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Objective To explore the value of 18F-fluorodeoxyglucose (FDG) PET/CT for therapeutic assessment in patients with bone metastases from differentiated thyroid carcinoma (DTC) after 131I treatment.Methods Between January 2006 and August 2017,35 DTC patients (11 males,24 females,median age 60 years) with bone metastases who were treated with 131I and underwent 18F-FDG PET/CT scan were retrospectively analyzed.Therapeutic response assessment was based on morphological changes or the maximum standardized uptake value (SUVmax) changes of bone metastases,and the agreement (Cohen's Kappa coefficient) between two methods was analyzed.The progression-free survival (PFS) was estimated by Kaplan-Meier survival analysis and compared among patients with different treatment response (log-rank test).Results Morphological changes were consistent with SUVmax changes in 82.1％(23/28) of patients with positive 18F-FDG uptake (Kappa=0.731,95％ CI:0.628-0.834,P＜0.01).Five patients had stable disease according to morphological assessment,while 18F-FDG PET indicated they had metabolic response or disease progression.The serum thyroglobulin (Tg) levels confirmed the accuracy of 18F-FDG PET in 3 of those 5 patients.Compared with the patients with metabolically or morphologically progressive disease,patients who showed metabolically or morphologically stable disease or complete/partial response had significantly favorable prognosis (x2 values:4.132-6.543,all P＜0.05).Conclusions The therapeutic response based on metabolic criteria is in agree with that based on morphological criteria in most of the DTC patients with bone metastases.The SUV may act as a sensitive and efficient indicator of early therapeutic response or disease progression of bone metastases in DTC patients with positive 18F-FDG uptake.
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Objective To study the clinical value of 18F-FDG PET/CT in childhood neuroblastoma (NB). Methods 18F-FDG PET/CT was performed in 31 children diagnosed with NB. According to the treatment conditions, patients were divided into pre-therapy group, radiation and chemotherapy group, postoperative group, respectively. The positive rate, sites, and im-age characteristics of the primary lesion and metastasis lesion were analyzed through qualitative and quantitative analysis of the image. Results Twenty-one (67.7%) and fourteen (45.2%) patients were found positive in primary sites by CT and PET respectively. All cases (9/9, 100%) in pre-therapy group were found positive in primary lesions by PET, 3 positive cases (75.0%) in radiation and chemotherapy group and 2 positive cases (11.1%) in postoperative group. Twenty-one patients showed metas-tases (67.7%). Lymph nodes (16 cases) and bone (bone marrow) (13 cases) were the most common sites of metastasis followed by pleura, meninges, liver and retrobulbar inifltration, all of which showed increased FDG uptake. Two patients were found lesions in lungs by CT, but had no FDG uptake. SUVmax of primary lesions was signiifcantly different among pre-therapy, chemotherapy and postoperative group (H=13.89, P=0.001), and pre-therapy group had the highest value. Metastases (lymph nodes, bone and bone marrow, pleura, liver and meninges) in pre-therapy group had high SUVmax. Conclusions NB primary tumors are characterized by the increased FDG metabolism. PET can fully detect the distribution of NB metastases in whole body. Except the pulmonary metastasis, metastases in other positions show increased FDG uptake. PET has potential role in evaluating the efifcacy of radiation and chemotherapy, and identifying postoperative residual or recurrence.
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Chitosan is a natural biopolymer and is made up of D-glucosamine subunits linked by beta-(1,4) glycosidic bond. In recent years, the application of chitosan has attracted more and more attention because of its good biological function in cell biology. The properties of chitosan-based biomaterial are attributed to the physical properties and chemical composition of chitosan. The author of this paper summarized recent related studies and progresses of the influence of physical properties of chitosan on cell activity and cell mechanics property at home and abroad. The findings show that most studies mainly focused on the influence of chitosan and cell activity, while few were on cell mechanics property. The related studies of the influence of chitosan on cell will contribute to the explanation for the mechanism of the interaction between chitosan and cell, and provide the theoretical support for the further study.
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Animals , Humans , Cell Adhesion , Cell Differentiation , Cell Movement , Cell Proliferation , Chemical Phenomena , Chitosan , Chemistry , Pharmacology , Tissue Engineering , Tissue Scaffolds , ChemistryABSTRACT
Selectin/ligand interaction plays an important role in such biological processes as inflammatory reaction, tumor metastasis, etc. External forces affect dissociation of receptor-ligand bonds. A novel approach, upon optical trap technique, was developed in this study to investigate the dissociation of P-selectin/PSGL-1 (P-Selectin Glycoprotein Ligand 1) bindings. Stiffness of optical trap was calibrated with laser power using a viscous drag method. While P-selectin and PSGL-1 molecules were functionally coated on surfaces of glass beads, respectively, the dissociation of interacting molecule bond was studied by measuring the rupture force distribution. It was found that most probable rupture force increased with loading rate at < 25 pN/s. These results complemented and validated the current theory at low loading rates.
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Humans , Membrane Glycoproteins , Chemistry , Optics and Photonics , Protein Binding , Protein Structure, TertiaryABSTRACT
OBJECTIVE: To study the mechanism of rabbit lung injury caused by explosive decompression. METHODS: A total of 42 rabbits and 10 rats were served as the experimental animals. A slow recompression-decompression test and an explosive decompression test were applied to the animals, respectively. And the effects of the given tests on the animals were discussed. RESULTS: The slow recompression-decompression did not cause an obvious lung injury, but the explosive decompression did cause lung injuries in different degrees. The greater the decompression range was, the shorter the decompression duration was, and the heavier the lung injuries were. CONCLUSIONS: Explosive decompression can cause a similar lung injury as shock wave does. The primary mechanical causes of the lung injury might be a tensile strain or stress in the alveolar wall and the pulmonary surface's impacts on the inside wall of the chest.