ABSTRACT
<p><b>AIM</b>For further investigating of heat shock transcription factor 1 (HSF1) action in thermoregulation and its physiological mechanism.</p><p><b>METHODS</b>The relationship among the expression of HSF1 and interleukin 1beta (IL-1beta) mRNA and tumor necrosis factor alpha (TNF-alpha) mRNA in monocytes was studied during the different fever stages in rabbit fever model induced by LPS. The expressions of IL-1beta and TNF-alpha mRNA were measured by RT-PCR assay; HSF1 expression was measured by Western blot.</p><p><b>RESULTS</b>(1) Intravenous injection of LPS produced a double-peak temperature arisen at 60 min and 180 min. (2) The expression of IL-1beta mRNA in monocytes had a peak at 160 min, while the peak of TNF-alpha mRNA expression occurred at 80 min after intravenous injection of LPS. (3) The content of the HSF1 trimer increased gradually after 160 min intravenous injection of LPS. The results indicated that the content of the HSF1 trimer was negative correlation with the expressions of IL-1beta mRNA and TNF-alpha mRNA, and the change in body temperature was a positive correlation with IL-1beta mRNA.</p><p><b>CONCLUSION</b>It is possible that HSF1 limits the rise in body temperature by repressing the gene expressions of the endogenous pyrogen, IL-1beta and TNF-alpha.</p>