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Objective:To analyze the influencing factors related to false-negative results of interferon-γ release assay (IGRA) QFT-GIT in patients with confirmed pulmonary tuberculosis.Methods:Clinical data of 389 patients with bacteriologically confirmed pulmonary tuberculosis who underwent QFT-GIT in Quzhou Hospital Affiliated to Wenzhou Medical University between January 1 and December 31 2020 were retrospectively analyzed. Univariate and multivariate logistic regression were used to analyze the influencing factors related to the false-negative results of QFT-GIT.Results:Among 389 confirmed patients, 347 cases had positive QFT-GIT results and 42 cases had negative results. Univariate analysis showed that the false-negative results of QFT-GIT were associated with low BMI, reduced CD4 + T lymphocyte count, decreased lymphocyte count, increased C-reactive protein, negative sputum smear, anemia, diabetes mellitus, malignant tumor and sepsis ( P<0.05 or P<0.01). Multivariate conditional logistic regression analysis showed that BMI <18.5 kg/m 2( OR=1.585, 95% CI 1.076-2.336), complicated with diabetes( OR=5.157, 95% CI 2.340-11.365), malignant tumors ( OR=5.596, 95% CI 2.048-15.295)and sepsis ( OR=4.141, 95% CI 1.042-16.459) were independent risk factors for the false-negative results of QFT-GIT ( P<0.05 or P<0.01). Conclusion:When the pulmonary tuberculosis patients are extreme emaciation, complicated with diabetes, malignant tumor or sepsis, the QFT-GIT results will be false negative.
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Objective To investigate the effect of pegylated interferon combined thymopeptideenteric-coated tablets on CXCR3, CCR6 and serum IL-2 and soluble ligand in patients with chronic hepatitis.Methods 125 patients with chronic hepatitis B patients from June 2015 to June 2016 in our hospital were selected and divided into observation group 62 cases and control group 63 cases.The observation group was treated with pegylated interferon combined thymopeptideenteric-coated tablets, and the control group was treated with thymopeptideenteric-coated tablets, the expression of T cells in patients with CXCR3 and CCR6 was detected by flow cytometry, the serum levels of interleukin-2 and soluble interleukin-2 receptorwere measured by enzyme-linked immunosorbent assay, and compared the treatment of two groups of patients.Results After treatment, the levels of IL-2 and sIL-2R in the observation groupwere (16.4 ±5.6), (9.4 ±2.1), significantly lower than that in the control group, while CXCR3 and CCR6 were (5.8 ±0.9), (5.54 ±1.2), significantly higher than the control group, the difference was statistically significant (P<0.05), the levels of HBsAg, HBeAg and HBV-DNA in the observation group were (1.5 ±0.4), (1.6 ±0.9), (1.6 ±0.3), significantly lower than those in the control group, the difference was statistically significant (P<0.05), the levels of ALT, AST, TBIL, IBIL in the observation groupwere (34.5 ±8.7), (31.4 ±6.9), (14.6 ± 6.9), (9.4 ±2.9), significantly lower than those in the control group, the difference was statistically significant (P<0.05).Conclusion Interferon combined thymopeptideenteric-coated tablets therapy for chronic hepatitis B is helpful to improve the level of chemokine receptor and inhibit the degree of inflammatory reaction.It can help to improve liver function and inhibit viral replication in patients with hepatitis B.It is better than the treatment of chronic hepatitis with thymosin alone.
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Vitamin D provides an adequate amount of calcium and phosphorus for the normal development and mineralization of a healthy skeleton,and it has been recognized as being essential for bone health for almost 100 years.Furthermore,Vitamin D is evolved into a hormone that has a wide diversity of biologic effects.This paper starts from a clinical case,then explores the reference interval of vitamin D and overviews the nutritional status of vitamin D in different regions of China.The laboratory test and standardization of vitamin D,the clinical significance and some misunderstandings in its popularization and application are all presented.The purpose of this paper is to let the public pay more and more attention to it, besides,there needs more deep understanding and thinking.
