ABSTRACT
ObjectiveTo investigate the relationship between Dicer expression and clinicopathological characteristics and prognosis by detecting the expression of Dicer in hilar cholangiocarcinoma tissues and cells.MethodsThe expression of Dicer in tissues was detected using immunohistochemistry.Western blotting and RT-PCR were used to investigate Dicer expression in QBC939 and HIBEpic cells.The relationship between Dicer expression and clinicopathological characteristics was analyzed.A Kaplan-Maier analysis was performed to analyze the disease-free survival (DFS) and overall survival (OS) after radical surgical resection of hilar cholangiocarcinoma.ResultsWhen compared to control,Dicer was significantly down-regulated in hilar cholangiocarcinoma tissues (P＜0.05) and in QBC939 (P＜0.05).The expression of Dicer was higher in well differentiated adenocarcinoma than poorly and moderately differentiated tumours. Univariate analysis showed low expression of Dicer protein was significantly correlated with short disease-free survival and overall survival of patients with hilar cholangiocarcinoma after radical surgical resection (P＜0.01). Multivariate analysis revealed that the expression of Dicer was the most important factor for predicting prognosis after radical surgical resection of hilar cholangiocarcinoma (P＜0.05).ConclusionsDicer could be used as a prognostic marker for hilar cholangiocarcinoma.
ABSTRACT
Objective To summarize our experience in the management of calculous atrophic cholecystitis by laparoscopic choleycystectomy(LC). Methods 56 cases of calculous atrophic cholecystitis treated by LC from 1997 to 2000 were analyzed retrospectively. Results 52 cases treated by LC including antergrade resection in 39 ones, retrograde resection in 13 ones were successfully completed. 4 patients were converted to open cholecystectomy, 3 of whom underwent choledochotomy and T-tube drainage. There was abdominal cavity drainage in 8 cases. 56 patients were an cured. Bile leakage occurred in 2 cases, and the patients were cured by conservative treatment. Conclusions It's key point to perform LC successfully for patients with calculous atrophic cholecystitis by getting well known of the junction site of ampulla and cystic duct, selecting indications of LC strictly, converting to open operation as necessary and putting preventive drainage- tube.