Synthesis and antitumor activity of 13-acylmatrine derivatives / 药学实践杂志

Objective To synthesize a series of 13-acylmatrine derivatives and evaluate their in vitro antitumor activity . Methods Using sophocarpine as the starting material ,a series of new compounds were synthesized through Michael addition , Staudinger reduction and acylation .The structure of target compounds were confirmed by 1 H NMR and MS techniques .Their antitumoractivityagainsthumanhepatomacells(BEL-7404)andmicemelanomacells(K111)wereevaluated invitrobyMTT assay .Results We synthesized 9 compounds and all the compounds exhibited inhibitory activities against BEL-7404 and K111 . Conclusion Compound 4b and compound 4e exhibit good in vitro antitumor activity to human hepatoma cells (BEL-7404) .

Influencing factors and clinical significance of metastatic lymph node staging in advanced gastric carcinoma / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigative the influencing factors of metastatic lymph node staging (N staging), and the effect of number of lymph node dissection on the prognosis in advanced gastric carcinoma.</p><p><b>METHODS</b>Clinicopathological data of 395 advanced gastric cancer patients undergoing radical operation in Harbin Medical University Cancer Hospital in 2011 were retrospectively analyzed. Logistic regression was used to investigate the influencing factors. Cox model was used to evaluate the prognostic factors. Association of survival with the number of lymph node dissection in different N stage was further examined.</p><p><b>RESULTS</b>Lymph node metastasis was found in 307 patients(77.7%), including 88 of N0, 86 of N1, 111 of N2 and 110 of N3. Overall 3-year survival rate was 65.3%. Borrmann classification (χ(2)=32.045, P=0.000), histopathological type (χ(2)=5.595, P=0.018), depth of invasion (χ(2)=27.227, P=0.000) and the number of lymph node dissection (χ(2)=12.337, P=0.000) were influencing factors of metastatic lymph node. Tumor location(OR=2.86, 95% CI: 1.80~4.53, P=0.000), depth of invasion (OR=1.44, 95% CI: 1.12~1.85, P=0.004) and the number of metastatic lymph node (OR=1.58, 95% CI: 1.33~1.87, P=0.000) were independent prognostic factors(all P < 0.05), and number of lymph node dissection was not associated with prognosis (P > 0.05). Subgroup analysis revealed overall survival rate of stage N3 patients with ≥ 40 of lymph node dissection was obviously higher as compared to those with < 40, while such difference was not found in N0, N1, N2 groups (all P > 0.05).</p><p><b>CONCLUSIONS</b>Lymph node staging of advanced gastric cancer is associated with Borrmann classification, histopathological type, depth of invasion and number of lymph node dissection. Dissection of 20 lymph nodes is suitable for stage N0, N1 and N2, while ≥ 40 lymph nodes should be dissected in stage N3.</p>

Prognostic value of preoperative neutrophil-to-lymphocyte ratio in the elderly patients over 75 years old with gastric cancer / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the clinical implication of preoperative neutrophil-to-lymphocyte ratio (NLR), and association of NLR with the prognosis of the elderly patients over 75 years old with primary gastric cancer.</p><p><b>METHODS</b>Clinical data of 160 patients (≥75 years) with gastric cancer undergoing gastrectomy in Department of Gastrointestinal Surgery, the Tumour Hsopital of Harbin Medical University form January 2007 to December 2010 were retrospectively analyzed. Preoperative neutrophil and lymphocyte count was measured and NLR was calculated. The cut-off value of NLR to predict the survival was obtained from the receiver operating characteristic(ROC) curve. Patients were divided into two groups based on cut-off value. Clinicopathological features were compared between two groups using Chi-square test or Fisher exact test. Cox proportional hazard model was used to analyze risk factors associated with survival.</p><p><b>RESULTS</b>The cut-off value of NLR was 1.83 with 0.709 of sensitivity and 0.562 of specificity. A total of 54 patients with NLR<1.83 belonged to NLR-0 group, and 106 patients with NLR≥1.83 belonged to NLR-1 group. As compared to NLR-0 group, patients in NLR-1 group had significantly higher proportion in maximum tumor size ≥ 50 mm [66.0%(70/106) vs. 42.6%(23/54), P=0.004], serosal invasion [75.5%(80/106) vs. 57.4%(31/54), P=0.029], positive lymph node metastasis [83.0% (88/106) vs. 55.6%(30/54), P=0.001] and TNM stage III( [79.2%(84/106) vs. 61.1%(33/54), P=0.013]. The median survival of NLR-0 and NLR-1 group was 1 209 days and 587 days respectively, with significant difference(P=0.001). Multivariate analysis showed that NLR≥1.83(HR=0.530, 95% CI: 0.332 to 0.846, P=0.008), serosal invasion (HR=0.570, 95% CI: 0.332 to 0.979, P=0.042), and lymph node metastasis(HR=0.475, 95% CI: 0.462 to 1.685, P=0.033) were independent risk factors of poor prognosis(all P<0.05).</p><p><b>CONCLUSION</b>Preoperative higher NLR value in the elderly patients over 75 years old with primary gastric cancer indicates larger tumor size, severe serous invasion, more lymph node metastasis, later TNM staging, and poorer prognosis.</p>

