ABSTRACT
Background: Colorectal cancer [CRC] is the third and a second common cancer in men and women respectively in the world and about 1.4 million new cases diagnosed in 2012. The normal gut microflora consists of bacterial species. One group of them is probiotics, which confer a health benefit to the host. Lactobacillus reuteri [L.reuteri] is known as a probiotic, which lead to the prevention of colorectal cancer. The aim of this study was to assess the inhibitory effects of Lactobacillus reuteri's cell wall on cell proliferation in the colorectal cancer HCT-116 cell line
Materials and Methods: The cells of HCT-116 cell line were grown at 37celsius , 5 percent CO2. L.reuteri was obtained from the Iranian Biological Resource Center and cultured in the MRS Broth at 37 celsius for 48h anaerobically. The cell wall was prepared by the freezing-thawing procedure. So the inhibitory effect of L.reuteri on the growth and proliferation of HCT-116 cells was assessed by MTT assay
Results: The cell wall from L.reuteri inhibited cell proliferation on colorectal cancer HCT-116 cell line. It showed dose- and -time dependent inhibition
Conclusion: These results demonstrated that cell wall of L.reuteri inhibits cell proliferation of HCT-116 cell line
ABSTRACT
Background: Chlamydia trachomatis [C. trachomatis] and Mycoplasma genitalium [M. genitalium] are considered factors in cervical and ovarian cancer and are associated with flaky cell carcinoma of the cervix. The role of steady infection, leading to chronic inflammation, in the of ovarian cancer has received very little consideration, although a background of pelvic inflammatory disease [PID] is in a case-control study associate to higher risk for ovarian cancer. C. trachomatis, the most common and important cause of PID in the developed world is the genital and cervical infectious agent. The aim of this study was prevalence of C. trachomatis and M. genitalium in patients with ovarian cancer who referred to Imam Hossein Hospital of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Materials and Methods: In this descriptive study that was conducted from January 2014 to April 2015, 124 samples were studied which obtained from patients with ovarian cancer who referred to medical centers of Shahid Beheshti University of Medical Sciences. After obtaining samples from ovarian cancer tissue by the pathologist, for extraction DNA, samples were transferred to the laboratory of university. To confirm the presence of C. trachomatis in samples of ovarian cancer, specific primers for the Major Outer Membrane Protein [MOMP] genes of C. trachomais, were designed and used Nested PCR method for detection of M. genitalium. Sequencing was performed on the PCR and Nested PCR product to confirm the presence of C. trachomatis and M. genitalium
Results: Out of 124 samples of ovarian cancer, 62 [50%] samples were malignant cancer and 62 [50%] were benign cancer as control group. From 65 malignant samples 14 [22.5%] were Chlamydia trachomatis positive. None of the tissue samples of benign cancer of ovary were positive for C. trachomatis. Notably, none of the 124 ovarian samples were positive in the M. genitalium standard PCR assay
Conclusion: The results suggest that the spread of C. trachomatis in the female with ovarian cancer may be common. This finding reflects a possible role of C. trachomatis in the carcinogenesis of ovarian tumors. C. trachomatis infection may play a relative role in the pathogenesis of ovarian carcinomas or it could facilitate its progression
Subject(s)
Humans , Female , Adult , Middle Aged , Mycoplasma genitalium , Ovarian Neoplasms/microbiology , Prevalence , Polymerase Chain ReactionABSTRACT
