on micronucleus formation in human peripheral blood lymphocytes / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the cytotoxicity of indium chloride (InCl₃) and its effects on micro-nucleus formation in primary human lymphocytes cultured in vitro.</p><p><b>METHODS</b>The CCK-8 assay was used to evaluate the cytotoxicity of 24 h exposure to different concentrations of InCl₃(4, 40, 80, 200, 500, and 1 000 µmol/L) in lymphocytes cultured in vitro. The cytokinesis-block method was used to determine the micronucleus level in lymphocytes exposed to different concentrations of InCl₃and the effects of anti-oxidant vitamin C on micronucleus frequency.</p><p><b>RESULTS</b>Lymphocytes exposed to InCl₃of no less than 500 µmol/L had significantly lower survival rates than those in the control group (P < 0.05). Lymphocytes exposed to 80 µmol/L InCl₃had a significantly higher micronucleus frequency than those in the control group (P < 0.05). However, there was no further increase in micronucleus frequency of lymphocytes exposed to 200 µmol/L InCl₃. Lymphocytes cultured in whole blood and exposed to 500 or 1000 µmol/L InCl₃had a significantly increased micronucleus frequency than those in the control group (P < 0.001). The increase in micronucleus frequency of lymphocytes induced by indium could be partially antagonized by 20 or 100 µmol/L vitamin C.</p><p><b>CONCLUSION</b>InCl₃can induce an increase in micronucleus frequency of primary human lymphocytes cultured in vitro, which might be associated with DNA damage induced by oxidative stress.</p>

Effects of indium exposure on relative content of mitochondrial ND1 gene in human peripheral blood lymphocytes in vitro / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effects of indium exposure on the relative content of mitochondrial ND1 gene in lymphocytes.</p><p><b>METHODS</b>Venous blood was obtained from 14 healthy workers and anticoagulated with heparin. Blood lymphocytes were separated and divided into three tube cultures. For two tubes in the exposed group, indium chloride was added to final concentrations of 0.2 mmol/L and 0.8 mmol/L, respectively. For one tube in the control group, an equal volume of normal saline solution was added. After incubation for 72 h, the relative content of mitochondrial gene in each group was determined using quantitative real-time PCR.</p><p><b>RESULTS</b>Lymphocytes exposed to 0.8 mmol/L indium chloride had a significantly higher relative content of mitochondrial gene than those exposed to 0.2 mmol/L indium chloride and those in the control group (P < 0.05, P < 0.05).</p><p><b>CONCLUSION</b>Lymphocytes exposed to a high concentration of indium and its compounds have an elevated relative content of mitochondrial ND1 gene, indicating increased oxidative DNA damage induced by exposure to a high concentration of indium and its compounds.</p>

Risk analysis and assessment of occupational hazard fromindiumand its compounds in manufacture of liquid crystal display panel / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To understand the exposed positions and levels of indium and its compounds in manufacture of liquid crystal displays, and to evaluate the degree of occupational hazard from indium and its compounds.</p><p><b>METHODS</b>On-site investigation of occupational health, occupational hazard monitoring, and occupational health examination were used to evaluate the degree of occupational hazard from indium and its compounds in three manufacturers of liquid crystal display panel in Shenzhen, Guangdong, China.</p><p><b>RESULTS</b>The time-weighted average (TWA) and short-term exposure limit (STEL) concentrations of indium and its compounds to which sputtering machine operating positions were exposed were less than 0.002~0.004 mg/m³ and 0.006~0.007 mg/m³, respectively, both of which complied with the National Hygienic Standard (PC-TWA = 0.1 mg/m³; PC-STEL = 0.3 mg/m³); the TWA and STEL concentrations of indium and its compounds to which grinding positions were exposed were 0.114~2.98 mg/m³ and 0.31~10.02 mg/m³, respectively, both of which exceeded the National Hygienic Standard with the highest concentration 33-fold higher than the standard. No significant health damages were found in exposed workers according to the results of occupational health examination.</p><p><b>CONCLUSION</b>The grinding positions are the key to the control of occupational hazard from indium and its compounds in manufacture of liquid crystal display panel. The workers should be equipped with the anti-particulate full-face respirator, which is an effective way to prevent occupational hazard from indium and its compounds.</p>

