ABSTRACT
Purpose:To evaluate multiple biomarkers of colorectal tumor and their potential usage in automated diagnosis of colorectal cancer.Methods:10 normal colonic mucosae, 30 colorectal adenomas and 53 colorectal carcinomas with complete follow-up data were examined for multiple biomarkers(DNA contents, AgNOR, PCNA and nuclear morphometry) with quantitative computed processed imaging analysis.Results:The values of most of the parameters analyzed increased in accordance with the normal mucosa-adenoma-adenocarcinoma sequence, indicating a marked tendency for progression of colorectal malignancy. Among those parameters, DNA index,positive rates, counts, density and aspect factor of AgNOR, positive rates and density of PCNA, shape factor, width and density of nucleus were demonstrated to be relatively valuable indices.Conclusions:The quantitative evaluation of some indices of colorectal tumor can provide reproducible data for differential diagnostic of this tumor.