Synthesis and antifungal activities of novel triazole derivatives containing sulfur atoms / 药学实践杂志

Objective To design and synthesis novel triazole antifungal derivatives with dithiocarbamates side chain and study the antifungal activities .Methods Nine title compounds were synthesized and characterized by 1 H NMR ,MS spectra . The in vitro antifungal activities of all the compounds were evaluated against eight human pathogenic fungi .Results The title compounds exhibited strong antifungal activities .Some compounds showed excellent activities against Candida albicans with the MIC80 values less than 0 .125 g/ml ,6d was 64 times higher than that of Itraconazole .Conclusion The introduction of the propyl ,sulphur and benzyl moiety to the side chain could affect the antifungal activities .

Research of Hip Angle in Hemiplegic Gait / 中国康复理论与实践

@# Objective To study the hip angle characteristics in hemiplegic gait.Methods Gait analysis was performed in 20 subjects with hemiplegia and 20 healthy subjects and the hip joint angle of all subjects was analyzed.Results The hip joint angle of affected-side lower limb of hemiplegic gait showed significant differences between 20 subjects with hemiplegia and 20 healthy subjects on hip flextion at heel-contact,maximum extension in stand phase,flextion toe-off,maximum flexion in swing phase and total excursion on sagittal plane.Conclusion The research on the characteristics of hemiplegic gait is helpful to make clinical assessment and treatment planning.

Reliability of the gait analysis system based on digital video and image processing in the assessment of gait / 中国康复理论与实践

@#ObjectiveTo study the reliability and sensitivity of the gait analysis system based on digital video and image processing.Methods20 healthy subjects were assessed twice by the same doctor having one-week interval to investigate intraobserver reliability, they were also assessed twice separately by two doctors to investigate interobserver reliability. Gait parameters of affected limb of 20 hemiplegic patients were compared with 20 healthy subjects to investigate the sensitivity of this system.ResultsThere were high correlations in all the parameters between the two assessments. The parameters of the patients were significantly different from those of the controls.ConclusionThe system is sufficiently repeatable and sensitive in the assessment of gait.

Algorithm for recognizing the markers in human motion detection / 生物医学工程学杂志

An algorithm for recognizing the markers in human motion detection is proposed in this paper. It includes three parts as follows: (1) an improved template matching method is used to acuminate the peak of the matching result. (2) The weighted average of the cross correlation between target image and template is given in order to make the resolution increase to sub-pixel level. (3) Forecasting the motion of marker and overcoming the hiding of marker are realized basing on the continuity of human motion. The result of experiments shows that the algorithm can recognize the positions of the markers at a relatively higher accuracy and speed. A gait analysis system based on this algorithm has come into use.

A study of gait recognition based on kinematics and kinetics parameters / 生物医学工程学杂志

In order to recognize people by their gait, we propose a pattern recognizing method based on kinematics and kinetics parameters. The feature extraction methods of joint angle and vertical ground reaction force (VGRF) were given. 14 healthy male subjects participated in this experiment. The experimental results showed that the correct classification rates (CCR) was 87.1% at k = 1 and 90% at k = 3 (k-nearest neighbor) based on joint angle recognition; the CCR was 85.7% at k = 1 and 80% at k = 3 based on VGRF recognition. The multivariate analysis of the experimental data proved the feasibility of gait recognition. The principal component analysis and curves of VGRF also showed that the instant of foot strike plays an important role in gait recognition.