ABSTRACT
Objective:To investigate the effects of platelet-rich fibrin extract (PRFe) on the osteogenetic differentiation and mineralization of human periodontal ligament cells (hPDLCs) stimulated by tumor necrosis factor-α (TNF-α) in vitro.Methods:hPDLCs were cultured and identified.PRFe was obtained by Choukroun's protocols.The cells were treated as the 4 groups:① the blank control group,②TNF-α(10 ng/ml),③ PRFe and ④PRFe + TNF-α(10 ng/ml) respectively.Alkaline phosphatase activity was detected by the ALP kit.The alizarin red dye was used to observe the mineralization of the cells.Runx2 and Osterix expression was quantified by Western blotting.Results:The ALP activity,mineralization level and the expression of Runx2 and Osterin of the TNF-oα group were lower that those of the control group(P ＜ 0.05).The examined indexs of PRFe group were higher than that of the control group(P ＜0.05).The indexs of the PRFe +TNF-α group were higher than that of TNF-α group(P ＜0.05).The indexs of PRFe group were higher than that of PRFe + TNF-α group(P ＜ 0.05).Conclusion:PRFe may promote the osteogenetic differentiation of hPDLCs stimulated by TNF-α in vitro.
ABSTRACT
Objective:To study the 3D accuracy of 4 impression materials.Methods:A metal mould with a custom tray was prepared for the measurement.Impression samples of 2 alginate and 2 rubber impression materials were prepared in the metal mould(n =3)re-spectively.The length of three tagged lines,along X,Y,Z axis directions of the samples was measured with a 3D optical scanner.Re-sults:120 min after sampling the variable rates of the length along X,Y,Z axis of Zhermark alginate samples were 3.615%,3.037%and 2.836%,those of CAVEX alginate samples were 2.836%,3.358% and 4.276% respectively;8 h after sampling the variable rates of Impregum Penta polyether rubber samples were 0.039%,0.071% and 0.057%,those of ExpressXT Penta H addition-curing silicone rubber samples were 0.033%,0.088% and 0.084% respectively.Conclusion:The variable rates of the 4 impression materi-als are in accordance with ISO1563∶1990.The variable rate of alginate impression material is greater than that of rubber.