ABSTRACT
Statement of the Problem: A great challenge in periodontal therapy is the regeneration enhancement of osseous defects through applying osteoinductive materials. Demineralized freeze-dried bone allograft [DFDBA] has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. Calcium hydroxide [Ca[OH]2] is an available well-known material in dentistry, which induces hard tissue formation
Purpose: This study evaluated the efficiency of combination of DFDBA and Ca[OH]2 in improving the quality of osteoinduction of DFDBA
Materials and Method: Human bone marrow-derived mesenchymal stem cells were taken from volunteers' iliac crest. Cell proliferation was determined by MTT test at 18, 24 and 48 hours post-culture in 10 groups. The employed material were 0.5, 1.0 mg/ml Ca[OH]2 in two forms of suspension and pH-adjusted solution, 10mg/ml DFDBA per se and in combination with 0.5 and 1.0 mg/ml Ca[OH]2. Mineralization was assessed by Alizarin red staining in 10 mg/ml DFDBA, DFDBA+ 0.5 and 1 mg/ml Ca[OH]2 in solution and suspension forms. The data were statistically analyzed by using one-way ANOVA and Tukey's post-hoc test [p< 0.05]
Results: The pH-adjusted solutions exhibited better cell proliferation compared with the suspension groups. The combination of 0.5mg/ml Ca[OH]2 solution and DFDBA increased the cell proliferation and mineralization compared with DFDBA per se [p= 0.033]
Conclusion: The combination of Ca[OH]2 with DFDBA improved the osteoinductivity of DFDBA
ABSTRACT
OBJECTIVE: Combined oral contraceptives (COCs) have some adverse effects on the serum lipid profile. Because hyperlipidemia is one of the risk factors in cardiovascular diseases, lipid abnormalities should be evaluated in women consuming COCs. Vitamins E and C are known to have beneficial effects on serum lipid profiles. Therefore, in this study, we evaluated the effects of vitamins E and C on serum lipids in women using COCs. METHODS: The study compared changes in lipid parameters with and without vitamin therapy in women consuming COCs compared to those of a control group (40 non-contraceptive users or NCU) for 4 weeks. Total cholesterol and triglyceride, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) levels along with HDL/LDL ratios were measured for all participants. RESULTS: COC users experienced significantly higher increases in the levels of triglycerides and LDL than non-users (p<0.05). However, no significant differences were noted in the total cholesterol and HDL levels. In the treated COC group receiving vitamins E and C, the HDL level and the HDL/LDL ratio increased and the LDL and triglycerides levels decreased significantly compared with those of the other groups. CONCLUSION: The results of our study indicate that supplementation with antioxidant vitamins E and C restores a normal lipid profile in COC users.