ABSTRACT
Objective To analyze the characteristics of 5 152 cases HPV genotyping in Guangxi province ,which will be benefit for the control of HPV infection and provid experimental evidence for clinical treatment .Methods Statistically analyze the positive detection rate of all the HPV subtypes ,the differences in the positive rate between people of different genders and ages .Results The total positive rate was 37 .46% (1 930/5 152) .The subtypes with the top seven positive rates were HPV16 (5 .90% ) ,HPV52 (5 .36% ) ,HPV58(4 .04% ) ,HPV6(3 .40% ) ,HPV53(2 .66% ) ,HPV11(2 .43% ) ,HPV18(2 .19% ) ,which were mainly high‐risk subtypes .The total positive rate of male patients was 87 .71% ,while female patients was 34 .45% ,the total positive rate of male pa‐tiets was higher than women .For the positive rate of HPV6 ,HPV11 and HPV58 ,male patients were higher than women ,while for HPV52 female patients was higher than men(P<0 .05) .High‐risk HPV6 ,HPV11 ,HPV42 ,HPV43 infection were characterized by the tendency of younger patients ,the differences were statistically significant(P<0 .05) ,the positive infection rate of patients equal or less than 20 years old(75 .51% ) was higher than other age groups .Conclusion HPV infection rates are very high in some re‐gions of Guangxi ,and attention should be paid to male HPV infection .The subtypes with the top seven positive infection rate are mainly high‐risk subtypes .Low‐risk subtypes such as HPV6 ,11 ,42 ,43 are characterized by the tendency of younger patients .The distribution of HPV infection was affected by region ,gender and age .The investigation of HPV subtypes in Guangxi and do HPV screening in different age groups could help the prevention of cervical cancer and understanding HPV infection outcome .
ABSTRACT
<p><b>OBJECTIVE</b>To screen rare blood groups Fy(a-), s-, k-, Di(b-) and Js(b-) in an ethnic Zhuang population.</p><p><b>METHODS</b>Sequence-specific primers were designed based on single nucleotide polymorphism (SNP) sites of blood group antigens Fy(b) and s. A specific multiplex PCR system I was established. Multiplex PCR system II was applied to detect alleles antigens Di(b), k, Js(b)1910 and Js(b) 2019 at the same time. The two systems was were used to screen for rare blood group antigens in 4490 randomly selected healthy donors of Guangxi Zhuang ethnic origin.</p><p><b>RESULTS</b>We successfully made the multiplex PCR system I. We detected the rare blood group antigens using the two PCR system. There are five Fy(a-), three s(-), two Di(b-) in 4490 Guangxi zhuang random samples. The multiplex PCR system I has achieved good accuracy and stability. With multiplex PCR systems I and II, 4490 samples were screened. Five Fy(a-), three s(-) and two Di(b-) samples were discovered.</p><p><b>CONCLUSION</b>Multiplex PCR is an effective methods, which can be used for high throughput screening of rare blood groups. The rare blood types of Guangxi Zhuang ethnic origin obtained through the screening can provide valuable information for compatible blood transfusion. Through screening we obtained precious rare blood type materials which can be used to improve the capability of compatible infusion and reduce the transfusion reactions.</p>