ABSTRACT
Objective To study the clinical effect of terbutaline combined with budesonide suspension liquid in the treatment of children with chronic bronchitis .Methods A total of 116 children with chronic bronchitis were selected,and they were randomly divided into the control group and the observation group according to the digital table,58 cases in each group .Based on symptomatic treatment ,the control group was given budesonide treatment ,the observation group was treated with terbutaline combined with budesonide treatment,All the patients were treated for 7 days.The curative effect,clinical symptoms disappeared time and adverse reaction etc.were compared between the two groups.Results After treatment,the total effective rate of the observation group was 94.8％,which of the control group was 81.0％,the difference was statistically significant (χ2 =5.599,P<0.05).The cough disappeared time , wheezing disappearance time , rales disappearance time and wheeze disappeared time of the observation group were (5.1 ±1.4)d,(3.5 ±0.8)d,(3.9 ±1.1)d,(4.4 ±0.9)d,respectively,which of the control group were (8.2 ± 2.1) d,(4.7 ±1.2) d,(5.4 ±1.3) d,(7.1 ±1.4) d,respectively,the differences between the two groups were statistically significant(t=9.354,6.337,6.708,12.355,all P<0.05).The incidence rate of adverse reaction of the control group was 15.5％,which of the observation group was 13.8％,there was no statistically significant difference between the two groups(χ2 =0.034,P>0.05).Conclusion Terbutaline combined with budesonide suspension in the treatment of children with chronic bronchitis can significantly improve clinical symptoms ,improve clinical curative effect,and with good safety and great clinical significance .
ABSTRACT
Objective To investigate the clinical efficacy of inhalation of terbutaline,budesonide combined with Xiaoerkechuanling granules in the treatment of infantile asthma.Methods 96 children with asthma were selected and randomly divided to observation group and control group.The two groups were given routine drug treatment,the control group was given sulfate inhalation terbutaline atomization liquid and budesonide suspension on the basis of conventional treatment.The observation group was given Xiaoerkechuanling granules on the basis of the control group. The two groups were treated for 7 days.The clinical curative effect of the two groups,the changes of lung function and adverse reactions were observed.Results After treatment,the total effective rate in the observation group was 93.80%,which in the control group was 75.00%,the difference between the two groups was statistically significant (χ2 =3.200,P 0.05 ).Conclusion Inhalation of terbutaline,budesonide combined with Xiaoerkechuanling granules for the treatment of asthma in children can significantly improve the patients'pulmonary function,improve clinical curative effect,and it has less adverse reaction and great clinical value.
ABSTRACT
Objective To study the mechanism of U937 cell (human histocytic lymphema) apoptosis induced by ATP. Methods ATP (0^23?g/L) was added into U937 cell cultured medium as experiment group, no ATP adding group as the control. Using immunofluorescent cytochemistry method to demonstrat the expresses of Cx43, F\|actin, vinculin. Results ATP induced U937 cell to form apoptotic bodies. Expresses of Cx43, F\|actin, vinculin were increased in ATP treated U937 cells.Conclusion ATP could induce U937 cell apoptosis. Apoptotic bodies formed from ATP treated U937 cells was correlated closely with express of Cx43, F\|actin, vinculin, It indicats that Cx43, F\|actin, vinculin play important roles in U937 cell apoptosis induced by ATP.
ABSTRACT
This study was carried out to investigate the effects of ATP on cultured human stomach carcinoma MGC-803 cells. After ATP(0.16—0.23 mg/ml, 24—120 hrs)treatment, inhibition of cell growth rate was observed. The inhibition rate reached 80% after 96 hr exposure to ATP. At the same time, anti-tubulin immunofluorescent staining showed increase of organized microtubules (MT) in ATP-treated MGC-803 cells, in contrast with the poor MT immunofluorescence in control MGC-803 cells. By using rhodamie phalloidin combined staining, increased number of actin bundles, took the place of actin dots and patches which were seen in control MGC-803 cells. Immunofluorescent staining of fibronectin in ATP-treated MGC-803 cells was also increased evidently as compared with the control MGC-803 cells. Concomitant with the improvement of MT and stress fiber organization, the cell Concohas become more flattened. It appeared that the increase of MT and stress fibers was closely related to the recovery of cell morphology in ATP treated tumor cells. It is therefore concluded that ATP not only may inhibit tumor cell proliferation, but also may induce differentiation of cell morphology of tumor cells through its action on cytoskeletal structures.
ABSTRACT
This experiment was carried out in young adult albino mice,which were injectedwith Ehrlich ascites tumor cells intraperitoneally to produce Ehrlich ascites tumor.The animals were divided into 2 groups.The first group received cAMP plusaminophylline for 5 to 13 days after inoculation.The second group received saline ascontrol.We found that in the administration of cAMP together with aminophylline,thegrowth of Ehrlich ascites tumor cells was inhibited to the extent of 53% at the 9thdays after inoculation.Early or later than the 9th day,the inhibitory rates were marklylower.By using cAMP immunocytochemical method,it was found that on the9th day of inoculation,there was an increase of the intensity of intracellualr cAMPspecific fluorescence in tumor cells of the cAMP treated mice in comparison withthose of the control.It also inhibited the 3',5'-cAMP-PDE activity on the 5th to 7thday after inoculation.Under the dark field microscope on the surface of the Ehrlich tumor cells therewere numerous“brush-like”microvilli,but they were not visible on the surfaceafter treated with cAMP together with aminophylline.The agglutination by theCon.A,was decreased markly on the 7th to 9th day after the inoculation.The possible relationship between the level of intracellular cAMP and of the3',5'-cAMP-PDE,and especially the inhibition of the formation of microvilli onthe treated tumor cell surface is discussed in this paper.