ABSTRACT
OBJECTIVE:To develop an RP-HPLC method for the determination of Paeoniflorin in Xuefu zhuyu liquor.METHODS:A SunFireTM C18(250 mm?4.6 mm,5 ?m) column was used with the mobile phase consisted of acetonitrile-1% glacial acetic acid-methanol(5∶24∶71) at a flow rate of 1.0 mL?min-1.The temperature of column was 25 ℃ and the dete-ction wavelength was set at 240 nm.RESULTS:Paeoniflorin showed a good linear relationship at a range of 0.038 4～0.307 2mg?mL-1(r=0.999 6),and the average recovery rate was 100.26%(RSD=1.36%,n=9).CONCLUSION:The method is simple and accurate,and it can be used for the quality control of Xuefu zhuyu liquor.
ABSTRACT
AIM: To establish the quality standard of Shajun Zhiyang Lotion (Radix Sophorae Flavescentis, Cortex Phellodendri Chinensis, Fructus Cnidii, Borneolum Syntheticum, etc.). METHODS: Cortex Phellodendri Chinensis and Fructus Cnidii and Borneolum Syntheticum were identified by TLC. The content of matrine was determined by HPLC. RESULTS: TLC identification was highly specific and the sports were clear. The linear range for matrine was in the range of 0.295 3-2.362 0 ?g and its average recovery was 100.38% and RSD was 1.5%, respectively. CONCLUSION: The quality of Shajun Zhiyang Lotion could be controlled effectively according to the quality standard.