Microbiology of burn patients of civil hospital Karachi

After initial period of shock, infection remains a major and most serious complication in burn patients. The flora of the burn wound is constantly changing and the potential risk of burn wound sepsis persists until complete healing of burn wound. In burn patients most of the infections are thought to be of nosocomial origin. Gram positive cocci are considered with increasing frequency in nosocomial infection. Gram negative organism cause trouble in different ways, pus containing toxins convert partial thickness burns into full thickness burn, causing general illness, failure of graft taking and most important is septicemia Pseudomonas aeruginosa is highly pathogenic in the injured immuno suppressive burn patients. Most common isolates belongs to family Enterobacteriacae i.e. 36 [63.3%]. Second common isolates were belonging to Genus Staphylococci 15 [26%]. The prevalence of Pseudomonas aeruginosa was relatively less that was 07 [10.4]. Gram positive Staphylococci were 100% sensitive to Vancomycin, Chloramphenicol, and Imipenem. Pseudomonas aeruginosa were highly sensitive to Ciprofloxacin and Amikacin i.e. 83.3% Imipenem was highly effective against [97.6%] isolates of Enterobacteriacae. Ciprofloxacin was the second most effective drug that was effective against 87.5%isolates of Enterobacteriaicae

Role of enterobactereacae in burn wound sepsis in Karachi

The over all prevalence of the Enterobacteriacae in present study was more than 50% in which the most prevalent organisms belongs to Proteus Spp. [38,3%] that followed by Enterobacter Spp. [22.4%]. The prevalence of E. coli and Kleb, Pneumoniae was 10%, Imipenem was the most effective [98%] drug against Enterobacieieacae. Ciprofloxacin was the second most effective [82%] drug. Amikacin and fourth generation Cefepirne were the third effective [>70%] drugs against these bacteria. Ciprofloxacin was the less effective [60-64%] drug against KIeb. pneumonia and E. coli than the rest of the isolates of Eiiterobactereacae. Arnikacin [49%] and Cefepime [44%] were less effective against Proteus Spp. More than 50% isolates were sensitive to Piperacillin, Gentamicin and Tobramycin. Amoxicillin and Augmentin were ineffective against these bacteria in this study

Multi drug resistant pseudomonas aeruginosa: a nosocomial infection threat in burn patients

Pseudomonas aeruginosa remains the leading pathogen causing burn wound infection. It is found as major colonizer of the burn wound because it thrives on moist burn wound surface and survives well in the hospital environment, once it is established, it can persist for months within a unit, and poses as multi drug resistant nosocomial infection threat for patients being treated there. The emergence of multi drug resistant Pseudomonas aeruginosa in burn wound is becoming a challenging problem in infection control programmes. A total of 44 isolates of Pseudomonas aeruginosa were recovered from burn patients. Most of them were resistant to multiple antibiotics. Their sensitivity against Imipenem was over all better than the other drugs i.e. 77.3%. Ciprofloxacin was the second most effective drug against this organism with a sensitivity of 54.5% while a 4th generation cephalosporin, Cefepime was effective against 22 [50%] isolates. About 30% Pseudomonas aeruginosa were sensitive to Amikacin. Aztreonam showed inhibitory activity against [6.8%] strains. Piperacillin activity was 18.2%. The efficacy of Cefutaxime was 4.5%. Chloramphenicol and Septran were 100% inactive against Pseudomonas infection while > 95% strains of Pseudomonas aeruginosa were resistant to Tobramycin