Comparative Study on the Level of Resistin,Adiponectin between Blood Stasis Syndrome and Non-Blood Stasis Syndrome in Diabetic and Primary Hypertension Patients / 中国中医药信息杂志

Objective To investigate the correlation between resistin,adiponectin and the blood stasis syndrome in diabetic and primary hypertension patients.Method The primary hypertension and diabetes patients were drawn vein blood to make plasma and serum.Enzyme linked immunosorbent assay(ELISA) method was used in checking serum adiponectin and resistin.Radioimmuoassay method was applied in checking serum insulin,leptin,ET and tumor necrosis factor(TNF).The fasting blood sugar,total cholesterol(TC),triamid-glycerin(TG),high dense lipoprotein-cholesterol(HDL) and low dense lipoprotein-cholesterol(LDL) were detected by the chemi-enzymic method.The relation between the indexes and blood stasis syndrome was analyzed by SAS9.1 for windows.Results The detectable rate of the blood stasis syndrome in the diabetic patients was 0.423,while 0.246 in the primary hypertension patients.The difference was significant in statistics.Incidence of blood stasis syndrome increased with the course of illness.Compared with the non-blood stasis syndrome group,the content of leptin,ET and TNF in the blood stasis syndrome group significantly raised,but the content of adiponectin and insulin diminished.The content of resistin did not have obviously change.Compared with the primary hypertension patients,the level of leptin,adiponectin,resistin,insulin in the diabetic patients raised,but there wasn't significant difference in the level of ET and TNF.Conclusions There is negative correlation between adiponectin and the blood stasis syndrome.The link between obesity and the blood stasis syndrome may be through fat factor regulating vasoactive factor.The blood stasis syndrome caused by obesity maybe have nothing to do with the consistency of resistin.

Effect of Ningxian Granule on Brain-derived Neurotrophic Factor Protein Expression in Rats with Pentylenetetrazol- Induced Epilepsy / 广州中医药大学学报

[ Objective ] To investigate the mechanism of Ningxian Granule (NG), a compound prescription with lucidity-ascending and turbidity-descending actions. [ Methods ] Forty SD rats were randomized into 5 groups: A (normal), B (model), C (low-dose NG, 5 g/kg), D (high-dose NG, 10 g/kg) and E (phenobarbital, 60 mg/ kg). Except Group A, the others were epileptic rats induced by pentylennetetrazol (PTZ, 35 mg/kg). NG and phenobarbiatal were given to different groups accordingly, once a day for 4 weeks. The effects of NG on brain-derived neurotrophic factor (BDNF) protein expression was observed by using immunohistochemistry technique. [Results] The number of positive neuron increased both in the hippocampus and cerebral cortex of epileptic rat brain (P