ABSTRACT
Objective@#To determine the value of transoral ultrasound (US) guided biopsy for oral tumors.@*Methods@#A consecutive series of 36 patients who underwent transoral US-guided biopsy of oral tumors were evaluated retrospectively. By testing against histopathological results after surgery, the diagnostic sensitivity, specificity, positive predictive value, negative predictive value and accuracy of transoral US-guided biopsy for oral tumors were calculated. ROC curves were plotted and the area under the curve were evaluated.@*Results@#All 36 lesions were successfully performed transoral US-guided biopsy. The diagnostic sensitivity, specificity, positive predictive value, negative predictive value and accuracy of this technique for the diagnosis of oral lesions were 87.0%, 100%, 100%, 81.3% and 91.7% respectively. In ROC curve analysis, the area under the curve was 0.935. No serious complications were observed.@*Conclusions@#Transoral US-guided biopsy can be considered as a safe and effective technology for early diagnosis and clinical treatment of oral tumors.
ABSTRACT
Objective To determine the value of transoral ultrasound ( US ) guided biopsy for oral tumors . Methods A consecutive series of 36 patients w ho underwent transoral US‐guided biopsy of oral tumors were evaluated retrospectively . By testing against histopathological results after surgery , the diagnostic sensitivity ,specificity ,positive predictive value ,negative predictive value and accuracy of transoral US‐guided biopsy for oral tumors were calculated . ROC curves were plotted and the area under the curve were evaluated . Results All 36 lesions were successfully performed transoral US‐guided biopsy . T he diagnostic sensitivity ,specificity ,positive predictive value ,negative predictive value and accuracy of this technique for the diagnosis of oral lesions were 87 .0% ,100% ,100% ,81 .3% and 91 .7% respectively . In ROC curve analysis , the area under the curve was 0 .935 . No serious complications were observed . Conclusions Transoral US‐guided biopsy can be considered as a safe and effective technology for early diagnosis and clinical treatment of oral tumors .
ABSTRACT
Objective To detect the efficiency of the newly developed PLGA/IO MPs in tracking tendon stem cells (TSCs) by magnetic resonance (MR) and photoacoustic (PA) imaging . Methods Both PLAG/IO MPs and TSCs were prepared and acquired according to the previous study ,and TSCs were incubated with PLGA/IO MPs for labeling .TSCs were collected for MR and PA imaging ,prussian blue staining was performed ,and the iron concentration of labeled TSCs was determined using inductively coupled plasma optical emission spectrometry ( ICP-OES ) at 3 ,7 ,14 ,21 and 28 days after labeling respectively . The rotator cuff injury model was built on the right side of SD rats by surgery and the labeled TSCs were implanted instantly . Dual-modal MR/PA imaging was performed to observe the implanted labeled TSCs at day 3 ,7 ,14 ,21 and 28 after implantation respectively . Results Along with the increase of labeling time ,both MR and PA signal of labeled TSCs decreased gradually ,and the amount of intracellular Fe loading was gradually decreased . At day 28 ,the difference of Fe concentration per cell between labeled TSCs and non-labeled TSCs was not significant (1 .45 pg Fe/cell vs 1 .17 pg Fe/cell , P ＞0 .05) . MR and PA imaging allowed a long-term tracking of labeled TSCs for 21 and 7 days respectively in the rat rotator cuff injury model . Conclusions PLGA/IO MPs are able to label TSCs for up to 21 days ,and dual-modal MR/PA imaging could be used to track the labeled TSCs in the rat rotator cuff injury model .
ABSTRACT
Objective To prepare PLGA microparticles loaded with Iron oxide (PLGA/IO MPs) and explore their feasibility of the rat tendon stem cells (TSCs) labeled with the particles and the multimodal imaging of Ultrasonic (US)/Photoacoustic (PA)/Magnetic resonance (MR) in vitro. Methods The PLGA/IO MPs were prepared using double emulsification,and physical and chemical properties were tested and US/PA/MRI imaging was performed.The TSCs were labeled with PLGA/IO MPs,and transmission electron microscopy (TEM) and prussian blue staining were performed to test labeling effects,then the US, PA and MRI imaging of labeled TSCs were performed. Results The diameter and Zeta potential of prepared PLGA/IO MPs were ( 801.5 ± 165.6) nm and (6.36 ± 3.36) mV [the Zeta potential of microparticles which including poly-L-Lysine(PLL) was about (3.16 ± 3.69)mV],respectively.PLGA/IO MPs could be imaged by US/PA/MRI multimodal imaging. After labeling,the PLGA/IO MPs were distributed in cytoplasm of labeled TSCs which could be imaged by US,PA,MRI simultaneously. Conclusions The TSCs can be labeled with PLGA/IO MPs effectively,and imaged by using multimodal US/PA/MRI imaging in vitro,which will lay foundation for noninvasive and multimodal tracking of transplanted TSCs in vivo.