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BACKGROUND:A large amount of inflammatory mediators from myocardial cels are secreted in response to myocardial injury after myocardial ischemia. Moreover, inflammatory cytokines in infarction and ischemia regions contribute to myocardial tissue repair and adaptation. OBJECTIVE:To investigate the effect of tumor necrosis factor-α (TNF-α) pretreatment on cardiac function of myocardial infarction rabbits undergoing umbilical cord blood mesenchymal stem cel transplantation. METHODS: Thirty-six white rabbits were equally randomized into sham, model, non-TNF-α, and TNF-αgroups. Animal models of myocardial infarction were made in the latter three groups. Twenty-four hours after modeling, PBS, umbilical cord blood mesenchymal stem cells pretreated with or without TNF-α were injected at infarct center and border, respectively, in the model, TNF-α and non-TNF-α groups. RESULTS AND CONCLUSION: Model rabbits in the TNF-α and non-TNF-α groups showed better cardiac function and lower size of myocardial infarction and fibrosis than those in the model group. Compared with the non-TNF-α group, moreover, the TNF-α group showed better outcomes in these indicators. These findings indicate that TNF-α pretreatment canmarkedly improve the therapeutic efficacy of umbilical cord blood mesenchymal stem cel transplantation on myocardial infarction.
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BACKGROUND:Stem cels have multi-directional differentiation and self-replication abilities, under certain conditions, which can differentiate into myocardial cels to repair the damaged myocardium. OBJECTIVE: To investigate the effects of transplantation of umbilical cord mesenchymal stem cels derived at different gestational weeks on infarct size and angiogenesis in the infarct region of experimental rabbits with myocardial infarction. METHODS: Ten ful-term umbilical cord samples and 10 umbilical cord samples of aborted fetuses at 10-12 gestation weeks were selected to in vitro isolate umbilical cord mesenchymal stem cels that were subjected to BrdU labeling. HLA-G expression was detected in the cels. Thirty white rabbits were selected to make myocardial infarction models, and 2 weeks after modeling, the model rabbits were randomized into aborted cel transplantation group, ful-term cel transplantation group and control group (n=10 per group). Then, BrdU-labeled cels were injected correspondingly into the infarct region of rabbits in the two cel transplantation groups. Rabbits in the control group were subjected to an equal volume of serum-free. Four weeks after transplantation, heart function of rabbits was monitored using electrocardiogram, and myocardial tissues were taken to measure infarct size and blood capilary density. RESULTS AND CONCLUSION: HLA-G expression was different in different sources of umbilical cord mesenchymal stem cels: high HLA-G expression was found in the aborted umbilical cord mesenchymal stem cels, and meanwhile, low HLA-G expression was found in the ful-term umbilical cord mesenchymal stem cels. Compared with the control group, the left ventricular end-diastolic volume and left ventricular ejection fraction of aborted and ful-term cel transplantation groups were significantly improved, especialy in the aborted cel transplantation group (P < 0.05). BrdU-positive cels were found in the infarct site in both transplantation groups. Compared with the control group, the infarct size and capilary density were improved most significantly in the aborted cel transplantation group folowed by the ful-term cel transplantation group (P < 0.05). Electrocardiogram findings showed significant improvement in both cel transplantation groups compared with the control group (P < 0.05), especialy in the aborted cel transplantation group. These findings indicate that umbilical cord mesenchymal stem cels derived at low gestational weeks improve the heart function more significantly than the ful-term umbilical cord mesenchymal stem cels, which have the potential to become a better source of cardiomyocytes for transplantation.
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BACKGROUND:Human umbilical cord blood-derived mesenchymal stem cels are able to repair and regenerate the injured myocardium, which is a new therapy for myocardial infarctionvia transplantation. OBJECTIVE:To explore the therapeutic efficacy of intracoronary injection of human umbilical cord blood-derived mesenchymal stem cels on acute myocardial infarction in rats. METHODS:Thirty-two rats were selected to make animal models of ligation of the left anterior descending coronary, and then model rats were randomized equaly to transplantation group and model group. Human umbilical cord blood-derived mesenchymal stem cels were isolated and prepared into cel suspension. Rats in the transplantation group were subjected to transplantation of human umbilical cord blood-derived mesenchymal stem cels. RESULTS AND CONCLUSION: Human umbilical cord blood-derived mesenchymal stem cels were successfuly isolated and cultured in vitro. Compared with the model group, the microvessel density, left ventricular end-systolic pressure and ±dp/dtmax were significantly increased in the transplantation group (P < 0.05), while the left ventricular end-diastolic pressure was decreased dramaticaly (P < 0.05). Electrocardiography findings showed that the heart function of rats in the transplantation group was improved slightly. These findings indicate that human umbilical cord blood-derived mesenchymal stem cels can promote myocardial angiogenesis and improve heart function of rats with myocardial infarction.
