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Objective To compare the levels of S100+ DC,CD83 + DC and IL-23P19 between the patients with mucosa of ulcerative colitis ( UC ) and those with irritable bowel syndrome ( 0BS ).Methods Paraffin-embedded colon tissue from colonoscopic biopsy was detected in sixty UC and sixty IBS cases for the positivity of SI00 + DC,CD83 + DC and IL-23P19 using immunohistochemical SP method.Results There was no significant difference in the positive rates of S100 + DC and CD83 + DC between the two groups ( P ＞ 0.05 ),while significant difference in IL-23P19 level was found between the two groups( 85.0％ vs.70.0％ ; x2 =3.87,P ＜0.05 ).The infiltration densities of S100 + DC,CD83 + DC and expression of IL-23 P19 in the mild and severe UC patients were 24.43 ± 8.26/mm2,5.23 ± 1.66/mm2,95.03 ± 18.39/mm2,and 36.13 ± 11.36/mm2,10.13 ±3.29/mm2,133.38 ± 25.32/mm2,respectively.The difference between the mild and severe UC patients was significant ( P ＜ 0.05 ).No significant difference was found regarding the above three parameters between severity-stratified subgroups of the UC patients ( P ＞ 0.05 ).Conclusion In UC colon tissues,the infiltration density and activity of DC were increased.IL-23P19 level was elevated.The levels of S100 + DC,CD83 + DC and IL-23P19 were increased with the advancement of UC disease.
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Objective:To investigate the clinical characteristic of gastritis verrucosa,and improving the recognition and treatment of this disease thereof.Methods:Six hundred and fifty three patients with gastritis verrucosa,who were diagnosed in Tianjin general hospital from March 2003 to February 2009,were studied.All of these patients were examined by electronic gastroscope and conducted biopsy.Patients were detected helicobacter pylori(HP)using 13C urea breath test.Fifty eight patients with HP-positive and fifty patients with HP-negative(moderate and serious grade),were collected and divided into two groups respectively.The patients in HP-positive groups were given lanseprazole(group A)or rabeprazole(group B).Furazolidone and amoxicilin were given in both groups.The patients in HP-negative groups were administered lanseprazole(group C)or cimetidine(group D).The effects of the treatment were observed in this study.Results:There was no specificity in clinical characteristic of gastritis verrucosa.The pathological changes of gastritis verrucosa were mostly located antrum.The pathological manifestations were chronic inflammation and chronic active inflammation with intestinal metaplasia or atypical hyperplasia.The positive rate of HP infection was 72.74%.There was significant different in the relationship between different levels of inflammation and HP infection(P ＜ 0.05).The effect of treatment was better in group A than that of group B(P ＜ 0.05);and better in group C than that in group D(P ＜ 0.05).Conclusion:There was a correlation between gastritis verrucosa and HP infection.The higher positive rate of HP was correlated with the degree of erosion.For patients with HP-positive,it is better to use the treatment of lansoprazole,amoxicillin and furazolidone.For patients with HP-negative,treatment with lanseprazole was better than cimetidine.
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Objoctive:To investigate the expression of transforming growth factor-β(TGF-β)isoforms in colonic mucosa of ulcerative colitis(UC),and its role in the incidence of UC thereof.Methods:The expression of TGF-β isoforms was detected in colonic mucosa by immunohistochemical Envision method in UC patients,and the patients with irritable bowel syndrome (IBS)were used as controls.The relationship was analyzed between the expression of TGF-β isoforms and the degree of severity and extent of the disease.Results:There were statistical differences in the expression of TGF-β1 and TGF-β2 between the active stage UC,remission stage UC and IBS groups(P ＜ 0.01).The expression was significantly higher in active stage UC group than that in remission stage UC and 1BS groups(P＜ 0.01).The expression was significantly higher in remission stage UC group than that in IBS group(P ＜ 0.01).However,there was no statistical difference in the expression of TGF-β3 between active stage UC,remission stage UC and IBS groups(P＞ 0.05).The expression of TGF-β1 and TGF-β2 had positive correlation with the degree of sevefity(P ＜ 0.05).However,the expression of TGF-β3 had no linear correlation with the degree of severity(P ＞ 0.05).There was no linear correlation on the expressions of TGF-β1,TGF-β2 and TGF-β3 with extent of the disease(P ＞ 0.05).The degree of severity had positive correlation with extent of the disease(P ＜ 0.05).Conclusion:The expressions of TGF-β1 and TGF-β2 were enhanced in colonic mucosa of UC,and were correlated with the pathogenesis of UC.The expressions of TGFβ1 and TGF-β2 may serve as markers for assessing disease activity of UC.
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Objective To observe the effects of different H.pylori strains (cagA + and cagA -) infection on Fas/FasL expression and the pathogenesis of gastirc carcinoma. Methods Gastric biopsy specimens were taken from 80 patients, and these patients were divided into 9 groups according to the H E staining and H.pylori infection. H.pylori (+) groups were subdivided into cagA + and cagA - groups. RUT, PCR and histology were used to detect H.pylori and PCR for cagA detection. Immunohistochemical staining was used to detect Fas/FasL expression. Results The infection rate of H.pylori was 60.0%, and rate of cagA(+) is 90.47%. H.pylori (+) specimens express Fas/FasL more higher than H.poylori (-) ones ( P 0.05). Conclusions Our data suggest that H.pylori infection can induce the expression of Fas/FasL in gastric epithelial cells. Gastric adenocarcinoma express high amount of FasL, which may plays an important role in immune escape of stomach cancer.