ABSTRACT
Objective:To confirm the mechanism of Sedum alfredii extract (SafE) alleviating radiation injury in human small intestinal epithelial cells (HIEC-6). Methods:HIEC-6 cells were divided into 4 groups, including control group (Con), irradiation group (IR), SafE alone group (SafE) and SafE plus irradiation group (SafE+ IR). All of the SafE groups were treated with 0.02 g/ml (W/V) SafE for 24 h. Cell viability (CCK-8 method ) and intracellular ROS levels were investigated at 24 h after 2, 4, and 6 Gy irradiation. Samples were taken at 24 h after 4 Gy irradiation for transcriptome analysis, and the intracellular E3 ubiquitin ligase PRKN expression level was measured. The thickness of endoplasmic reticulum was detected at 24 h after 4 Gy irradiation using fluorescent dye.Results:SafE could maintain cell viability after irradiation ( t=2.94-10.40, P<0.05), and significantly reduced the level of ROS in the irradiated cells ( t=-13.29--4.53, P<0.05). PRKN was preliminarily verified to be the target gene of SafE that maintained PRKN transcript level and endoplasmic reticulum thickness after irradiation (IR group vs. Con group: t=-5.55, 3.27, P<0.05, SafE group vs. SafE+ IR group: P>0.05). Conclusion:SafE is effective in maintaining ER thickness and reducing cellular radiation damage and its target gene PRKN could be regulated by ionizing radiation.
ABSTRACT
Objective To evaluate the value of MSCT and MRI in diagnosis of early postoperative complications of inguinal hernia repair (IHR).Methods Imaging and clinical data in 1 6 patients with early complications of IHR were analyzed retrospectively.Re-sults Among the complications,postoperative infection in 2 was found including incision infection in 1 and groin cellulitis in other 1. CT showed swelling abdominal wall and heterogeneous enhancement for incision infection,and inguinal mass,deep inguinal ring thickening,edema of residual sac with fluid and air collections for groin cellulitis.Seroma was found in 8,and CT and MRI demon-strated residual sac effusion,spermatic cord thickening and spermatic vascular tortuosity.Residual sac edema was found in 4,and CT and MRI showed thickening spermatic cord without effusion in residual sac or scrotum.Effusions between patch and anterior abdom-inal wall were detected by MRI in 2,one of which was accompanied by patch shrinking.Conclusion With specific clinical background for early complications of IHR,MSCT and MRI provide more anatomical information of inguinal region,which may contribute to di-agnosis and treatment of early postoperative complications.