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Objective:To investigate the clinical significance of detecting peripheral blood myeloperoxidase in OSAHS patients. Method:Sixty cases of OSAHS patients in our hospital was selected as OSAHS group, which was subdivided into light, medium and heavy groups according to the PSG, each with 20 cases, and 20 cases of healthy persons were selected as control group; ELISA method was used to detect the MPO level of peripheral blood. 20 cases of severe OSAHS patients detect MPO level again three months after the fist detection, and analyze the correlation between serum MPO level and indicators of sleep respiratory related events. Result:①The level of peripheral serum MPO level positively correlated with the severity of the patients' condition. The control group, light, medium and heavy groups' serum MPO levels were (7.93±2.44), (10.58±3.31), (18.91±8.37), (29.03±10.06)ng/mg, respectively (F=19.102, P<0.05). ②OSAHS patients' peripheral blood MPO concentration had no correlation with gender, age and BMI, but was positively correlated with AHI and negatively correlated with LSaO₂ (P<0.05). ③Twenty cases of severe MPO and AHI patients with OSAHS were decreased and LSaO₂ increased, the difference was statistically significant (P<0.05). Conclusion:The detection of serum MPO in patients with OSAHS has important significance in judging the severity of the disease, evaluating the treatment effect and predicting the risk of cardiovascular disease in patients with severe OSAHS.
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Objective:To explore the efficacy and safety of different surgical methods in papillary thyroid carcinoma (PTC) patients with negative cervical lymph nodes (cN0) and to provide theoretical basis for the selection of surgical procedures. Methods:A total of 225 PTC cN0 patients were selected and divided into two groups according to the operation method. Only 110 patients underwent total thy-roidectomy, whereas the 115 cases in the lobectomy group underwent ipsilateral lobectomy and isthmus resection. Al patients were treated with ipsilateral central lymph node dissection. We analyzed both groups of patients in terms of surgery, hospitalization, pathological characteristics, complications, follow-up, and recurrence. Results:(1) The length of incision, blood loss, operation time, and length of stay were not significantly different between the two groups (P>0.05). (2) In the total resection group, the number of multiple foci of the cancer cases was significantly more than that of the lobectomy patients (P0.05). Between the two groups, the number of central lymph node dissection and total lymph node metastasis, and the number of cases with central lymph node metastasis were not significantly different (P>0.05). Likewise, the incidence rates of mistakenly cut parathyroid, extremely low blood calcium levels, temporary larynx return nerve paralysis, and superior laryngeal nerve injury were not significantly different between the two groups (P>0.05). No permanent hypocalcemia or permanent recurrent laryngeal nerve injury occurred. (3) All patients were followed up for 2-4.5 years. No recurrent cases were reported in the total resection group. For the lobectomy group, 8 patients with adenocarcinoma had contralateral tumor metastasis and underwent contralateral lobe resection, whereas 4 patients suffered from ipsilateral neck lymph node metastasis at the side area and underwent functional lymph node clearance. Significant differences were noted in the metastasis and recurrence rates between the two groups of patients (P<0.05). No deaths were reported during the follow-up period. Conclusion:For PTC cN0 patients, total thyroidectomy with joint ipsilateral central lymph node clearance can reduce the residual tumor, metastasis, and risk of recurrence,compared with surgery limited to the affected lobe and isthmus. Postoperative complications were not significantly increased.
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ObjectiveTo investigate the prevalence of cholelithiasis in the employees of Kailuan Corporation and to provide a reference for effective preventive measures. MethodsA total of 101 510 in-service or retired employees of Kailuan Corporation were recruited. All of them completed checkups organized by Kailuan Corporation from June 2006 to October 2007. The cholelithiasis examination data of 1133 employees were lost and the data of 100 377 employees were finally included in the statistical analysis. Questionnaire survey, blood biochemical examination, and ultrasound examination of the gallbladder were carried out. According to the ultrasound examination results, the subjects were divided into cholelithiasis group and non-cholelithiasis group. Comparison of the means between the two groups was made by t-test and comparison of the incidence rates between the two groups was made by chi-square test. The risk factors for cholelithiasis were assessed using multiple logistic regression analysis. ResultsThere were 80 129 males and 20 248 females in the 100 377 subjects. Their ages ranged from 18 to 98 years (mean, 51.89±12.66 years). There were 98 109 subjects without cholelithiasis and 2268 subjects (2.3%) with cholelithiasis. The females had a significantly higher incidence of cholelithiasis compared with the males (2.5% vs 2.2%, χ2=4.60, P=0033). The incidence rate of cholelithiasis increased with age in both men and women. According to the result of logistic regression analysis, sex, age, body mass index, occupation, fasting plasma glucose, high-sensitivity C-reactive protein (hs-CRP), and cholecystitis were all risk factors for cholelithiasis and the odds ratios (95% confidence interval) of them were 0.765(0.687-0.853), 1.439(1.388-1493), 1.403(1.282-1.535), 0.821(0.706-0.955), 1.234(1.075-1.416), 1.080(1.022-1.142), and 12.519(10678-14678), respectively. ConclusionPeople with advanced age, female sex, obesity, hyperglycemia, high hs-CRP, cholecystitis, or unhealthy lifestyle have high risk of cholelithiasis.
