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OBJECTIVE： To study the mechanism of Prunus persica-Carthamus tinctorius couplet medicine in the treatment of osteonecrosis of the femoral head （ONFH）. METHODS： The network pharmacology was adopted. The active components of P. persica -C. tinctorius couplet medicine and ONFH target were screened through TCM systematic pharmacological analysis platform target （TCMSP）， DRAR-CPI， hnuman gene database （GeneCards） and online medelian inheritance in man （OMIM） using oral availability of compounds （OB）＞30％ and drug like （DL）＞0.18 as standard. Network topology attribute analysis software Cytoscape 3.6.0 was utilized to construct the active components-ONFH targets network. Target protein interaction network was established on the basis of STRING database， and top 5 target proteins in the list of connectivity were screened， and molecular docking server was used to predict the combination activity of active components from P. persica -C. tinctorius couplet medicine. The biological processes of target gene ontology （GO） and metabolic pathways in Kyoto encyclopedia of genes and genomes （KEGG） were enriched and analyzed by DAVID. RESULTS： A total of 44 active components were screened from P. persica -C. tinctorius couplet medicine， including baicalin， quercetin， etc.， and 78 targets related to ONFH including VEGF， VEGI， CRP， etc. Through analysis of molecular docking server， binding activity of active components of P. persica -C. tinctorius couplet medicine to target protein was strong. GO and KEGG pathway enrichment analysis showed that biological process of P. persica -C. tinctorius couplet medicine for ONFH was related with negative regulation of apoptosis process and positive regulation of nuclear factor-κB transcription factor， mainly through regulating secretory glycoprotein signaling pathway， melanogenesis signaling pathway， VEGF signaling pathway， signaling pathway of basal cell carcinoma， adenosine-activated protein kinase signaling pathway. CONCLUSIONS： This study preliminarily validates the major targets and pathways of P. persica -C. tinctorius couplet medicine for ONFH， which lay a foundation for further study on their pharmacological action.
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BACKGROUND:With the increasing of aging, the incidence and mortality of osteoporotic hip fracture wil rise. It is of great significance to study the pathogenesis and preventing method. At present, finite element analysis can be used to judge fracture, only for the distribution trend of fracture failure in the starting point or section view, but it cannot completely reflect actual situation of fracture. OBJECTIVE:To build the fracture model of the femoral neck fracture caused by fal ing-induced external force based on the finite element analysis LS-DYNA software, and to evaluate the effect of rupture. METHODS:CT image data of one case of elderly femoral neck fracture were col ected. Using Mimics software, region growth of the contralateral area, cavity fil ing, editing, rebuilding the contralateral proximal femur model were conducted. Data were imported in Hypermesh and LS-DYNA software for meshing, and defining material properties. The failure parameters and interfacial properties were set. The load and force boundary constraints simulating the fal ing were simulated. The model of femoral neck fracture was calculated. Rupture effect was evaluated. RESULTS AND CONCLUSION:(1) The validity of contralateral proximal femur three-dimensional model was verified. Based on the finite element analysis software LS-DYNA, the femoral neck fracture model matched the actual fracture line to a degree of close to 83%. (2) Above results confirmed that based on the finite element analysis, LS-DYNA software can wel simulate the femoral neck fracture, which provides experimental basis to the exploration of femoral neck fracture classification mechanism caused by different fal ing-induced external forces.
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@#Objective To investigate the efficacy and analgesia of moxibustion with warming needles combined with joint mobilizationon scapulohumeral periarthritis. Methods 126 subjects were randomly divided into 2 groups, the observation group received moxibustionwith warming needles combined with joint mobilization; the control group received moxibustion with warming needles only. Short-form ofMcGill pain questionnaire and effect were assessed. Results The effective rate was 98.4% and 95.2% for the 2 groups respectively(P>0.05);but there was significant differences between the 2 groups in the visual analogue scale (VAS), present pain intensity (PPI) and pain rating index(PRI) (P<0.05). Conclusion The moxibustion with warming needles combined with joint mobilization is a good effective therapy forscapulohumeral periarthritis.
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@#Objective To investigate the efficacy of facial muscle exercise on peripheral facial paralysis. Methods 45 patients with peripheral facial paralysis were treated with acupuncture, physical therapy and facial muscle exercise. Results The effective rate was 100% and 44.4% were cured after 2 courses of treatment. Conclusion The facial muscle exercise is effective on peripheral facial paralysis.
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Objective To assess neuromuscular electrical stimulation(NMES)for enhancing functional recovery in an upper extremity after stroke.Methods Thirty-seven survivors of a first stroke were randomly divided into a NMES group and a control group.The NMES group(n = 19)received stimulation on the supraspinatus and deltoid muscles and on the wrist extensor using surface electrodes.The stimulation current intensity was set to produce full shoulder and wrist extension with a duty cycle of 5 s on and 5 s off.The stimulus pulses had a symmetric biphasic waveform with a frequency 30 Hz,a pulse width of 300 μs,and amplitudes ranging between 0 to 90 mA.The current amplitude was adjusted according to each subject's sense of comfort.Treatments lasted for 30 min,once daily,5 d per week for 3 weeks.Both groups received the same standard rehabilitation program.Measurements included FuglMeyer motor assessment(FMA)for upper extremity function,somatosensory evoked potential(SEP)for motor function,as well as modified Barthel index(MBI)scores for the activities of daily living.Measurements were recorded before treatment,and after 2 and 3 weeks of treatment.Results Before treatment,there was no significant difference between the two groups in terms of age,stroke severity,or the outcome measures.Significant differences were found in the FMA,MBI and SEP scores of the two groups after 3 weeks of treatment.Conclusion After three weeks of NMES on the affected upper extremity of subjects with early stroke,the patients' motor function had improved.
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Objective:To have an experimental investigation on teaching reform of Orthopedics of TCM based on network resources.Method:Two comparison groups were set and observed when we were teaching the orthopedics students of 2003 grade.One was taught by traditional problem-based learning(PBL) method,the other was taught by PBL method based on network resources.Results:The creative thought and research ability of the students who had been taught by PBL method based on network resources were greatly improved.Conclusion:The teaching method based on network resources has so many advantages such as students being able to self-learn and research without time and space limit that it can be used more in medical students teaching.