Determination of indium in whole blood by graphite furnace atomic absorption spectrometry / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate the sensitization effect of different chemical modifiers in the determination of indium in whole blood by graphite furnace atomic absorption spectrometry, and to develop a new method for the determination of indium in whole blood.</p><p><b>METHODS</b>A mixture of 0.3% HNO3 (V/V) + 0.1% Triton X-100 (V/V) was used as a diluent, and a solution of 1 000 µg/ml Pd (NO3)2 + 3 000 µg/ml Mg (NO3)2 was used as modifier. After being diluted five times, the concentration of indium of the blood was directly determined by graphite furnace atomic absorption spectrometry.</p><p><b>RESULTS</b>The detection limit of the method was 0.33 µg/L, the linear range was 0.33~100.00 µg/L, the relative standard deviation was 1.43%~2.65%, and the recovery rate was 98.3%~105.3%.</p><p><b>CONCLUSION</b>The method is simple and fast and has high recovery and precision, and it is suitable for the determination of indium in whole blood.</p>

Serum levels of macrophage migration inhibitory factor in patients with vitiligo vulgaris / 中华全科医师杂志

Serum levels of macrophage migration inhibitory factor (MIF) were measured with enzyme linked immunosorbent assay (ELISA) in 60 patients with vitiligo vulgaris (case group) and 30 healthy individuals (control group).The case group was further divided into two subgroups:progressive stage (n =30) and stable stage (n =30).The association of MIF level with vitiligo area and severity index (VASI) score of disease was analyzed.The results showed that the serum MIF levels in patients with progressive stage were significantly higher than those in stable stage and in healthy controls (79.8 ± 38.0) μg/L vs.(48.4 ± 17.6) μg/L vs.(29.6 ± 22.1) μg/L,respectively,both P ＜ 0.001.Serum MIF levels were positively correlated with VASI scores of patients (r =0.48,P ＜0.001).The results indicate that MIF may be involved in the pathogenesis of vitiligo vulgaris.

Clinical Study onDu-Huo Ji-ShengDecoction with Low Frequency Ultrasonics in Treatment of Knee Osteoarthritis / 世界科学技术-中医药现代化

This study was aimed to observe the clinical effect of Du-Huo Ji-Sheng (DHJS) decoction with low frequency ultrasonics in treatment of knee osteoarthritis (KOA) by evaluating the visual analogue scale (VAS), Lysholm score and the expression levels of transforming growth factor-β (TGF-β) and matrix metalloproteinase-3 (MMP-3). A total of 60 KOA outpatients were randomly divided into the treatment group and the control group, with 30 cases in each group. Patients in the treatment group were treated with DHJS decoction and low frequency ultrasonics therapy, while patients in the control group were treated with ibuprofen and low frequency ultrasonics. The treatment was given once a day for two weeks. The pretreatment and posttreatment VAS, Lysholm score and expression levels of TGF-β and MMP-3, which were detected by ELISA, were recorded. The results showed that compared with the control group, DHJS decoction with low frequency ultrasonics therapy could alleviate pain as well as improve the activity of joint function among KOA patients. It could also increase the content of TGF-βin serum, and suppress the level of MMP-3. It was concluded that low frequency ultrasonics could enhance the transdermal absorption of DHJS decoction, relieve the clinical symptoms of KOA patients.

Diagnostic value of Matrix metalloproteinase-9 and Epidermal growth factor in uterine myoma / 中国综合临床

Objective To discuss the diagnostic value of Matrix metalloproteinase-9 (MMP-9) and Epidermal growth factor (EGF) in uterine myoma.Methods Seventy-three patients with uterine leiomyoma treated by surgery as treatment group,and 65 healthy women were selected as control group.The levels of MMP9 and EGF in both groups were detected by enzyme-linked immunosorbent assay method.Results The levels of MMP-9and EGF in treatment group were significantly higher than those of control group((1.72±0.36) mg/L vs.(1.28±0.25) mg/L, (342.16± 128.65) ng/L vs.(209.84± 100.34) ng/L;t =8.241,6.678;P ＜0.01).There were no relationships between the levels of MMP-9 and EGF with maximum diameter of uterine myoma (P ＞0.05).Conclusion Serum levels of MMP-9 and EGF have some clinical value in diagnosing uterine leiomyoma.