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ObjectiveTo investigate the prevalence of cholelithiasis in the employees of Kailuan Corporation and to provide a reference for effective preventive measures. MethodsA total of 101 510 in-service or retired employees of Kailuan Corporation were recruited. All of them completed checkups organized by Kailuan Corporation from June 2006 to October 2007. The cholelithiasis examination data of 1133 employees were lost and the data of 100 377 employees were finally included in the statistical analysis. Questionnaire survey, blood biochemical examination, and ultrasound examination of the gallbladder were carried out. According to the ultrasound examination results, the subjects were divided into cholelithiasis group and non-cholelithiasis group. Comparison of the means between the two groups was made by t-test and comparison of the incidence rates between the two groups was made by chi-square test. The risk factors for cholelithiasis were assessed using multiple logistic regression analysis. ResultsThere were 80 129 males and 20 248 females in the 100 377 subjects. Their ages ranged from 18 to 98 years (mean, 51.89±12.66 years). There were 98 109 subjects without cholelithiasis and 2268 subjects (2.3%) with cholelithiasis. The females had a significantly higher incidence of cholelithiasis compared with the males (2.5% vs 2.2%, χ2=4.60, P=0033). The incidence rate of cholelithiasis increased with age in both men and women. According to the result of logistic regression analysis, sex, age, body mass index, occupation, fasting plasma glucose, high-sensitivity C-reactive protein (hs-CRP), and cholecystitis were all risk factors for cholelithiasis and the odds ratios (95% confidence interval) of them were 0.765(0.687-0.853), 1.439(1.388-1493), 1.403(1.282-1.535), 0.821(0.706-0.955), 1.234(1.075-1.416), 1.080(1.022-1.142), and 12.519(10678-14678), respectively. ConclusionPeople with advanced age, female sex, obesity, hyperglycemia, high hs-CRP, cholecystitis, or unhealthy lifestyle have high risk of cholelithiasis.
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Objective To explore the biological mechanism of the Lingering Toxins Theory in ulcerative colitis (UC) by clinical trials. Methods Forty patients with UC at mild, moderate active stage and at remission stage were enrolled into the experiment. Twenty cases of UC patients at active stage were divided into Chinese medicine intervention group and western medicine treatment group, 10 in each group. Twenty cases of UC patients at remission stage were also evenly divided into Chinese medicine intervention group and western medicine treatment group. All patients were given conventional western medicine treatment, and additionally, Chinese medicine intervention group were given oral use of Chinese medicine decoction, the treatment course covering 8 weeks. Before and after treatment, plasma matrix metalloproteinase-1 (MMP -1), tissue inhibitor ofmetalloproteinase-1 (TIMP-1) and prostaglandin E2 (PGE2) expression levels were observed by enzyme-linked immunosorbent assay (ELISA) . Results Plasma MMP-1,TIMP-1, and PGE2 expression levels in UC patients were positively correlated with disease severity (P0.05) . After treatment, the expression levels of MMP-1 and TIMP-1 in remission-stage patients of Chinese Medicine intervention group were decreased (P0.05) . Conclusion There is a relation between plasma MMP-1, TIMP-1 expression levels and the “lingering toxins” in the field of traditional Chinese medicine. The biological mechanism of toxins-removing therapy in treating UC may be associated with the decrease of the plasma MMP-1 and TIMP-1 expression levels.
