ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Percoll selection technique on normal morphology and acrosin activity of human spermatoza.</p><p><b>METHODS</b>The sperm morphology and sperm acrosin activity were analyzed by automated sperm morphology analyzer(ASMA) and spectrocolorimetry.</p><p><b>RESULTS</b>The normal morphology sperm rate and acrosin activity were significantly increased after Percoll selection technique (P < 0.001).</p><p><b>CONCLUSION</b>Percoll selection technique could affect normal morphology sperm ratio and acrosin activity.</p>
Subject(s)
Adult , Humans , Male , Acrosin , Metabolism , Centrifugation, Density Gradient , Fertilization in Vitro , Spermatozoa , Cell BiologyABSTRACT
AIM: To study the molecular mechanism of reproduction dysfunction in pubertal diabetic rat, the level of 5?-reductase (type 2) mRNA in testis, epididymis and prostate in diabetic rat was detected. METHODS: The gene expression of 5?-reductase (type 2) was detected by Northern blot. RESULTS: In the caput of the epididymis, the expression of 5?-reductase (type 2) mRNA of D and ID groups was less than that of C group. CONCLUSION: The decreased expression of 5?-reductase (type 2) results in the decreased production of dihydrotestosterone, which influences the development and function of reproduction system in pubertal rats. [
ABSTRACT
AIM: To study the dynamic changes of free radicals and antioxidative system of prostate in rats with short-term diabetes. METHODS: 48 Wistar male rats were divided into 6 groups at random (8 rats in each group). One group were injected(ip) with sodium citrate buffer to be the control group, the other 5 groups were injected (ip) with steptozocin(STZ) 60mg/kg body weight which were called diabetes group. Rats were killed after 1, 3, 5, 7 and 14 days; superoxide dismutase(SOD), malondialdehyde(MDA), glutathione(GST), glutathione s-transferase(GST), glutathione peroxidase(GSH-px), nitric oxide (NO) and nitric oxide synthase(NOS) of prostate were examined. RESULTS: The levels of MDA, GSH-px and NO in prostate homogenate of diabetes group were higher evidently as compared with control levels. The activities of SOD, GST, NOS and the levels of GSH in prostate homogenate were increased obviously after injection of STZ with control levels, then returned near to control values. CONCLUSION: The evidences mentioned above indicate that high-level of free radicals and decrease in antioxidative system rendere the prostate cells to be in oxidative stress.