Expressions of miRNA27a and miRNA451 in ovarian cancer and breast cancer cells and its correlation with drug resistance by targeting MDR1/P-glycoprotein / 中国癌症杂志

Background and purpose: Resistance of cancer cells to chemotherapy is a major clinical obstacle to successful treatment and leads to poor prognosis for the patients. MicroRNA (miRNA) plays a vital role in tumor cells response to chemotherapeutic agents. This study plans to investigate the expressions of miRNA27a and miRNA451 in ovarian cancer and breast cancer cells and its correlation with drug resistance. Methods:A2780/Taxol cells were established using stepwise selection;Stem-loop quantitative real-time PCR (stem-loop RT-PCR) was used to detect expression of miRNA27a and miRNA451 in ovarian cancer and breast cancer cells. The A2780 and A2780/Taxol cells were transfected with the mimics or inhibitors of miRNA27a or negative control (NC) RNA and the mimics of miRNA451 or NC were transfected into MCF-7/ADM cells by LipofectamineTM 2000. The expression levels of MDR1 mRNA and P-glycoprotein (P-gp) were examined using RT-PCR and Western blot respectively. MTT method was used to analyze drug sensitivity. Results:The expression of miRNA27a was an average of (2.2±0.30) times higher in A2780/Taxol cells than in A2780 cells, with a significant difference between the two groups (P<0.05). The expression of miRNA451 was lower by 84%in MCF-7/ADM cells than in MCF-7 cells, with a signiifcant difference between the two groups (P<0.05). A2780/Taxol cells transfection with inhibitors of miRNA27a showed that the levels of MDR1 mRNA was decreased by (39±0.14)%, P-gp level [(26±5.3)%] decreased compared with the NC group [(43±6.7)%], the IC50 (0.53μmol/L) was less than the NC group (6.8μmol/L), and there was a signiifcant difference between two groups (P<0.05). Transfection of A2780 cells with mimics of miRNA27a led to increase MDR1 mRNA expression by (121±0.11)%and decrease the sensitivity to paclitaxel (IC50:0.2μmol/L vs 0.06μmol/L). There was a signiifcant difference between two groups (P<0.05). Transfection of MCF-7/ADM cells with mimics of miRNA451 showed that expression of MDR1 mRNA was decreased by (65±12)%, P-gp [(31±19)%] was less than the NC group [(83±12)%], the sensitivity of cells to adriamycin enhanced and the IC50 of adriamycin (4.61μmol/L) was less than the NC group (26μmol/L), and there was a signiifcant difference between two groups (P<0.05). Conclusion:The expressions of miRNA27a and miRNA451 are deregulated in A2780/Taxol and MCF-7/ADM cells respectively, which may play vital roles in drug resistance by regulating MDR1/P-gp expression directly or indirectly.

Establishment and evaluation of method to collect rat gingival crevicular fluid by using absorbent paper points / 吉林大学学报(医学版)

Objective To establish and evaluate the method to collect the rat gingival crevicular fluid (GCF)by using absorbent paper points, and to lay foundation for analysis on GCF.Methods 20 healthy male rats were selected and randomly divided into GCF group and saliva group.The GCF of the right upper molar gingival trough of the rats in GCF group and the saliva of the rats in saliva group were collected by using 1 5# absorbent paper points.The SDS-PAGE analysis and abundance detection were applied to analyze the protein bands of the samples in two groups.Results The SDS-PAGE analysis identified the proteins at 77 000,66 000,55 000,51 000,and 28 000,especially 66 000 in GCF group.While saliva group had lower brightness protein bands at 66 000,60 000, and 48 000.The data of protein abundance of 66 000 between two groups had statistically significant difference (P<0.05).Conclusion The number and types of the protein bands are different between GCF Group and saliva group,so using 15# absorbent paper points can collect the rat GCF successfully.