ABSTRACT
OBJECTIVE:To compare therapeutic efficacy and safety of budesonide or beclometasone combined with terbutanline in the treatment of acute attack of mild and moderate asthma in children.METHODS:A total of 110 children with mild and moderate asthma were randomly divided into group A (55 cases) and group B (55 cases).Based on routine treatment,group A was given Terbutaline sulphate solution for nebulization 1 mL,nebulization inhalation,twice a day+Budesonide suspension for inhalation 2 mL,nebulization inhalation,twice a day.Group B was given Terbutaline sulphate solution for nebulization (same usage and dosage as group A)+Beclomethasone dipropionate suspension for inhalation 2 mL,nebulization inhalation,twice a day.Treatment courses of 2 groups lasted for 7 d.Clinical efficacies,symptoms and signs disappearance time,hospitalization time,asthma relief rate as well as symptoms and signs scores,peak value expiration velocity of flow (PEF),the occurrence of ADR were observed in 2 groups.RESULTS:There was no statistical significance in total response rate,symptoms and signs disappearance time,hospitalization time,asthma relief rate or the incidence of ADR between 2 groups (P＞0.05).After treatment,symptoms and signs scores of 2 groups were significantly lower than before,while the PEF were significantly higher than before,with statistical significance (P＜0.05),but there was no statistical significance between 2 groups (P＞0.05).CONCLUSIONS:Based on routine treatment,budesonide or beclometasone combined with terbutaline sulphate can be used for acute attack of mild and moderate asthma in children.They are similar to each other in therapeutic efficacy and safety.
ABSTRACT
A repetitive DNA fragment, named P1, was amplified by PCR with the full-length Minor Ampullate Spidroin gene sequence of Araneus ventricosus as template. P1 was ligated with pPic3.5 and PKT expression vectors and transferred into GS115 and BL21(DE3) competence cells, respectively. SDS-PAGE and Western blot were used to analyze the recombinant his-tag fusion protein. With expressed in different expression systems, soluble P1 induced proteins could be obtained as the same size. Furthermore, the expression level and purification recovery efficiency were also higher in GS115 than that of BL21(DE3). Additionally, the expression level could be improved after optimizing the incubation and induction conditions of GS115. In this research, Pichia pastoris expression system is more suitable for the native repetitive Gly/Ala-rich spider spidroin gene sequence expression than Escherichia coli system. The data can help the native full-length MiSp gene expression and large-scale exploitation of recombinant of spider silk proteins.