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Objective To explore the clinical manifestations and endoscopic characteristics of eosinophilic gastroenteritis(EG) with massive ascites as the initial symptom and accumulate the experiences of EG for early diagnosis and treatment.Methods Clinical characteristics,laboratory examination,endoscopic features and treatment methods of 6 cases of pediatric patients with EG from Nanjing Children's Hospital Affiliated to Nanjing Medical University from May 2009 to Dec.2012 were analyzed.Results The main clinical manifestations of 6 cases with EG serous type were abdominal distension,ascites.Peripheral blood eosinophil (EOS) counts was increased significantly (5.1 × 109/L-20.6 × 109/L).A large number of EOS were found in ascitic fluid of 6 patients.Endoscopic manifestations was gastric mucosal hyperemia and edema in the all of 6 patients,and furthermore,gastric mucosa erosion in 2 cases,esophagus mucosa rough edema in 1 case,duodenal mucosal hyperemia and edema in 3 cases,duodenal nodular hyperplasia in 1 case.The mucosa pathological examination of 6 cases showed eosinophilia.Symptoms in patients were relieved after avoiding suspicious food and oral corticosteroids treatment after 1 week.Conclusions EG serous type patients in children are massive ascites as the initial symptom.Ascitic fluid and peripheral blood EOS counts are increased significantly and endoscopic feature is nonspecific,mucosal hyperemia,edema,erosions,nodular hyperplasia.EOS infiltration is observed in the gastrointestinal mucosa propria by multipoint biopsy.Therapeutic effect of diet therapy and the treatment of glucocorticoid is good.
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Objective To investigate the influence of Helicobacter pylori(Hp) infection on gastric mucin MUC5AC and MUC6 expression in children.Methods Sixty-six cases with gastric biopsy specimens were obtained from 66 children undergone gastroscopy from Jan.2005 to Jun.2006 for episodic or continuous abdominal pain,nausea,vomiting,abdominal distension,retching and dyspepsia,and so on.Among these children,39 cases were male and 27 cases were female,owning a average age(8.8?3.0)years.These specimens were divided into 2 groups followed by the presence of Hp,which was detected by rapid urease tests and Hp-PCR.Gastric mucin MUC5AC and MUC6 mRNA were also measured by reverse transcription PCR(RT-PCR),and Hematoxylin and Eosin staining were used for pathology observation.Comparisons between every groups were performed using t test and ?2 test,and statistical significance was defined as P
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Objective To determine the changes of interleukin-12(IL-12) and IL-12 mRNA in gastric mucosa of children with helicobacer pylori (Hp) infection,and to study the effects of Hp infection on the expression levels of IL-12 and IL-12 mRNA,and to evaluate its possible roles in the pathogenesis of gastric mucosal inflammation in Hp related gastroduodenal diseases.Methods Biopsy specimens were taken from the antral mucosa on endoscopy in patients with or without Hp infection, which were diagnosed by urease test and Giemsa staining. The expression levels of IL-12 and IL-12 mRNA in gastric mucosa were determined by ELISA and RT-PCR.Results Inflammation of gastric antral mucosa was more severe in Hp-positive mucosa .The expression levels of IL-12 and IL-12 mRNA in Hp-positive mucosa were (66.42?15.15) ng/g and (59.21?15.03)%,which were more than those in (Hp-negative )(22.22?8.79) ng/g and (17.94?7.39)%(P