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Objective By investigating the departments of ECG and the staff in China,to study the current ECG status and thus provide references for training ECG workers,improving the work environment and stabilizing the ECG professional team.Methods One hundred and twenty four domestie grass-root hospitals were investigated through real-name questionnaire surveys.The data was collected and analyzed.Results Among the 124 investigated hospitals,the educational background description is:(1) The ECG workers in provincial or municipal hospitals had mainly been entitled with Bachelor's degree or above accounting for 72.7％ of whole ECG workers;the ECG workers in the county or township hospitals were entitled with college degree or below,accounting for 85.7％ of whole ECG workers.The difference between the two kind of workers was significant(P＜0.01).(2) The proportion of ECG workers owning senior professional titles in provincial or municipal hospitals and county or township hospitals were both low,which were 12.2％ and 3.6％,and the former one was significantly higher than the later(P＜0.01).(3) The proportion of hospitals which established dependent department of ECG was low;the percentage of provincial or municipal hospitals was 23.4％,which was significantly higher than that of county or township hospitals (3.3％,P＜0.05).(4) The provincial or municipal hospitals were well equipped and also conducted various of inspection projects in comparison to county or township hospitals.Conclusions The grass-root ECG staff in China has no satisfying education background and professional titles;ECG department belonging varies and the special inspection projects are insufficient and the economic benefit was low.So this study suggest that the competent departments take measures in improving ECG personnel enthusiasm,improving the working environment,and ensuring the healthy and orderly development of ECG profession.
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<p><b>OBJECTIVE</b>To explore the characteristics of RR-Lorenz plot in persistent atrial fibrillation (AF) patients complicating with escape beats and rhythm though ambulatory electrocardiogram.</p><p><b>METHODS</b>The 24-hour ambulatory electrocardiogram of 291 persistent AF patients in second affiliated hospital of Zhengzhou university from July 2005 to April 2013 were retrospectively analyzed and the RR interval and the QRS wave were measured. Patients were divided into two groups according to the distribution of the RR-Lorenz point [AF without escape beats and rhythm group (Group A, n = 259) and AF with escape beats and rhythm group (Group B, n = 32)]. The characteristics of RR-Lorenz plot between the two groups were compared.</p><p><b>RESULTS</b>(1) Fan-shaped RR-Lorenz plots were evidenced in Group A. (2)In Group B, 30 cases showed fan-shaped with L-shaped and a short dense rods along 45° line. The proportion of escape beats and rhythm was 0.28% (275/98 369) -14.06% (11 263/80 112) . The other 2 cases in group B showed no typical RR-Lorenz plots features.</p><p><b>CONCLUSION</b>RR-Lorenz plot could help to quickly diagnose persistent AF complicating with escape beats and rhythm according to the typical RR-Lorenz plot characteristics in 24-hour ambulatory electrocardiogram.</p>
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Aged , Aged, 80 and over , Female , Humans , Male , Atrial Fibrillation , Electrocardiography, Ambulatory , Retrospective StudiesABSTRACT
Objective To investigate health literacy before and after the medical intervention and its influencing factors so as to provide evidence for public health promotion.Methods Stratified multi-stage random sampling method was applied for residents aged 15-69 years from 8 urban or rural communities of Taishun County of Lucheng District.The comprehensive health literacy promotion and intervention was carried out,and a unified citizen health literacy questionnaire was designed.Residents' health literacy,the three aspects of health literature and five categories of health literacy issues levels were compared before and after the intervention,and Chi-square test was used for data analysis.Results The overall rate of having health literacy was 11.8％ and 16.3％ in 2012 and 2013,respectively (x2=7.20,P＜ 0.01).The rate of basic health concept and knowledge,health lifestyles and behaviors,and medical skills were significantly raised from 18.5％,9.4％ and 23.5％ to 28.0％,14.6％ and 33.5％,respectively (x2 values were 21.60,11.07 and 20.85,respectively; all P＜0.01).As to the five issues of health literacy,the level of the scientific healthy literacy,the chronic disease literacy,the literacy of infectious disease,the safety and first aid literacy and the basic health literacy were significantly inclined (52.4％ vs.40.4％,39.3％ vs.31.8％,27.8％ vs.22.4％,74.0 ％ vs.58.2％ and 41.9％ vs.33.8％,respectively; x2 values were 27.79,34.95,8.10,43.07 and 13.61,respectively; all P＜0.01).Conclusion Health education was helpful in the promotion of healthy literacy.Healthy lifestyles and behavior literacy and chronic disease prevention literacy should be important for the rural,elderly,low-education level and low-income populations.
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ObjectiveTo study the Electrocardiogram (ECG) changes in AIDS patients at different age grades. MethodsThe ECG of 400 AIDS patients at different age were analyzed retrospectively. Results(①)The rate of abnormal ECG in the age group of 46 ～50 years was significantly higher than 11 ～ 15(P =0. 008) ,21 ～25( P = 0. 041 ),31 ～ 35 ( P = 0. 022 ),41 ～ 45 ( P = 0. 001 ) and 51 ～ 55 ( P = 0. 047 ) years groups respectively. (②)The rate of bradyarrhythmia in the age group of 46 ～ 50 years was significantly higher than 31 ～ 35 (U = 2. 44) ,36 -40( U = 2. 18 ) ,41 ～ 45 ( U = 2. 57 ) years groups ( P ＜ 0. 05 respectively. (③)The rate of left atrial and ventricular hypertrophy in 11 ～ 15 years group was significantly lower than the age groups older than 46 (except for 51 ～ 55years group) ;those aged ＞60 had higher atrial and ventricular hypertrophy rate than 36 ～40,41 ～45 and 51 ～55years groups ( P ＜ 0. 05 respectively). ConclusionsAIDS patients at all ages may present abnormal ECG, which is positively correlated with age.
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As a special profession in our society,doctor has its special rights.An investigation of Jinzhou,Chaoyang,Huludao,Panjin,Fuxin and Tieling,Anshan in the Liaoning province review that violation of doctors' right occurs from time to time.There are several reasons for the violation,mainly coming from doctors,patients and their relatives,guidance of social media and social causes in depth.