Prostate cancer [PCa] is an important health problem in the aging male population in the world. It is the third most common cancer in the world. Despite of its importance, relatively little is known about its etiology. Sexually transmitted infections [STI] and urogenital pathogens such as Mycoplasma and Ureaplasma, have been proposed as a risk factor for prostate cancer development. This study aimed at detecting the prevalence of Ureaplasma urealyticum [U. urealyticum] and Mycoplasma genitalium [M. genitalium] in PCa and the controls group with benign prostate hyperplasia [BPH] in Shohada hospital. A total of 124 paraffin-embedded prostate tissues [62 PCa patients and 62 controls with BPH] were included in this study. The subjects'specimens were investigated by the polymerase chain reaction method for the presence of U. urealyticum and M. genitalium DNA. U. urealyticum was detected by standard PCR in 1.61% of the 62 PCa patients and there was no DNA U. urealyticum in the 62 controls with BPH. No M. genitalium was detected by standard PCR in the prostates of 124 paraffin-embedded prostate tissues. According to our results, there is no association between M. genitalium and U.urealyticum with PCa. We recommend further studies using a large sample to determine role of Ureaplasma and Mycoplasma in PCa because understanding the role of infectious agents on PCa might be useful for developing new therapeutic approaches and prevention of PCa
ABSTRACT
The present study was aimed to investigate molecular diversity of Echinococcus gmnulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nod1 in Iran. Forty seven human hydatid cysts were collected through surgery from two hospitals in Tehran during 2010-2012. To determine the fertility of protoscoleces, the cyst fluids were subjected to morphological microscopic examinations. Protoscoleces were removed from each cyst and their total genomic DNAs were extracted. PCR was performed to amplify fragments of 450 and 400 base pair [bp] for cox1 and nod1 genes, respectively. Genotype diversity and sequence variation of the strains were studied by bioinformatics software and in comparison with those mtDNA sequences already dposited in GenBank. Sixteen, [53.3%], 13 [43.3%], and 1 [3.3%] samples were related to lung, liver, and spleen, respectively. The remained 17 unfertile samples were excluded from the study. From the 29 isolates, 86.7% [n=26] and 10% [n=3] were related to G1, and G3 genotypes, respectively. The sole isolate with G6 genotype was obtained from lung sample. Analysis of concatenated sequences of cox1+nad1 indicated the presence of 11 haplotypes among our strains that were related to genotypes G1 [n=9], G3 [n=1] and G6 [n=1]. In consistent to other reports from Iran, genotypes G1, G3, and G6 were observed in our human isolates. The rate of G3 genotype was however higher than other studies implying that human can be considered as a new appropriate host for G3 genotype. Further studies with more sample size from different geographic areas of Iran are needed for E. granulosus mapping
ABSTRACT
Acinetobacter baumannii is a Gram-negative coccobacillus and one of the most opportunistic pathogens responsible for serious infections in hospitalized patients. During a 12 months study, 221 clinical isolates and 22 environmental Acinetobacter baumannii isolates were collected. In vitro susceptibility of Acinetobacter baumannii isolates to 13 antimicrobial agents: amikacin; cefepime; ceftazidime; ciprofloxacin; meropenem; piperacillin/tazobactam; sulfamethoxazole/ trimethoprim; imipenem; tigecycline; colistin; gentamycin; ceftriaxone; levofloxacin was performed by the disk diffusion method. Also Minimum Inhibitory Concentration [MICs] of imipenem; levofloxacin and cefepime was performed by the E-test according to Clinical and Laboratory Standards Institute [CLSI] criteria, blaOXA-23.; blaOXA-24-, blaOXA-58, blaOXA-51genes were detected by polymerase chain reaction and sequencing. The result of antimicrobial susceptibility test of clinical isolates by the disk diffusion method revealed that all strains of Acinetobacter baumannii were resistant to piperacillin/tazobactam. The rates of resistance to the majority of antibiotics tested varied between 69% and 100%, with the exception of tigecycline and colistin. Of 221 isolates tested 99 [44.8%] were XDR. All strains carried a blaOXA-51, like gene. blaOXA-23 gene was the most prevalent among blaOXA-types. Colistin and tigecycline can be effective drugs for treatment of Acinetobacter baumannii infections Continuous Surveillance for Acinetobacter baumannii multidrug-resistant strains is necessary to prevent the further spread of resistant isolates