ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of cyclopamine on metastatic ability of human esophageal cancer EC109 cells and explore the possible mechanism.</p><p><b>METHODS</b>Transwell chamber assay and angiogenesis assay were used to examine the metastatic ability, invasiveness and angiogenesis of EC109 cells treated with cyclopamine for 48 h. The expression of Gli-1 mRNA was detected using RT-PCR, and Western blotting was used to examine the protein expressions of Gli-1, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF).</p><p><b>RESULTS</b>Inhibition of the hedgehog signaling pathway by cyclopamine suppressed the migration, invasion, and angiogenesis of EC109 cells. Cyclopamine treatment significantly lowered the expression of Gli-1 mRNA (P<0.05) and the protein expressions of Gli-1, MMP-9 and VEGF (P<0.05).</p><p><b>CONCLUSION</b>Cyclopamine can significantly inhibit the metastatic capacity of EC109 cells possibly by down-regulating MMP-9 and VEGF expression as a result of Gli-1 inhibition.</p>
Subject(s)
Humans , Cell Line, Tumor , Esophageal Neoplasms , Metabolism , Pathology , Gene Expression Regulation, Neoplastic , Matrix Metalloproteinase 9 , Metabolism , Neoplasm Metastasis , RNA, Messenger , Genetics , Signal Transduction , Transcription Factors , Metabolism , Vascular Endothelial Growth Factor A , Metabolism , Veratrum Alkaloids , Pharmacology , Zinc Finger Protein GLI1ABSTRACT
0.05). Conclusion: Elderly patients with stable or surgically treated cardiac disease should not be denied pulmonary resection for primary lung cancer. Cardiological assessment should be sought for all patients with known or suspected cardiac disease prior to pulmonary surgery. In our experience we advocate myocardial revascularisation prior to lung resection in patients with severe left main stem or triple-vessel coronary artery disease along with careful re-evaluation of those patients who underwent coronary artery surgery greater than 5 years previously.
ABSTRACT
Objective:To explore the perioperative treatment for esophageal cancer patients concomitent with diabetes.Methods:74 operation cases of esophageal and gastric cardia cancer associated with diabetes were analyzed retrospectively from 1976 to July 2003.Results:The occurrence of esophageal and gastric cardia cancer patients concomitent with diabetes was 6.1% among the esophageal cancer operative cases in the same period. The morbidity was 12.2% (including pulmonary infections in 2 cases,incision infection in 1,anastomosis fistula in 2,HHNC in 2 and hypoglycemia in 2 cases).The perioperative mortality was 1.4% with one patient dying of respiratory failure caused by pulmonary infection.Conclusion:Esophageal and gastric cardia cancer patients associated with diabetes are found to be common in thoracic operations (6.1%).It is crucial to control these patients for low morbidity and mortality through enough preparation,satisfactory aneathesial management and good nutrition support perioperatively.