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[Abstract ] Objective To understand the infection status of hepatitis B among population of health examination in Chongqing ,and to analyze the modes of hepatitis B outcomes .Methods The 30170 cases of serological results of hepatitis B (HB‐sAg① ,HBsAb② ,HBeAg③ ,HBeAb④ and HBcAb⑤ ) among the individuals of health examination in our hospital from June to December 2013 were performed the retrospective analysis .Results The positive rate of HBsAg among the population of health ex‐amination aged 0-96 years old in Chongqing was 8 .02% (2 419/30 170) ,and the differences in the positive rates among different age groups and between different genders had statistical significance (χ2 =188 .045 ,67 .813 ,P<0 .01) .The infection rate of HBV was 28 .64% (8 641/30 170) ,and the difference in the infection rates among different age groups and between different genders also had statistical significance(χ2 =202 .347 ,36 .171 ,P<0 .01) .30 170 cases of hepatitis B detection results of 5 indexes were conclu‐ded into 14 kinds of modes .As the two common modes of healthy population ,all negative and single HBsAb positive ,their percenta‐ges were 25 .76% (7 772/30 170)and 45 .60% (13 757/30 170)respectively .As the common modes of HBV infection ,“① ④ ⑤”posi‐tive ,“① ③ ⑤” positive and“① ⑤” positive ,their percentages were 6 .18% (1 863/30 170) ,0 .59% (177/30 170) and 1 .21% (366/30 170) respectively .Conclusion The positive rate of HBsAg and the infection rate of HBV among population of health examina‐tion in Chongqing are close to or lower than the national average levels .Analyzing the different combination modes can reflect the different situation of HBV infection and has the guidance significance for the diagnosis and prognosis of the disease .
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Objective To investigate the effects of flurbiprofen axetil for preemptive analgesia on hemodynamics and stress levels in patients undergoing neurosurgery.Methods 60 patients undergoing neturosurgery were enrolled in the study.The patients were randomly divided into the flurbiprofen group (Group F,n =30) or the control group (Group C,n =30).HR,MAP and serum norepinephrine (NE),epinephrine (E) levels were measured at 30min before the end of operation (T0),immediately after the end of operation (T1),immediately after extubation (T2).Results The changes in MAP,HR and serum NE,E levels in Group F at T1 and T2 were less than those of Group C (all P ＜ 0.0 1).Conclusion Flurbiprofen axetil for preemptive analgesia may suppress the stress response during recovery period in neurosurgical patients,and keep the hemodynamic index stable.
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BACKGROUND: The molecular weight of fibroin is inevitably reduced in the course of the dissolution of regenerated fibroin fiber, and this is why we have not entered the stage of the practical application of the regenerated fibroin fiber till now.OBJECTIVE: To obtain biodegradable regenerated fibroin fiber.DESIGN: A single sample experiment.SETTING: Jiangsu Provincial Key Laboratory of Silk Project of Material Engineering College of Soochow University.MATERIALS: Leftover silk pieces were provided by Suzhou Silk Import & Export Corp.METHODS: This study was performed at Jiangsu Provincial Key Laboratory of Silk Project of Material Engineering College of Suzhou University between January 2003 and December 2004. By controlling the molecular of abandoned natural fibroin fiber during neutral salt dissolution process, wet spinning technique and draft method were applied to spin regenerated fibroin fibers that have certain mechanical and biodegradable properties.The specific procedure is as follows: ①fibroin preparations. ② Spinning solution preparations. ③ Regenerated fibroin fibers were spun with wet spinning method. ④Sodium lauryl sulphate-polyacrylamide gel electrophoresis method was used to detect the molecular weight of LiBr dissolved fibroin solution. ⑤To detect the crystallinity and orientation of fibroin fibers. ⑥ To detect the mechanic properties of regenerated fibroin fibers. ⑦ To detect the in vitro enzyme degradation rate of fibroin fibers.MAIN OUTCOME MEASURES: ①The relative molecular weight of LiBr dissolved silk fibroin. ② Result of X-Ray diffraction. ③The in vitro enzyme degradation rate of regenerated fibroin fibers.RESULTS: ①After LiBr dissolution, the molecular weight of fibroin spinning solution was mainly below 100 000. ②During the coagulation and pulling process of regenerated fibroin fiber wet spinning, fibroin fiber conformation changed from random convolution to the co-existence of β-folding and random coi1/α-spiral conformation in molecular chain. ③ Actinozyme was used to assess the in vitro enzyme degradation rate of hexafluoroisopropanol spun regenerated silk fibroin, and the 30 day-degradation rate of regenerated fibroin fibers was 37.16%, compared with 10.70% of natural fibroin fiber.CONCLUSION: Wet spinning technique and draft method can be used to spin regenerated fibroin fibers possessing certain mechanical and biodegradable properties by controlling the molecular weight of abandoned natural fibroin fibers during neutral salt dissolution process.