ABSTRACT
Objective To study the browning-related genes of Poria cocos mycelia,so as to provide a basis for their further verification and to discuss the browning molecular mechanism of Poria cocos.Methods Four samples of normal and brown Poria cocos mycelia were analyzed by transcriptome sequencing.After aligned with the reference genome sequence of Poria cocos,the expression levels of the genes in different samples were analyzed.Results A total of 12 383 transcripts were identified.Among them,1 017 genes were firstly found,and 260 genes were functionally annotated.Based on the alignment results,336 common differentially-expressed genes overlapped between the normal mycelia and 3 browned mycelia were obtained,and part of them were evaluated.Conclusion Transcriptome sequencing results showed that plenty of differentially-expressed genes were found in brown Poria cocos mycelia,and the difference of the expression levels of some genes was up to several hundred folds or even thousands of folds,whose related-functions were worth of further analysis.
ABSTRACT
Objective To investigate the role of miR-126 (micro RNA-126) in rat endothelial progenitor cells (EPCs) proliferation and migration and the starget gene of miR-126 by bioinformatics and experimental survey.Method EPCs were transfected with control oligoes and miR-126 mimics or inhibitor by electroporation.MTT was performed to evaluate the growth of EPCs subjecting to miR-126 overexpression.Cell migration analysis was done by wound healing and transwell assay.The target genes of miR-126 were predicted by TargetScan and validated by Western blot.Result (1) miR-126 mimics promoted EPCs growth at 24 h post cell transfection (P ＜ 0.01).In contrast,the EPCs growth was immue from miR-126 application at 48 and 72 h.(2) Both the wound healing and transwell assay show that miR-126 promotes EPCs migration (P ＜ 0.01) and miR-126 inhibitor inhibits EPCs migration (P ＜ 0.01).(3)It is predicted that KANK2 is the potential target gene of miR-126 by TargetScan online software.(4) The results of Western blot indicated that miR-126 mimics repress the expression of KANK2 compared with NC but miR-126 inhibitor enhances KANK2 expression.Conclusions miR-126 has a transient effect on the promotion of EPCc growth.miR-126 promotes EPCs migration and targets KANK2 protein.