ABSTRACT
Objective: To investigate the anti-inflammatory activity of Sang Ju Yin (Mulberry Leaf and Chrysanthemum Decoction), and elucidate its bioactive components on nuclear factor-κappa B (NF-κB) inhibition, and to further clarify the anti-inflammatory mechanism of Sang Ju Yin, so as to provide the basis for establishing its quality standard. Methods: UPLC-Q/TOF MS coupled with NF-κB activity luciferase reporter assay system was applied to determine the potential anti-inflammatory components in Sang Ju Yin. And the human bronchial epithelial cells (BEAS-2B) were selected to set up the inflammatory injury model, caffeic acid, chlorogenic acid, glycyrrhizic acid, loganin, and malonic acid in Sang Ju Yin were investigated for their anti-inflammation. Results: Sixteen components were screened to have the NF-κB inhibitory effects. Among them, malonic acid, 22β-acetoxyl-glycyrrhizic acid, and licorice saponin G2 were first reported to have the NF-κB inhibitory activity. Conclusion: Sang Ju Yin contains the potential NF-κB inhibitors. Sixteen potentially active ingredients are found to have the anti-inflammatory effects as NF-κB inhibitors.