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Objective To investigate the inhibitory effect of harmine on osteosarcoma cell proliferation and apoptosis by down regulating cyclooxygenase-2 (COX-2) expression.Methods Human osteosarcoma cell line U2OS was cultured in vitro and randomly divided into control group,study group 1,study group 2 and study group 3.The cells were cultured in 0,5 μmol/L,10 μmol/L and 20 μmol/L concentration of harmine for 48 hours.Cell counting kit-8 (CCK-8) method and flow cytometry were used to detect cell viability and apoptosis.The expression level of COX-2,proliferation and apoptosis related proteins and mRNA were detected by Western blot and real-time quantitative polymerase chain reaction (RT-qPCR),respectively.Results After cultured with different concentrations of harmine for 12,24 hours and 48 hours,the cell viability of the three study groups were significantly lower than that of the control group (P ＜ 0.05),while that of the study groups 2 and 3 were significantly lower than that of the study group 1 (P ＜ 0.05).The apoptosis rate of the three study groups were significantly higher than that of the control group (P ＜0.05),while that of the two groups were significantly higher than that of the study group 1 (P ＜ 0.05).After 48 hours of culture,the levels of COX-2,cyclin D1,proliferating cell nuclear antigen (PCNA),B-cell lymphoma-2 (Bcl-2) protein and mRNA expression in study group 2 were significantly lower than those in control group,while the expression levels of cleaved caspase-3 and BCL2-Associated X (Bax) in study group 2 were significantly higher than those in control group (P ＜ 0.05).Conclusions Harmine can inhibit the proliferation and promote the apoptosis of osteosarcoma cells by inhibiting the expression of COX-2 and regulating the expression of cell cycle and apoptosis related protein.
ABSTRACT
Objective@#To understand the relationship between perceived social support, psychological capital and job satisfaction, and analyze the mediating effect of psychological capital between perceived social support and job satisfaction.@*Methods@#In 2017, June to July, we use cluster random sampling method to evaluate 888 medical staff from 5 hospitals in Jinan and Yantai in Shandong provience by Perceived Social Support Scale、PsyCap Questionnaire and MinnesotaSatisfaction Questionnaire.@*Results@#Correlation analysis showed that perceived social support was significantly positively correlated with self-efficacy, hope, resilience, optimism and job satisfaction (r=0.42, 0.42, 0.56, 0.47, P<0.05) . There was a significant positive correlation between job satisfaction and self-efficacy, hope, resilience and optimism (r=0.45, 0.50, 0.43, 0.46, P< 0.05) . The structural equation model shows that hope and optimism play a significant partly mediating effect between perceived social support and job satisfaction.@*Conclusion@#Perceived social support is a positively predictive factor of job satisfaction in medical staff. The Increase of perceived social support and psychological capital are conducive to improving job satisfaction of medical staff's.